Nuray ÇİFTÇİ, Fahri KARAYAKAR, Bedii CİCİK

Effects of Clinoptilolite on Copper Accumulation of Oreochromis niloticus

The copper accumulation in liver and gill tissues of Oreochromis niloticus, exposed to 2 ppm Cu and 1g/L clinoptilolite singly and to the same concentrations of their mixture over 24, 48, 72 and 96 hours was studied. ICP-AES spectrophotometer techniques were applied in determining tissue copper levels. Statistical evaluation of the experimental data was carried out by Student Newman Keul’s procedure. No mortality was observed during the experiments. Copper accumulation was lower in metal-clinoptilolite mixture group than metal singly group in gill tissue while no accumulation in both experimental groups in liver tissue (P<0.05). In addition, the copper level in the liver was lower in all experimental groups than in the control (P<0.05). Low Cu accumulation in gill tissue exposed in mixture groups can be explained by copper adsorption with chelating agent. The decrease of Cu reserves in the liver can be expressed by increase of copper-containing enzyme and protein synthesis.

Keywords:

O. niloticus, Copper Clinoptilolite, Tissue, Accumulation,

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Batchelder, T.L., Alexander, H.C. & McCarty, W.M. (1980). Acute Fish Toxicity of the Versene Family of Chelating Agents, Bull. Environ. Contam. Toxicol., 24, 543-549.
Çoğun, H.Y. & Şahin, M. (2012). The Effect of Zeolite on Reduction of Lead Toxicity in Nil Tilapia (Oreochromis niloticus Linnaeus, 1758), Kafkas Unıversitesi Veteriner Fakültesi Dergisi, 18, 135-140.
Fromm, P.O. & Schiffman, R.H. (1958). Toxic Action of Hexavalent Chromium on Largemouth Bass. Journal of Wildlife Management, 22(1), 40-44.
Gopal, R., Narmada, S., Viayakumar, R. & Jaleel, C.A. (2009). Chelating Efficacy of CaNa2 EDTA on Nickel-Induced Toxicity in Cirrhinus mrigala (Ham.) trough its Effects on Glutathione Peroxidase, Reduced Glutathione and Lipid Peroxidation, C. R. Biologies, 332, 685-696.
Grosell, M. (2012). Copper, In: Homeostasis and Toxicology of Essential Metals. (Eds.Chris, M. Wood, Anthony, P. Farrell and Colin J. Brauner), Fish Physiology, 31A, Academic Press, USA, 494.
Hansten, C., Heino, M. & Pynnönen, K. (1996). Viaility of Glochidia of Anodonta anatina (Unionidae) Exposed to Selected Metals and Chelating Agents, Aquatic Toxicology, 34, 1-12.
Heath A.G. (1995). Water Pollution and Fish Physiology, 2nd edition, CRC Press, New York.
Hung,Y. (1982). Effects of Temperature and Chelating Agents on Cadmium Uptake in the American Oyster, Bulletin of Environmental Contamination and Toxicology, 28, 546-551.
Jain, S.K. (1999). Protective Roles of Zeolite on Short and Long Term Lead Toxicity in Teleost Fish Heteropneustes fossilis, Chemosphere, 39(2), 247-251.
James, R. (2000). Effect of Zeolite on Reduction of Cadmium Level in Water and Improvement of Haematological Parameters in Oreochromis mossambicus (Peters), Indian Journal of Fisheries, 47(1), 29-35.
James, R. & Sampath K. (1999). Effect of Zeolite on the Reduction of Cadmium Toxicity in Water and a Freshwater Fish, Oreochromis mossambicus, Bulletin of Environmental Contamination and Toxicology, 62, 222-229.
James, R. & Sampath, K. (1999). Effect of Zeolite on Reduction of Cadmium Toxicity: An Experimental Study on Element Uptake and Growth in Heteropneustes fossilis (Bloch), J. Aqua. Trop., 14, 65-74.
James, R., Sampath, K., Jeyamari, R. & Selvamani, P. (2004). Effect of Zeolite (Sodium aluminosilicate) on the Removal of Copper From Water and Fish and an Improvement of RNA:DNA Ratio in Oreochromis mossamicus (Peters), Ecohydroiology &Hydroiology, 4, 57-65.
James, R., Sampath, K. & Selvamani, P. (1998). Effects of EDTA on Reduction of Copper Toxicity in Oreochromis mossamicus, Bulletin of Environmental Contamination and Toxicology, 60 487-493.
Kumar, B., Mukherjee, D.P., Kumar, S., Mishra, M., Prakash, D., Singh, S.K. & Sharma, C.S. (2011). Bioaccumulation of heavy metals in muscle tissue of fishes from selected aquaculture ponds in east Kolkata wetlands, Annals of Biological Research, 2(5), 125-134.
Langston, W.J., Chesman, B.S., Burt, G.R., Pope, N.D. & McEvoy, J. (2002). Metallothionein in Liver of Eels Anguilla anguilla from the Thames Estuary: An Indicator of Environmental Quality, Marine Environmental Research, 53(3), 263-293.
Marani, D., Macci G. & Pagano M. (1995). Lead Precipitation in The Presence of Sulphate and Carbonate, Testing of Thermodynamic Predictions, Water Research, 29(4), 1085-1092.
Maruldalı, O. (2010). Kurşun ve Kurşun-Selenyum’un Etkisine Bırakılan Oreochromis niloticus (L.)’da Kurşunun Karaciğer, Böbrek, Beyin ve Solungaç Dokularında Birikimi ve AChE Aktivitesine Etkisi, Çukurova Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı Yüksek Lisans Tezi, Adana, 66 s.
Palaniappan, PL. RM., Krishnakumar, N. & Vadivelu, M. (2009). Bioaccumulation of Lead and the Influence of Chelating Agents in Catla catla Fingerlings, Environmental Chemistry Letters, 7, 51-54.
Papini M. P., Kahie Y. D., Troia B. & Majone M. (1999). Adsorption of Lead at Variable pH Onto a Natural Porous Medium: Modeling of Batch and Column Experiments, Environmental Science and Technology, 33, 3357-4464.
Patterson, J.W. (1985). Industrial Wastewater Treatment Tecnology, 2nd ed. Stoneham, M. A: Butterworths Pulishers.
Patterson, J. W., Minear, R. A., Gasca, E. & Petropoulou, C. (1998). Industrial Discharges of Metals to Water, In: Metals in Surface Waters (H.E. Allen, A.W. Garrison and G.W. Luther, eds), Ann Arbor Press, Chelsea, MI, 37-66.
Reyhan, İ.Ç. (2014). Oreochromis niloticus’da Bakır ve Kadmiyum Birikiminde Kalsiyum ve Zeolitin Etkileri, Kilis 7 Aralık Üniversitesi Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Yüksek Lisans Tezi, Kilis, 53 s.
Shukla,V., Dhankhar, M., Prakash, J. & Sastry, K.V. (2007). Bioaccumulation of Zn, Cu and Cd in Channa punctatus, Journal of Environmental Biology, 28(2), 395-397.
Sivakumar, S., Khatiwada, C.P. & Sivasubramanian, J. (2012). Bioaccumulations of Aluminum and the Effects of Chelating Agents on Different Organs of Cirrhinus mrigala, Environmental Toxicology and Pharmacology, 34, 791-800.
Smith, S. R. (1996). Agricultural Recycling of Sewage Sludge and the Environment, CAB International,Wallingford, UK, 119-151.
Wicklund, A. & Runn, P. (1988). Calcium Effects on Cadmium Uptake, Redistribution, and Elemination in Minnows, Phoxinus phoxinus, Acclimated to Different Calcium Concentrations. Aquatic Toxicology, 13, 109-122.