Deneysel Sepsis Modelinde Antibiyotik Tedavisine Eklenen Farklı Dozlardaki Ozonun Sitokin Düzeylerine Etkisi

Bu çalışmada E.coli endotoksini ile oluşturulan deneysel sepsis modelinde antibiyotik tedavisine eklenen ozon tedavisinin proinflamatuvar ve antiinflamatuvar sitokin düzeylerinde yaptığı değişiklikleri incelemeyi amaçladık. Her birinde 10 rat olan 6 deney grubunun 5 tanesinde intraperitoneal E.coli endotoksini injeksiyonuyla sepsis oluşturuldu. İlk 3 grupta antibiyotik tedavisine 0.6 mg/kg, 0.9 mg/kg ve 1.1 mg/kg dozlarında ozon tedavisi eklenirken, 4. gruba sadece antibiyotik tedavisi verildi. 5. gruba tedavi verilmedi. 6. gruba ise diğer gruplarla eş zamanlı olarak intraperitoneal serum fizyolojik enjeksiyonu yapıldı. Tüm tedavilere 5 gün boyunca devam edildi. 6. günde sakrifiye edilen ratlardan alınan kanların serumlarında IL-1, IL-10 ve TNF-alfa düzeylerini çalışıldı. Ozon tedavisi verilen tüm gruplardaki proinflamatuvar sitokin düzeylerinin (IL-1, TNF-alfa) diğer gruplara göre anlamlı olarak düşük olduğunu tespit edildi. Ozon tedavisi verilen grupların kendi aralarında yapılan karşılaştırmada, IL-1 düzeylerinde anlamlı bir fark olmadığı, TNF-alfa düzeylerinin ise daha düşük doz verilen iki grupta (0.6 mg/kg ve 0.9 mg/kg) yüksek doz verilen gruba (1.1 mg/kg) göre anlamlı olarak düşük olduğu gözlemlendi. Antiinflamatuvar bir sitokin olan IL-10’un serum düzeylerinde ise gruplar arasında anlamlı bir farklılık tespit edilmedi. Sonuç olarak, sepsiste antibiyotik tedavisine eklenen ozon tedavisinin inflamatuar süreci baskılayarak sağ kalım oranlarına olumlu etki yapabileceği sonucuna varıldı.

Anahtar Kelimeler:

IL-1, IL-10, Sepsis, Ozon, TNF-alfa

Effects of Different Ozone Doses Added to the Antibiotic Treatment on Cytokine Levels in Experimental Sepsis Model

We aimed to examine the changes caused by ozone therapy added to the antibiotic treatment on proinflammatory and anti-inflammatory cytokine levels in an experimental sepsis model formed with E. coli endotoxin. Rats were divided into 6 groups of 10 rats. Sepsis was formed by dosing 5 groups of rats with intraperitoneal E. coli endotoxin injection. For the first 3 groups, 0.6 mg/kg, 0.9 mg/kg, and 1.1 mg/kg doses of ozone therapy were added to the antibiotic treatment and group-4 only received antibiotic treatment. Group-5 was not treated. Group-6 received intraperitoneal serum physiologic injection simultaneously with the other groups. All treatments were sustained for 5 days. IL-1, IL-10, and TNF-alpha levels were detected in blood serum taken from rats sacrificed on day 6. It was seen that IL-1, TNF-alpha levels are significantly lower than the levels in other groups that received ozone therapy. In the comparisons amongst the groups receiving ozone therapy, it was observed that IL-1 levels do not have a significant difference and TNF-alpha levels are significantly lower in the two groups receiving lower doses than the group receiving a higher dose. There were no significant differences detected between groups at serum levels of IL-10 which is an anti-inflammatory cytokine. It was concluded that ozone added to the antibiotic treatment in sepsis could have a positive effect on survival rates by suppressing inflammatory process.

Keywords:

IL-1, IL-10, Sepsis, Ozone, TNF-alpha,

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