Hamam HAMAM, Halit DEMİR, Mesut AYDIN, Canan DEMİR

Sirotik Karaciğer Hastalarında Bazı Antioksidan Aktivitelerin (Süperoksit Dismutaz, Katalaz, İndirgenmiş Glutatyon) ve Oksidatif Stres Düzeyinin (Malondialdehit Asit) Belirlenmesi

Amaç: Bu çalışmanın amacı karaciğer sirozlu hastaların kan serumunda lipid peroksidasyon (MDA) ve indirgenmiş glutatyon (GSH), katalaz (CAT) ve süperoksit dismutaz (SOD) gibi antioksidanların düzeylerini belirlemektir. Yöntemler: Bu çalışmada serum malondialdehit asit (MDA) düzeyleri, süperoksit dismutaz (SOD), indirgenmiş glutatyon (GSH) ve katalaz (CAT) aktiviteleri spektrofotometrik olarak ölçüldü. Bulgular: Hasta gruplarında SOD, CAT ve GSH aktiviteleri sağlıklı kontrol grubuna göre anlamlı olarak azaldı (p<0.05). MDA düzeyleri hasta grubunda sağlıklı kontrol grubuna göre anlamlı olarak yüksekti (p<0.05). Sonuç: Sonuç olarak, bu çalışmada oksidatif stres karaciğer sirozu gelişiminde önemli rol oynayabilir. Bu çalışma karaciğer sirozunda MDA, SOD, CAT ve GSH ilişkilerini gösteren ilk çalışmadır. Karaciğer sirozunda antioksidan enzimleri ve oksidatif stres durumunu araştırmak için daha ileri çalışmalar gereklidir.

Anahtar Kelimeler:

Karaciğer Sirozu, SOD, GSH, CAT, MDA

Determination of some Antioxidant Activities (Superoxide Dismutase, Catalase, Reduced Glutathione) and Oxidative Stress Level (Malondialdehyde Acid) in Cirrhotic Liver Patients

Objective: The aim of this study was to determine the levels of malondialdehyde (MDA) and antioxidants such as reduced glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) in the blood serum of liver cirrhosis patients.Methods: İn This investigation, we took blood from 31 healthy individuals, and 30 patients with Cirrhosis in both males and females. In this study, serum MDA levels, SOD, GSH, and CAT activities were measured spectrophotometrically. In paired group comparisons in terms of continuous variables; the T-test was utilized where normal deviation was achieved, and Mann-Whitney U statistics was utilized where it was not. In addition, ROC curve analysis was performed to evaluate their performance in differentiating the patient group from the control group.Results: SOD, CAT, and GSH activities were significantly decreased in the patient groups compared to the healthy control group (p<0.05). MDA levels were significantly higher in the patient group compared to the healthy control group (p <0.05).Conclusion: In conclusion, in this study, oxidative stress may play an important role in the development of liver cirrhosis. This study is the first one to show the relationships of MDA, SOD, CAT, and GSH in liver cirrhosis. Further studies are essential to investigate antioxidant enzymes and oxidative stress status in liver cirrhosis.

Keywords:

Liver Cirrhosis, SOD, GSH, CAT, MDA,

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