Ali TAGHİZADEHGHALEHJOUGHİ, Muhammet Emin NALDAN

The study of diazepam, pregabalin and glucose effect on glutamate toxicity: In vitro study

In this study we used pregabalin, diazepam and glucose for evaluating which one are more effective agents against glutamate induced neurotoxicity. After cortex culture preparation glutamate toxicity induced by adding 10-5 mM glutamate to each wells except negative control group. After 10 min different dose of diazepam, pregabalin and glucose were added for 24 h and then 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Total Antioxidant Status (TAC) and Total oxidant status (TOS) assays were done. Diazepam, dose dependently shows neuroprotective effects and low dose of diazepam did not increase cell viability higher than 80 %. Combination groups only in high dose increased neurons viability. Total Antioxidant capacity of Pregabalin also show correlation with diazepam groups and highest dose of both show nearest value to negative control group. In addition, lowest and highest oxidant level were gained by the negative and positive control groups respectively. Except high dose of combination group, all groups show statically difference in compare with the negative control group P

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Plitman E, Nakajima S, de la Fuente-Sandoval C, et al. Glutamate-mediated excitotoxicity in schizophrenia: A review. Eur Neuropsychopharmacol. 2014;24:1591-605.

Blacker CJ, Lewis CP, Frye MA, et al. Roles of metabotropic glutamate receptors (mGluRs) in bipolar disorder: a systematic review. Bipolar Disorders 2015; 17:124-5.

Ramadan S, Lin A, Stanwell P. Glutamate and glutamine: a review of in vivo MRS in the human brain. NMR in Biomedicine. 2013;26:1630-46.

Rousseaux CG. A Review of Glutamate Receptors II: Pathophysiology and Pathology. Journal of Toxicologic Pathology. 2008;21:133-73.

Walker JE. Glutamate, Gaba, and Cns Disease - a Review. Neurochemical Research. 1983;8:521-50.

Zavodnick AD, Ali R. N-acetylcysteine and metabotropic glutamate receptors: implications for the treatment of schizophrenia: a literature review. Psychiatric Quarterly. 2014;85:177-85.

Balcar VJ, Takamoto A, Yoneda Y. Neurochemistry of L-glutamate transport in the CNS: A review of thirty years of progress. Collect. Czech. Chem. Commun. 2001;66:1315-40.

Grados MA, Atkins EB, Kovacikova GI, et al. A selective review of glutamate pharmacological therapy in obsessive-compulsive and related disorders. Psychol Res Behav Manag. 2015;8:115-31.

Meldrum BS. Glutamate as a neurotransmitter in the brain: Review of physiology and pathology. J Nutr. 2000;130:1007s-15s.

Mohler H. GABAA receptors in central nervous system disease: Anxiety, epilepsy, and insomnia. J Recept Signal Transduct Res. 2006;26:731-40.

Brondino N, Fusar-Poli L, Panisi C, et al. Pharmacological modulation of gaba function in autism spectrum disorders: a systematic review of human studies. J Autism Dev Disord. 2016;46:825-39.

Fabritius ML, Strom C, Koyuncu S, et al. Benefit and harm of pregabalin in acute pain treatment: a systematic review with meta-analyses and trial sequential analyses. Br J Anaesth. 2017;119:775-91.

Generoso MB, Trevizol AP, Kasper S, et al. Pregabalin for generalized anxiety disorder: an updated systematic review and meta-analysis. Int Clin Psychopharmacol. 2017;32:49-55.

Ryu JH, Lee PB, Kim JH, et al. Effects of pregabalin on the activity of glutamate transporter type 3. Br J Anaesth. 2012;109:234-9.

Kumar N, Laferriere A, Yu JSC, et al. Evidence that pregabalin reduces neuropathic pain by inhibiting the spinal release of glutamate. J Neurochem. 2010;113:552-61.

Shimizu N, Tsuda M, Suzuki T, Misawa M. Role of metabotropic glutamate receptors in lowered seizure threshold of pentylenetetrazole during diazepam withdrawal. Naunyn-Schmiedebergs Archives of Pharmacology 1998; 358:R184-R184.

Barnes T, Di Sebastiano KM, Vlavcheski F, et al. Glutamate increases glucose uptake in L6 myotubes in a concentration- and time-dependent manner that is mediated by AMPK. Appl Physiol Nutr Metab. 2018;30.

Greenamyre JT, Porter RH. Anatomy and physiology of glutamate in the CNS. Neurology. 1994;44:7-13.

Meldrum BS. Glutamate as a neurotransmitter in the brain: review of physiology and pathology. J Nutr. 2000;130:1007-15.

Kamalak H, Kamalak A, Taghizadehghalehjoughi A. Cytotoxic effects of new-generation bulk-fill composites on human dental pulp stem cells. Cell Mol Biol (Noisy-le-grand). 2018; 64:62-71.

Cunningham MO, Woodhall GL, Thompson SE, et al. Dual effects of gabapentin and pregabalin on glutamate release at rat entorhinal synapses in vitro. Eur J Neurosci. 2004;20:1566-76.

Schuler M, Gudi R, Cheung J, et al. Evaluation of phenolphthalein, diazepam and quinacrine dihydrochloride in the in vitro mammalian cell micronucleus test in Chinese hamster ovary (CHO) and TK6 cells. Mutat Res. 2010;702:219-29.

Ueda Y, Inui A, Mifune Y, et al. The effects of high glucose condition on rat tenocytes in vitro and rat Achilles tendon in vivo. Bone Joint Res. 2018;7:362-72.

Gundogdu G, Miloglu FD, Nalci KA, et al. Protective Effect of Zn2+Loaded Fe3O4-SiO2-NH2-(Zinpyr-1) Nanocomposite on Glutamate Exercise Toxicity in Primary Cortical Neuron Cell Culture. Acta Physiolog. 2017;221:78-9.

Ahiskalioglu A, Ince I, Aksoy M, et al. Comparative Investigation of Protective Effects of Metyrosine and Metoprolol Against Ketamine Cardiotoxicity in Rats. Cardiovasc Toxicol. 2015;15:336-44.

Babateen O, Jin Z, Bhandage A, et al. Etomidate, propofol and diazepam potentiate GABA-evoked GABAA currents in a cell line derived from human glioblastoma. Eur J Pharmacol. 2015;748:101-7.

Brawek B, Loffler M, Weyerbrock A, et al. Effects of gabapentin and pregabalin on K+-evoked 3H-GABA and 3H-glutamate release from human neocortical synaptosomes. Naunyn Schmiedebergs Arch Pharmacol. 2009;379:361-9.

Errante LD, Petroff OA. Acute effects of gabapentin and pregabalin on rat forebrain cellular GABA, glutamate, and glutamine concentrations. Seizure. 2003;12:300-6.

Galeffi F, Schwartz-Bloom RD. Diazepam promotes ATP recovery following oxygen-glucose deprivation in the hippocampal slice. Faseb J. 2000;14:A1500-A1500.

Brekke E, Berger HR, Wideroe M, et al. Glucose and Intermediary Metabolism and Astrocyte-Neuron Interactions Following Neonatal Hypoxia-Ischemia in Rat. Neurochem Res. 2017;42:115-32.

Rotter Sopasakis V, Wickelgren R, Sukonina V, et al. Elevated glucose levels preserve glucose Uptake, hyaluronan production, and low glutamate release following interleukin-1beta Stimulation of differentiated chondrocytes. Cartilage. 2018:1947603518770256.

Eintrei C, Sakoloff E, Smith CB. Effects of diazepam and ketamine administered individually or in combination on regional rates of glucose utilization in rat brain. Br J Anaesth. 1999;82:596-602.