EMİNE KAÇAR, ZÜBEYDE ERCAN, Fatih TAN, Gökhan ZORLU, ÖZGÜR BULMUŞ, İHSAN SERHATLIOĞLU

The effects of nitrofurantoin on rat urinary bladder contraction

Nitrofurantoin is a drug used in the antibacterial treatment of urinary tract infections (UTIs) for over 60 years. The aim of our study was to investigate the possible effects of nitrofurantoin, which is commonly used as a urinary tract antiseptic, on bladder contractions in male rats. Bladder tissues obtained from male Sprague-Dawley rats were used in the study (n=24). After decapitation, bladder tissues were suspended in an isolated organ bath of 5 ml containing a Krebs-Ringer bicarbonate solution by applying a tension of 1.5 grams. Nitrofurantoin was administered to three groups at doses of 50, 500 and 1000 μM, respectively. The area, peak-to-peak (p-p) and frequency values of bladder contractions were analyzed before and after administration of nitrofurantoin. The data obtained from the analysis were evaluated using the Paired T-Test in the IBM SPSS Statistics Software. Nitrofurantoin was observed to have an inhibitory effect on bladder contractions at all doses. The decrease in the area and peak-to-peak values was statistically significant at all doses (P

PDF

___

Masajtis-Zagajewska A, Nowicki M. New markers of urinary tract infection. Clin Chim Acta. 2017;471:286-91.

Cueto M, Aliaga L, Alós JI, et al. Executive summary of the diagnosis and treatment of urinary tract infection: Guidelines of the spanish society of clinical microbiology and infectious diseases (SEIMC). Enferm Infecc Microbiol Clin. 2017;35:314-20.

Geerlings SE. Clinical presentations and epidemiology of urinary tract infections. Microbiol Spectr. 2016;4:5.

Sabih A, Leslie S. Urinary tract infections, complicated. Stat pearls [Internet]. Treasure Island (FL): StatPearls Publishing, 2017.

Claussen K, Stocks E, Bhat D, et al. How common are pulmonary and hepatic adverse effects in older adults prescribed nitrofurantoin? J Am Geriatr Soc. 2017;65:1316-20.

Muller AE, Verhaegh EM, Harbarth S, et al. Nitrofurantoin’s efficacy and safety as prophylaxis for urinary tract infections: a systematic review of the literature and meta-analysis of controlled trials. Clin Microbiol Infect. 2017;23:355-62.

Shakti L, Veeraraghavan B. Advantage and limitations of nitrofurantoin in multi-drug resistant Indian scenario. Indian J Med Microbiol. 2015;33:47781.

Busby-Whitehead JM, Johnson TM. Urinary incontinence. Clin Geriatr Med 1998;14:285-96.

Robinson D, Cardozo L. Estrogens and the lower urinary tract. Neurourol Urodyn. 2011; 30: 754-7.

Edwards JL. Diagnosis and management of benign prostatic hyperplasia. Am Fam Physician. 2008;77:1403-10.

Buzelin JM. Female urinary incontinence. Which assessments? Which treatment?. Ann Urol. 1998;32:83-8.

Van Gool J, Tanagho EA. External sphincter activity and recurrent urinary tract infection in girls. Urology. 1977;10:348-353.

Ando M, Nagamatsu H, Tanizawa A, et al. Clinical analysis of urinary incontinence in the institutionalized elderly. Nihon Hinyokika Gakkai Zasshi. 1991;82:1299-304.

Majeed A, Alarfaj S, Darouiche R, Mohajer M. An update on emerging therapies for urinary tract infections. Expert Opin Emerg Drugs. 2017;22:53-62.

Ahmed H, Davies F, Francis N, et al. Long-term antibiotics for prevention of recurrent urinary tract infection in older adults: systematic review and metaanalysis of randomised trials. BMJ Open. 2017;7:1-7.

Cunha BA, Cunha CB, Lam B, et al. Nitrofurantoin safety and effectiveness in treating acute uncomplicated cystitis (AUC) in hospitalized adults with renal insufficiency: antibiotic stewardship implications. Eur J Clin Microbiol Infect Dis. 2017;36:1213-6.

Price JR, Guran LA, Gregory WT, McDonagh MS. Nitrofurantoin vs other prophylactic agents in reducing recurrent urinary tract infections in adult women: a systematic review and meta-analysis. Am J Obstet Gynecol. 2016;215:548-60.

Weng TI, Chen WJ, Liu SH. Bladder instillation of Escherichia coli lipopolysaccharide alters the muscle contractions in rat urinary bladder via a protein kinase C-related pathway. Toxicol Appl Pharmacol. 2005;208:163-9.

Burnstock G. Introductory overview of purinergic signaling. Front. Biosci. 2011;3:896-900.

Suzuki H, Kokubun S. Subtypes of purinoceptors in rat and dog urinary bladder smooth muscles. Br. J. Pharmacol. 1994;112:117-22

McMurray G, Dass N, Brading AF. Purinoceptor subtypes mediating contraction and relaxation of marmoset urinary bladder smooth muscle. Br. J. Pharmacol. 1998;123:1579-86.

Aronsson P, Andersson M, Ericsson T, Giglio D. Assessment and characterization of purinergic contractions and relaxations in the rat urinary bladder. Basic Clin. Pharmacol. Toxicol. 2010;107:603-13.

Hill WG. Control of urinary drainage and voiding. Clin J Am Soc Nephrol. 2015;10:480-92.

Balachandran AA, Wildman SS, Strutt M, Duckett J. 2016. Is chronic urinary infection a cause of overactive bladder? Eur J Obstet Gynecol Reprod Biol. 201:108-12.

Khasriya R, Sathiananthamoorthy S, Ismail S, et al. Spectrum of bacterial colonization associated with urothelial cells from patients with chronic lower urinary tract symptoms. J Clin Microbiol. 2013;51:2054-62.

Sorrentino F, Cartwright R, Digesu GA, et al. Associations between individual lower urinary tract symptoms and bacteriuria in random urine samples in women. Neurourol Urodyn. 2015;34:429-33.

Vijaya G, Cartwright R, Derpapas A, et al. Changes in nerve growth factor level and symptom severity following antibiotic treatment for refractory overactive bladder. Int Urogynecol J. 2013;24: 523-8.