Cemil Colak, Yeliz Kasko Arici, Sahin Direkel, Ulku Karaman, Emine Yurdakul Erturk

Prevalence of Cyclospora cayetanensis and Cryptosporidium spp. children according to some variables

In this study, the prevalence of Cyclospora cayetanensis and Cryptosporidium spp. were researched in children with parasitological investigations requested for a variety of reasons, regardless of immune status. The stool samples of 1057 pediatric patients with parasitological evaluation requested for a variety of reasons within two years were investigated under a microscopy using native-lugol, sedimentation and modified Kinyoun’s acid-fast stain methods. The mean age of the 1057 pediatric patients participating in the research were 8.07±4.32 years, 11.4% were identified to have coccidian parasites (Cyclospora cayetanensis and Cryptosporidium spp.). Evaluation according to age group found the majority of children positive for Cryptosporidium spp. were in the 6-12 year interval. These parasites were observed more frequently in the spring and summer seasons compared to other seasons. There was a significant correlation between parasite positivity and spring and summer seasons (p

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Bhattachan B, Sherchand JB, Tandukar S, et al. Detection of Cryptosporidium parvum and Cyclospora cayetanensis infections among people living in a slum area in Kathmandu valley, Nepal. BMC Res Notes. 2017;10:464.
Kumar P, Vats O, Kumar D, et al. Coccidian intestinal parasites among immunocompetent children presenting with diarrhea: Are we missing them?. Trop Parasitol. 2017;7:37-40.
Ramirez NE, Ward LA, Sreevatsan S. A review of the biology and epidemiology of cryptosporidiosis in humans and animals. Microbes Infect. 2004;6:773-85.
Clark DP. New insights into human cryptosporidiosis. Clin Microbiol Rev. 1999;12:554-63.
Bern C, Ortega Y, Checkley W, et al. Epidemiologic differences between cyclosporiasis and cryptosporidiosis in Peruvian children. Emerg Infect Dis. 2002;8:581-5.
Herwaldt BL. Cyclospora cayetanensis: a review, focusing on the outbreaks of cyclosporiasis in the 1990s. Clin Infect Dis. 2000;31:1040-57.
Rabold JG, Hoge CW, Shlim DR, et al. Cyclospora outbreak associated with chlorinated drinking water. Lancet. 1994;344:1360-1.
National Microbiology Standards (2015). Microscopic examination of feces. Available from:http://microbiology.thsk.health.gov.tr/File/diagnosisguide/parasitology/UMS-P-OY-01-Parasitological examination of feces pdf
National Microbiology Standards (2015). Trichrome staining. Available from:
National Microbiology Standards (2015). Concentration Methods. Available from: http://microbiology.thsk.health.gov.tr/File/diagnosisguide/parasitology/UMS-P-TP-03- Concentration methods pdf
Caner A, Zorbozan O, Tunalı V, et al. intestinal protozoan parasitic infections in immunocompromised child patients with diarrhea. Jpn J Infect Dis. 2020;73:187-92.
Nahrevanian H, Assmar M, Samin MG. Cryptosporidiosis among immunocompetent patients with gastroenteritis in Iran: a comparison with other enteropathogenic parasites. J Microbiol Immunol Infect. 2007;40:154- 6.
Yilmaz H, Tas-Cengiz Z, Cicek M. Investigation of cryptosporidiosis by enzyme-linked immunosorbent assay and microscopy in children with diarrhea. Saudi medical journal. 2008;29:526-9.
Arslan MO, Sari B, Kulu B, et al. The prevalence of intestinal parasites in children brought to the Kars Maternal and Children's Hospital with complaints of gastrointestinal symptoms. Turkiye Parazitol Derg. 2008;32:253-6.
Aksoy U, Akisu C, Sahin S, et al. First reported waterborne outbreak of cryptosporidiosis with Cyclospora co-infection in Turkey. Euro Surveill. 2007;12:E070215.4. Published 2007 Feb 15.
Koturoglu G, Bayram S, Kurugol Z, et al. Frequency and risk factors of Cryptosporidium in children with acute diarrhea. T Klin J Pediatr. 2004;13:16-9.
Beyhan YE, Yılmaz H. Investigation of Cryptosporidium spp. Antigen by ELISA in stool specimens sent to our laboratory between 2010 and 2018. Turkiye Parazitol Derg. 2020;44:68-71.
Akıs FB, Beyhan YE. Distribution of intestinal parasites in patients hospitalized in child intensive care unit. Turkiye Parazitol Derg. 2018;42:113-7.
Gatei W, Wamae CN, Mbae C, et al. Cryptosporidiosis: prevalence, genotype analysis, and symptoms associated with infections in children in Kenya. Am J Trop Med Hyg. 2006;75:78-82.
Xiao L, Bern C, Limor J, et al. Identification of 5 Types of Cryptosporidium parasites in children in Lima, Peru. T J Infectious Diseases. 2001;183:492- 7.
Massoud NM, Said DE, El-Salamouny AR. Prevalence of Cyclospora cayetanensis among symptomatic and asymptomatic immune-competent children less than five years of age in Alexandria, Egypt Alex J Med. 2012;48:251-9.
Turgay N, Yolasıgmaz AU, Oyur T, et al. Monthly distribution of intestinal parasites detected in a part of Western Turkey between May 2009-April 2010-results of acid fast and modified trichrome staining methods. Turkısh J Parasitol. 2012;36:71-4.
Cicek M, Palanci Y, Ceylan A, et al. Evaluation of demographic, clinic and treatment features of patients and a cross-sectional survey of cyclosporiasis in patients with diarrhea in Southeastern Turkey. Afr J Microbiol. Res.2012;6:2949-55.
Jelinek T, Lotze M, Eichenlaub S, et al. Prevalence of infection with Cryptosporidium parvum and Cyclospora cayetanensis among international travellers. Gut. 1997;41:801-4.
Wurtz R. Cyclospora: a newly identified intestinal pathogen of humans. Clin Infect Dis. 1994;18:620-3.
Núñez FA, González OM, González I, et al. Intestinal coccidia in Cuban pediatric patients with diarrhea. Mem Inst Oswaldo Cruz. 2003;98:539-42.
Liu H, Shen Y, Yin J, et al. Prevalence and genetic characterization of Cryptosporidium, Enterocytozoon, Giardia and Cyclospora in diarrheal outpatients in China. BMC Infect Dis. 2014;25:1-6.
Guerrant DI, Moore SR, Lima AA, et al. Association of early childhood diarrhea and cryptosporidiosis with impaired physical fitness and cognitive function four-seven years later in a poor urban community in northeast Brazil. Am J Trop Med Hyg. 1999;61:707-13.