Zatiye ÇEVİKELLİ, Büşra ERTAŞ, ALİ ŞEN, Göksel ŞENER

Effect of myrtus communis extract on serum cytokines in angiotensin dependent hypertensive rats

In angiotensin dependent hypertension, high blood pressure and elevated angiotensin 2 levels lead to inflammation by increasing the pro-inflammatory cytokine levels. Since Myrtus communis have showed anti-inflammatory acvtivity in ethnobotanical researches and experimental studies, we investigated the effect of Myrtus communis extract against inflammation in a rat model of angiotensin dependent hypertension. Wistar albino rats were divided as sham-operated control, RVH and Myrtus communis extract-treated RVH groups. Left renal arteries of the rats were implanted with silver clip. Indirect blood pressure measurement of rats was provided with tail-cuff method before the surgery, 3 and 9 weeks after the surgery. Myrtus communis extract (100 mg/kg, orally) or vehicle was administered for 6 weeks. At the end of the study, serum samples were collected to investigate tumor necrosis alpha (TNF-α), interleukin-1 beta (IL-1β) and IL-6 levels. RVH resulted in significant increases in these proinflammatory cytokine levels. In the Myrtus communis extract treatment group, this increasement was abolished. The present data demonstrated that Myrtus communis attenuates RVH-induced inflammation.

PDF

___

1. Samadian F, Dalili N, Jamalian A. New insights into pathophysiology, diagnosis, and treatment of renovascular hypertension. Iranian J Kidney Diseas. 2017;11:79- 89.

2. Fliser D, Buchholz K, Haller H. Antiinﬂammatory effects of angiotensin II subtype 1 receptor blockade in hypertensive patients with microinﬂammation. Circulation. 2004;110:1103-7.

3. Schieffer B, Bünte C, Witte J, et al. Comparative effects of AT1-antagonism and angiotensin-converting enzyme inhibition on markers of inﬂammation and platelet aggregation in patients with coronary artery disease. J Am College Cardiol. 2004;44:362-8.

4. Ailawadi G, Eliason JL, Upchurch GR Jr. Current concepts in the pathogenesis of abdominal aortic aneurysm. J Vascular Surg. 2003;38:584-8.

5. Chae CU, Lee RT, Rifai N, et al. Blood pressure and inﬂammation in apparently healthy men. Hypertension. 2001;38:399-403.

6. Stumpf C, John S, Yılmaz A, et al. Enhanced levels of platelet P-selectin and circulating cytokines in young patients with mild arterial hypertension. J Hypertension. 2005;23:995-1000.

7. Sanz-Rosa, D, Oubiña MP, Cediel E, et al. Effect of AT1 receptor antagonism on vascular and circulating inﬂammatory mediators in SHR: role of NF-kappaB/IkappaB system. Am J Physiol-Heart Circulatory Physiol. 2005;288:111-5.

8. Luvara G, Pueyo ME, Philippe M, et al. Chronic blockade of NO synthase activity induces a proinﬂammatory phenotype in the arterial wall: prevention by angiotensin II antagonism. Arteriosclerosis, Thrombosis, and Vascular Biology. 1998;18:1408-141.

9. Cheng ZJ, Vapaatalo H, Mervaala E. Angiotensin II and vascular inﬂammation. Med Sci Monitor. 2005;11:194-205.

10. Suzuki Y, Ruiz-Ortega M, Lorenzo O, et al. Inflammation and angiotensin II. Int J Biochemistry Cell Biology. 2003;35:881-900.

11. Sisay M, Gashaw T. Ethnobotanical, Ethnopharmacological, and Phytochemical Studies of Myrtus communis Linn: A Popular Herb in Unani System of Medicine. J Evid Based Complementary Altern Med. 2017;22:1035-43.

12. Rossi A, Di Paola R, Mazzon E, et al. Myrtucommulone from Myrtus communis Exhibits Potent AntiInflammatory Effectiveness in Vivo. J Pharmacol Exp Ther. 2009;329:76-86.

13. Feisst C, Franke L, Appendino G, et al. Identification of molecular targets of the oligomeric nonprenylated acylphloroglucinols from Myrtus communis and their implication as anti-inflammatory compounds. J Pharmacol Exp Ther. 2005;315:389-96.

14. Goldblatt H, Lynch J, Hanzal RF, et al., Studies on Experimental Hypertension I. The production of persistent elevation of the systolic blood pressure by means of renal ischemia. J Exp Med. 1934;59:347-79.

15. Erşahin M, Sehirli O, Toklu HZ ,et al. Melatonin improves cardiovascular function and ameliorates renal, cardiac and cerebral damage in rats with renovascular hypertension. J Pineal Res. 2009;47:97-106.