Determination of Antioxidant and cytotoxic activities of king oyster mushroom mediated AgNPs synthesized with environmentally friendly methods

Synthesizing nanoparticles (NPs) using wild edible fungi with environmentally friendly synthesis methods is more preferred because of the advantages it provides. The fact that its synthesis is easy, economical, non-toxic and has a wide range of uses increases the interest in this subject. The aim of this study was to determine the antioxidant and cytotoxic activity of biomolecular synthesized Pleurotus eryngii silver nanoparticles (PE-AgNPs) against human prostate carcinoma (PC-3), human cervix (HeLa) and breast carcinoma (MCF-7) cells. PE-AgNPs showed significant cytotoxic activity against HeLa, PC-3, MCF-7 cell lines, and also dimethyl sulfoxide solvents of PE-AgNPs applied for their metal chelating activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and antioxidant activity using the β-carotene linoleate model system. Biosynthetic PE-AgNPs were found to inhibit the proliferation of PC-3, HeLa and MCF-7 cells with IC50 values of 2.185, 46.594 and 6.169 µg / ml, respectively, during a 24-hour incubation period. With the parallel of increasing concentration (1, 2, 5, 10 mg/mL) the activities were also increased at all the tests studied. At 10 mg/ml antioxidant activities were 82%, 85% and 77% for chelation of ferrous ions reducing power, DPPH scavenging and β-carotene linoleate tests respectively. The results show that PE-AgNPs may contribute to the development of a suitable anticancer drug that can lead to a new development of nanoparticles for cancer treatment. It also appears to be advantageous to use nanotechnology and green chemistry to improve the existing therapeutic properties of P.eryngii.

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1. Zhao X, Zhou L, Rajoka MSR, et al. Fungal silver nanoparticles: synthesis, application and challenges. Crit. Rev. Biotechnol. 2017;18:1–19.

2. Pastoriza-Santos I, Liz-Marzan LM.Formation and Stabilization of Silver Nanoparticles through Reduction by N,N-Dimethylformamide, Langmuir. 1999;15:948–51.

3. Pattabi M, Uchil J. Synthesis of cadmium sulphide nanoparticles. Solar Energy Mater Solar Cell. 2000;63:309–14.

4. Zhang ZQ, Patel RC, Kothari R, et al. Stable silver clusters and nanoparticles prepared in polyacrylate and inverse micellar solutions. J Phys Chem B. 2000; 104:1176–82.

5. Parikh RY, Singh S, Prasad BL, et al. Extracellular synthesis of crystalline silver nanoparticles and molecular evidence of silver resistance from Morganella sp: towards understanding biochemical synthesis mechanism. Chembiochem. 2008;9:1415–22.

6. Thakkar KN, Mhatre SS, Parikh RY. Biological synthesis of metallic nanoparticles. Nanomedicine. 2010;6:257–62.

7. Baran MF, ‘Chapter 9 1.’ Fen Bilimleri Ve Matematik Alanında Araştırma ve Değerlendirmeler .2019;110–20.

8. Philip D. Biosynthesis of Au, Ag and Au–Ag nanoparticles using edible mushroom extract. Spectrochim Acta A Mol Biomol Spectro. 2009;73:374–81.

9. Palacios I, Lozano M, Moro C, et al. Antioxidant properties of phenolic compounds occurring in edible mushrooms. Food Chem. 2011;128:674–8.

10. El-Batal AI, Al-Hazmi NE, Mosallam FM, et al. Biogenic synthesis of copper nanoparticles by natural polysaccharides and Pleurotus ostreatus fermented fenugreek using gamma rays with antioxidant and antimicrobial potential towards some wound pathogens. Microb Pathog. 2018;118:159–69.

11. Khandel P, Shahi SK. Mycogenic nanoparticles and their bio-prospective applications: current status and future challenges. J Nanostruct Chem.2018;8:369.

12. Acay H, Baran MF, Eren A. Investıgatıng antımıcrobıal actıvıty of sılver nanopartıcles produced through green synthesıs usıng leaf extract of common grape ( vıtıs vınıfera ). Appl Ecol Env Res. 2019;17:4539–46.

13. Tian J, Wong KK, Ho,CM, et al. Topical delivery of silver nanoparticles promotes wound healing. Med Chem. 2007;2:129–36.

14. Nagajyothi PC, Sreekanth TVM, Lee J, et al. Mycosynthesis: antibacterial antioxidant and antiploriferative activities of silver nanoparticles synthesized from Inonotus obliquus (Chaga mushroom) extract. J Photochem Photobiool. 2014;130:299–304.

15. Lukman AI, Gong B, Marjo CE, et al. Facile synthesis, stabilization, and antibacterial performance of discrete Ag nanoparticles using Medicago sativa seed exudates. J Coll Interface Sci. 2011;353:433–44.

16. Miller KD, Siegel RL, Lin CC, et al. Cancer treatment and survivorship statistics. A Cancer J. Clinicians. 2016;46:231–9.

17. Kim DW, Hong GH, Lee HH, et al. Effect of colloidal silver against the cytotoxicity of hydrogen peroxide and naphthazarin on primary cultured cortical astrocytes. Neurosci. 2007;117:387-400.

18. Lupu R, Cardillo M, Cho C, et al. The significance of heregulin in breast cancer tumor progression and drug resistance. Breast Cancer Res Treat. 1996;38:57–66.

19. Johnston SR. Acquired tamoxifen resistance in human breast cancer – potential mechanisms and clinical implications. Anticancer Drugs. 1997;8:911–30.

20. Brown K. Breast cancer chemoprevention: risk-benefit effects of the antioestrogen tamoxifen. Expert Opin Drug Saf. 2002;1:253–67.

21. Kulkarni RR, Shaiwale NS, Deobagkar DN, et al. Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity. Int J Nanomed. 2015;10:963–74.

22. Devanesan S, AlSalhi MS, Vishnubalaji R, et al. Rapid biological synthesis of silver nanoparticles using plant seed extracts and their cytotoxicity on colorectal cancer cell lines. J Clust Sci. 2017;28:595–605.

23. Nakkala JR, Mata R, Sadras SR, Green synthesized nano silver: synthesis, physicochemical profiling, antibacterial, anticancer activities and biological in vivo toxicity. J Colloid Interface Sci. 2017;499:33–45.

24. Han JW, Gurunathan S, Jeong JK, et al. Oxidative stress mediated cytotoxicity of biologically synthesized silver nanoparticles in human lung epithelial. Nanoscale Res Lett. 2014;9:459–73.

25. Acay H, Baran MF. Biosynthesis and characterization of silver nanoparticles using king oyster (pleurotus eryngii) extract: effect on some microorganisms. Appl Ecol Env Res. 2019;17:4539–46.

26. Baran MF. Synthesıs, Characterızatıon and investıgatıon of antımıcrobıal actıvıty of sılver nanopartıcles from cydonıa oblonga leaf. Appl Ecol Env Res. 2019;17:2583–92.

27. Alley MC, Scudiero DA, Monks A et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 1988;48:589-601.

28. Hatano T, Kagawa H, Yasuhara T, et al. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chem Pharm Bull. 1988;36:2090–7.

29. Mi-Yae S, Tae-Hun K, Nak-Ju S. Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food Chem. 2003;82:593–7.

30. Dinis TCP, Madeira VMC, Almeida LM. Action of phenolic derivates (acetoaminophen, salicylate and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys.1994;315:161−9.

31. Yehia RS, Al-Sheikh H. Biosynthesis and characterization of silver nanoparticles produced by pleurotus ostreatus and their anticandidal and anticancer activities. World J. Microbiol. Biotechnol. 2014;30:2797–803.

32. Gurunathan S, Han JW, Kim JH. Green chemistry approach for the synthesis of biocompatible graphene. Int. J. Nanomed. 2013;8:2719–32.

33. Prabakaran K, Ragavendran C, Natarajan,D, Mycosynthesis of silver nanoparticles from Beauveria bassiana and its larvicidal, antibacterial, and cytotoxic effect on human cervical cancer (HeLa) cells. RSC Adv. 2016;6:44972.

34. Manivasagan P, Venkatesan J, Sivakumar K, et al. Marineactinobacterial metabolites: current status and future perspectives. Microbiol Res. 2013;168:311- 32.

35. Rathod D, Golinska P, Wypij M. et al. A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity. Med Microbiol Immunol. 2016;205:435.

36. Barabadi H, Muhammad Ovais M, Shinwari ZK, et al. Anti-cancer green bionanomaterials: present status and future prospects. Green Chem Lett Rev. 2017;10:285-314.

37. Ferlay J, Shin H, Bray F, et al. Cancer Incidence and Mortality Worldwide: IARC Cancer Base No. 10.GLOBOCAN 2008, International Agency for Research on Cancer, Lyon. 2010.

38. Raman J, Reddy GR, Lakshmanan H, et al. Mycosynthesis and characterization of silver nanoparticles from pleurotus djamor var. roseus and their in vitro cytotoxicity effect on PC3 cells. Process Biochem. 2015;50:140–7.

39. Sanpui P, Chattopadhyay A, Ghosh SS, Induction of apoptosis in cancer cells at low silver nanoparticle concentrations using chitosan nanocarrier. ACS Appl Mater Interfaces. 2011;3:218–28.

40. Keshari AK, Srivastava R, Singh P, et al. Antioxidant and antibacterial activity of silver nanoparticles synthesized by Cestrum nocturnum. J Ayurveda Integr. Med. 2018;1-8.