Badel ARSLAN, Nurcan ARAS, Gül YAŞ, Ayşegül ÇETİNKAYA

1800MHz Radiofrequency electromagnetic radiation: Does it affect heat shock genes expression levels in the rat brain?

The brain could recognizethe cell phone radiation as environmental stress and may alter stress-related gene expression levels. Heat shock proteins (HSP) are a family of proteins that are produced by cells in response to the exposure of stressful conditions. We investigated whether exposure to 1800MHz Radiofrequency Electromagnetic Radiation (RF-EMR) recognize the expression levels of some HSPs (Cryaa, Crybb, Hsp20, Hsp25, Hsp70) in mature rat’s brain tissue. The experimental group was exposed to 1.800MHz RF-EMR in restrainer for eight weeks with 2 h/day. The specific absorption rate (SAR) was 0.06 W/kg. The sham group was kept in restrainer but not exposed to radiation. The Control group was kept under their condition. Whole brain was homogenized.ViA7 Real-Time-PCR software was used to measure CT values of the genes and ΔΔCT values were also calculated. Cryaa (p=0.02), Crybb (p=0.01), and Hsp20 (p

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1. Xue-Sen Y, Gen-Lin H, Yu-Tong H, et al. Exposure to 2.45 GHz electromagnetic fields elicits an HSP-related stress response in rat hippocampus. Brain Res Bulletin. 2012;88:371– 8.

2. Shaymaa H, Abdel-Aleem ES. Mona KG. Biochemical and histological studies on adverse effects of mobile phone radiation on rat’s brain. J Chem Neuro. 2016;78:10–9.

3. Mehran Z, Alireza K, Javad S, et al. the impact of using cell phones after lightout on sleep quality, headache, tiredness, and distractibility among students of a University in North of Iran. Iranian J Psych Behav Sci. 2015;9:2010.

4. Hamblin DL, Wood AW. Effects of mobile phone emissions on human brain activity and sleep variables. Int J Radiat Biol. 2002;78:659–69.

5. Nittby H, Grafström G, Tian DP, et al. Cognitive impairment in rats after longterm exposure to GSM-900 mobile phone radiation. Bioelectromagnetics. 2008; 29 (3): 219–232.

6. Kesari K K, Kumar S, Behari J. 900-MHz microwave radiation promotes oxidation in rat brain. Electromagnet Biol Med. 2011;30:219–34.

7. Kesari KK, Behari J, Kumar S. Mutagenic response of 2.45 GHz radiation exposure on rat brain. Int J Radiat Biol. 2010;86:334–43.

8. Kesari KK, Meena R, Nirala J, et al. Effect of 3G cell phone exposure with computer controlled 2-D stepper motor on non-thermal activation of the Hsp27/p38MAPK stress pathway in rat brain. Cell Bioc Biop. 2014;68:347– 58.

9. Carra S, Alberti S, Arrigo PA, et al. The growing world of small heat shock proteins: from structure to functions. Cell Stress Chaperon. 2017;22:601–11.

10. Gaestel M. Biological monitoring of non-thermal effects of mobile phone radiation: recent approaches and challenges. Biol Rev. 2010;85:489–500.

11. Brown IR. Heat shock proteins and protection of the nervous system. Ann N Y Acad Sci. 2007;1113:147–58.

12. Quraishe S, Asuni A, Boelens W C, et al. Expression of the small heat shock protein family in the mouse CNS: differential anatomical and biochemical compartmentalization. Neurosci. 2008;153:483–91.

13. Balakrishnan K, Murali V, Rathika C, et al. Hsp70 is an independent stress marker among frequent users of mobile phones. J Environ Pathol Toxicol Oncol. 2014;33:339-47.

14. Calabrò E, Condello S, Currò M, et al. Modulation of heat shock protein response in SH-SY5Y by mobile phone microwaves. World J Biolog Chem. 2012;3:34-40.

15. Lantow M, Schuderer J, Hartwig C, et al. free radical release and HSP70 expression in two human immune-relevant cell lines after exposure to 1800 MHz radiofrequency radiation. Radiat Res. 2006;165:88-94.

16. Lantow M, Lupke M, Frahm J, et al. ROS release and Hsp70 expression after exposure to 1,800 MHz radiofrequency electromagnetic fields in primary human monocytes and lymphocytes. Radiat Environ Biophys. 2006;45:55– 62.

17. Lee YJ, Lee DH, Cho CK, et al. HSP25 inhibits radiation-induced apoptosis through reduction of PKCd-mediated ROS production. Oncogene. 2005;24:3715–25.

18. Sanchez S, Masuda H, Ruffié G, et al. Effect of GSM-900 and -1800 signals on the skin of hairless rats. III: Expression of heat shock proteins. Int J Radiat Biol. 2008;84:61-8.

19. Horwitz J. Alpha-crystallin. Experimental Eye Res. 2003;76:145–53. 20. Wistow G. The human crystallin gene families. Wistow Human Genomics. 2012;6:26.

21. Tikhomirova T S, Selivanova O M, Galzitskaya O V. α_Crystallins are small heat shock proteins: functional and structural properties. Biochemistry. 2017; Moscow Vol. 82 No. 2.

22. Micheal F, Festing W. Reduction of animal use: experimental design and quality of experiments. Laboratory Animals. 1994;28:212-21.

23. Nisbet HO, Akar A, Nisbet C, et al. Effects of electromagnetic field (1.8/0.9 GHz) exposure on growth plate in growing rats. Res Veterinar Sci. 2016;104:24-9.

24. French PW, Penny R, Laurence JA, et al. Mobile phones, heat shock proteins and cancer. Differentiation. 2000;67:93–7.

25. Belyaev I. Non-thermal biological effects of microwaves. Microwave Rev. November 2005.

26. Bagnéris C, Bateman O A, Naylor C E, et al. Crystal structures of α-Crystallin Domain Dimers of αB-Crystallin and Hsp20. J Molecular Biology. 2009;392:1242–52.

27. M Lantow, M Lupke, J Frahm, et al. ROS release and Hsp70 expression after exposure to 1,800 MHz radiofrequency electromagnetic fields in primary human monocytes and lymphocytes. Radiation Environmental Biophysics. 2006;45:55–62.

28. Weisbrot D, Lin H, Ye L, et al. Effects of mobile phone radiation on reproduction and development in drosophila melanogaster. J Cellular Biochemis. 2003;89:48–55.

29. Hussein S, El-Saba AA, Galal MK. Biochemical and histological studies on adverse effects of mobile phone radiation on rat’s brain. J Chemic Neuroanatom. 2016;78:10–9.

30. Dasdag S, Akdag MZ, Ulukaya E, et al. Effect of mobile phone exposure on apoptotic glial cells and status of oxidative stress in rat brain. Electromagnetic Biology Med. 2009;28:342-54.

31. Miyakoshi J, Takemasa K, Takashima Y, et al. Effects of exposure to a 1950 MHz radio frequency field on expression of Hsp70 and Hsp27 in human glioma cells. Bio Electro Magnetics. 2005;26:251-7.

32. Leszczynski D, Joenväärä S, Reivinen J, et al. Non-thermal activation of the HSP27/P38MAPK stress pathway by mobile phone radiation in human endothelial cells: molecular mechanism for cancer and blood-brain barrierrelated effects. Differentiation. 2002;70:120–9.

33. Dasdag S, Akdag MZ, Erdal ME, et al. Effects of 2.4 GHz radiofrequency radiation emitted from Wi-Fi equipment on microRNA expression in brain tissue. Int J Radiation Biology. 2015;91:555–61.

34. Dasdag S, Akdag MZ, Erdal ME, et al. Long term and excessive use of 900 MHz radiofrequency radiation alter micro RNA expression in brain. Int J Radiatio Biolog. 2015;91:306–11.

35. Graw J. From eyeless to neurological diseases. Experiment Eye Res. 2017;156;5-9.

36. Spadaro P A, Flavell C R, Widagdo J, et al. Long noncoding RNA-directed epigenetic regulation of gene expression is associated with anxiety-like behavior in mice. Biological Psychiatry. 2015;78:848-59.

37. Guptea A A, Morrisa J K, Zhanga H, et al. Age-related changes in HSP25 expression in basal ganglia and cortex of F344/BN rats. Neuroscience Letters. 2010;472:90–3.

38. Sharp FR, Kinouchi H, Koistinaho J, et al. HSP70 heat shock gene regulation during ischemia. Stroke.1993;24:172-5.