Celal ALANDAĞ, Ulvi DEMİREL, Kürşat KARGÜN

Kronik Hepatit B Enfeksiyonu ile İnterlökin-28B Gen Polimorfizminin İlişkisi

Amaç: İnterlökin-28B (IL28B) geni polimorfizmi hepatit B virus (HBV) enfeksiyonunun prognozunda önemli olabileceğine dair yayınlar bulunmaktadır. Bu çalışmada IL28B geni ile HBV enfeksiyonunun viral klirens ve kronik HBV enfeksiyonunda viral yük arasındaki ilişkiyi araştırmayı planladık.Materyal ve Metot: Ekim 2011 ile Ekim 2012 tarihleri arasında merkezimize başvuran 146 kişi çalışmaya dahil edildi. Kronik hepatit B (KHB) grubunda 117, kontrol grubunda 29 kişi vardı. Tüm gruplarda IL28B geni rs12979860, rs12980275 polimorfizmlerine ve IL28B geni ekspresyon düzeylerine (mRNA) bakıldı ve gruplar karşılaştırıldı.Bulgular: Rs12979860 gen polymorfizmi oranları açısından gruplar arasında fark yoktu (χ2=0.36, p=0.835). Ama rs12980275 bölgesi açısından KHB grubunda A/A genotipi daha fazla iken (χ2=55.2, p<0.001), G/A ve G/G genotipleri istatiksel anlamlı olarak daha az bulundu. Gruplar arasında MRNA düzeyleri açısından fark yoktuSonuç: Öncelikle bu çalışma bölgemizdeki IL28 gen polimorfizm oranlarını ortaya koyan ilk çalışma olmasından dolayı önemlidir. KHB ile rs12980275 polimorfizmi arasındaki ilişkinin klinik önemi bulunduğu düşünülmektedir. İleride bu konuda yapılacak çalışmalara ışık tutmaktadır.

Anahtar Kelimeler:

İL28 B polimorfizmi, İL28B ekspresyonu, kronik hepatit B, Türkiye

Relationship Between Interleukin-28B Gene Polymorphism and Chronic Hepatitis B Infection

Aim: Interleukin 28B (IL28B) gene polymorphism may play a role in hepatitis B virus (HBV) infection prognosis. We investigated the effects of IL28B gene polymorphism on viral clearance and viral load in chronic hepatitis B (CHB) patients.Material and Methods: We included 146 individuals who applied to our center between October 2011 and October 2012. CHB (N=117) and control (N=29) groups were compared in terms of IL28B gene rs12979860, rs12980275 gene region polymorphisms, and IL28B gene expression (mRNA) levels.Results: There was no statistically significant difference between groups in terms of rs12979860 gene region polymorphism rates (χ2=0.36, p=0.835). But there was a significant association between groups in terms of rs12980275. In the CHB group, the A/A genotype was much more, (χ2=55.2, p<0.001), G/A and G/G genotypes were less frequent. There was no statistically significant difference between the groups in terms of IL28B expression levels.Conclusion: This study revealed the genotype profile of the IL28B gene of our region. It is the first study on this subject in our region. The association between CHB and rs12980275 polymorphism may be important. Our results will contribute to future studies.

Keywords:

Chronic hepatitis B, IL 28B polymorphism, IL 28B expression, Turkey,

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