Pentilenetetrazol ile Oluşturulan Deneysel Epilepsi Modelinde Farklı Dozlardaki Melatoninin Etkilerinin İncelenmesi

Amaç: Bu çalışmada, pentilenetetrazol (PTZ) ile indüklenen deneysel epilepsi oluşturulmuş sıçanlarda melatonin (MEL) tedavisinin etkisinin değerlendirilmesi amaçlanmıştır. Kontrol grubu, epilepsi grubu ve iki tedavi grubundaki değişiklikler (25 mg/kg ile 100 mg/kg) grup içi ve gruplar arası olarak değerlendirilmiştir. Gereç ve Yöntemler: Kırk adet erkek Wistar albino sıçan (12-14 haftalık) 4 farklı gruba (kontrol, PTZ, MEL25, MEL100) ayrıldı. Kontrol grubuna sadece çözgen enjekte edildi ve PTZ grubuna 35 mg/kg dozunda PTZ intraperitoneal toplam 23 gün süresince 12 kez günaşırı uygulandı. Melatonin, MEL25 grubu için 25 mg/kg ve MEL100 grubu için 100 mg/kg uygulandı. Deney sırasında ve sonrasında test edilenparametreler davranış testi (yükseltilmiş artı labirent), beyin dokusunda biyokimyasal testler [dekapitasyon sonrası; malondialdehit (MDA), katalaz (CAT), süperoksit dismutaz (SOD), toplam antioksidan durumu (TAS) ve toplam oksidan durumu (TOS)] ve epileptik nöbet şiddeti ölçek skorları (her enjeksiyon günü) idi. Bulgular: Epilepsi grubu ile kontrol ve tedavi grupları arasında kaçış ve saklanma süreleri, nöbet skorları ve biyokimyasal testler açısından anlamlı farklılıklar bulundu (p

Effects of Melatonin at Different Doses on Experimental Epilepsy Model Induced By Pentylenetetrazole

Objective: This study aimed to evaluate the effect of melatonin (MEL) treatmenton rats with experimental epilepsy induced by pentylenetetrazole (PTZ). Changesin the control, epilepsy and two treatment groups (25 mg/kg and 100 mg/kg) weremonitored as intragroup and intergroup changes.Materials and Methods: Forty male Wistar albino rats (12-14 weeks old) weredivided into control, PTZ, MEL25 and MEL100 groups, with 10 rats in each group.Only solvent was injected in the control group, and PTZ at a dose of 35 mg/kg wasadministered intraperitoneal 12 times in a total of 23 days in the PTZ group. MEL25and 100 mg/kg were administered in the MEL25 and MEL100 groups, respectively.Parameters tested during and after the experiment were behavioural tests (elevatedplus maze), biochemical tests in brain tissue [after decapitation; malondialdehyde(MDA), catalase (CAT), superoxide dismutase (SOD), total antioxidant status (TAS)and total oxidant status (TOS)] and epileptic seizure severity scale scores (everyinjection day).Results: Significant differences were observed among the epilepsy, control andtreatment groups, except for the hiding times, seizure scores and biochemicaltests (p

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