Ayse Nilhan AKSU, Tayfun BILGIC, Cavit Kerem KAYHAN, Zumrut Mine ISIK SAGLAM, Nazli CAF

Partial healing effects of St. John’s wort oil on the rat excisional wound model

Objective: St. John’s wort (SJW) oil (Hypericum perforatum) has been used for its immunomodulatory and anti-inflammatory effects. Several studies have shown the efficacy of SJW on wound healing. The aim of this study is to assess the effectiveness of SJW using a combination of biochemical, histopathological and laser Doppler evaluations. Materials and Methods: Sixteen young Wistar albino rats were used as case and control groups (having 8 in each group). After anesthesia protocol, 6 mm punch biopsy was taken from six separate sites on the rats’ dorsal skin. Three wounds were stitched (closed wounds); three wounds were left as they were (open wounds). SJW oil was administered topically to case group once a day for 14 days. Controls did not receive any treatment. Results: There was no statistical difference in blood perfusion between the groups. No statistical difference was present between the groups in GPx (glutathione peroxidase)values. Rat MDA (malonyldialdehyde) values were higher in the case group compared to the control group. SJW oil was found to be beneficial and effective within some histological parameters. Conclusion: SJW may be an effective salve within some parameters. Nevertheless, this judgment is uncertain due to the low sample size. We encourage further studies on this promising natural medicine.

Keywords:

Antiinflammatory, fibroblast, glutathione peroxidase, St. John’s wort, wound,

PDF

___

Belwal T, Devkota HP, Singh MK, et al. St. John’s Wort (Hypericum Perforatum) in Nonvitamin and Nonmineral Nutrirional Supplements. Nabavi SM, Silva AS. Elsevier Inc.; 2019. Chapter 3.40. https://doi.org/10.1016/C2016-0-03546-5
Klemow KM, Bartlow A, Crawford J, Kocher N, Shah J, Ritsick M. Medical Attributes of St. John’s Wort (Hypericum perforatum). In: Benzie IFF, Wachtel-Galor S, editors. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd ed. Boca Raton (FL): CRC Press/Taylor & Francis; 2011. Chapter 11. PMID: 22593920
Barrientos S, Brem H, Stojadinovic O, et al. Clinical application of growth factors and cytokines in wound healing. Wound Repair Regen 2014;22:5:569-78. doi:10.1111/wrr.12205
Topal Hançer A, Yılmaz Pınar. Editöre mektup: Balın Yara İyileşmesi Üzerine Etkisinin Değerlendirilmesi. Bozok Tıp Derg 2019;9:1:152-9. doi:10.16919/bozoktip.399768
Rodrigues M, Kosaric N, Bonham CA, et al. Wound healing: A cellular perspective. Physiol Rev 2019;99:1:665-706. doi:10.1152/physrev.00067.2017
Gunpinar S, Kilic OA, Duran I, et al. Tosun M, Firat T, Soyler G. Evaluation of the Effect of Topical Hypericum perforatum Oil on Excisional Palatal Wound Healing in Rabbits. J Investig Surg 2020;33:1:49-58. doi:10.1080/08941.939.2018.1474980
Abd Jalil MA, Kasmuri AR, Hadi H. Stingless bee honey, the natural wound healer: A review. Skin Pharmacol Physiol 2017;30:2:66-75. doi:10.1159/000458416
Trautinger F, Schwarz T, Luger T. Cytokines in dermatotherapy. J Dermatolog Treat 1992;3:3:143-152. doi:10.3109/095.466.39209088710
Morelli T, Neiva R, Nevins ML, et al. Angiogenic biomarkers and healing of living cellular constructs J Dent Res. 2011;90:4:456-62. doi:10.1177/002.203.4510389334
Murthy S, Gautam MK, Goel S, et al. Evaluation of in vivo wound healing activity of Bacopa monniera on different wound model in rats. Biomed Res Int 2013;2013. doi:10.1155/2013/972028
Yücel A, Kan Y, Yesilada E, Akın O. Effect of St.John’s wort (Hypericum perforatum) oily extract for the care and treatment of pressure sores; a case report. J Ethnopharmacol 2017;196:236-41. doi:10.1016/j.jep.2016.12.030
Grundmann O, Lv Y, Kelber O, et al. Mechanism of St. John’s wort extract (STW3-VI) during chronic restraint stress is mediated by the interrelationship of the immune, oxidative defense, and neuroendocrine system. Neuropharmacology 2010;58:4-5:767-73. doi:10.1016/j.neuropharm.2009.12.014
Fiebich BL, Höllig A, Lieb K. Inhibition of substance P-induced cytokine synthesis by St. John’s wort extracts. Pharmacopsychiatry 2001;34: 1:26-8. doi:10.1055/s-2001-15462
DeRose V, Robbins R, Snider R, et al. Substance P increases neutrophil adhesion to bronchial epithelial cells. J Immunol 1994;152:3:1339–46
Lu YH, Du C Bin, Liu JW, et al. Neuroprotective effects of Hypericum perforatum on trauma induced by hydrogen peroxide in PC12 cells. Am J Chin Med 2004;32:3:397-405. doi:10.1142/S0192415X04002053
Silva B, Oliveira PJ, Dias A, et al. Quercetin, kaempferol and biapigenin from hypericum perforatum are neuroprotective against excitotoxic insults. Neurotox Res 2008;13:3-4:265-79. doi:10.1007/BF03033510
Zou Y, Lu Y, Wei D. Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro. J Agric Food Chem 2004;52:16:5032-39. doi:10.1021/jf049571r
Sánchez-Reus MI, Gómez del Rio MA, Iglesias I, et al. Standardized Hypericum perforatum reduces oxidative stress and increases gene expression of antioxidant enzymes on rotenone-exposed rats. Neuropharmacology 2007;52:2:606- 16.doi:10.1016/j.neuropharm.2006.09.003
Han MC, Durmuş AS, Sağliyan A, et al. Effects of Nigella sativa and Hypericum perforatum on wound healing. Turkish J Vet Anim Sci 2017;41:1:99-105. doi:10.3906/vet-1603-26
Bilgen F, Ural A, Kurutas EB, Bekerecioglu M. The effect of oxidative stress and Raftlin levels on wound healing. Int Wound J 2019;16:5:1178-84. doi:10.1111/iwj.13177

ISSN: 1019-1941
Yayın Aralığı: Yılda 3 Sayı
Başlangıç: 1988
Yayıncı: Marmara Üniversitesi

Arşiv