Time-dependent change of the digestive enzyme activity of Black Sea salmon (Salmo labrax Pallas, 1814) fed at suboptimal temperature

The present study conducted at 10.21±0.27°C water temperature in freshwater recirculating aquaculture systems (RAS) to observe the temporal variations in the digestive enzyme activities including pepsin, trypsin, amylase and lipase of Black Sea salmon (Salmo labrax). Seventh filial generation (F7) of Black Sea salmon (Salmo labrax) with average initial weights of 69.85±10.08 g were by hand fed three times daily until apparent satiation. At the end of the 75-day trial the samples were dissected that reached a weight of 179.17±31.08 g at 45th minute, 3rd, 6th, 12th, 24th, 36th, 48th, 72nd and 96nd hours post feeding. In all enzyme groups, the third hour after feeding was recorded as the time when the highest levels were observed. However, enzyme activities decreased gradually as the time after feeding was prolonged. In the nutrition studies to be conducted at a suboptimal temperature in RAS, gut sampling of Black Sea salmon can be taken at 3rd hour after feeding. For a better understanding of digestive enzyme activity for this species, however, different sections of the digestive system should be comprehensively monitored including different temperature conditions.

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