Electrochemical biosensor for simultaneously detection of Tamoxifen
Electrochemical biosensor for simultaneously detection of Tamoxifen
Cancer is described as the uncontrollably multiplying abnormal proliferation of cells. Cancer can affect everyone, and risk of which rises with age, lifestyle, and environmental toxins. Tamoxi̇fen (TAM) which is a selective estrogen receptor modulator, has estrogenic or antiestrogenic effects on the breast tissue by binding to the estrogen receptors. The current study presents a voltammetric biosensor to identify the effect of Tamoxifen on DNA structure. In this study, the effect of TAM on the double-stranded DNA (dsDNA) was investigated electrochemically in both the presence and absence of antioxidants. For this purpose, TAM-dsDNA-antioxidant interaction investigated by using the pencil graphite electrode (PGE). The DNA modified sensor was created simply by wet-adsorbtion method. The prepared biosensor was examined electrochemically by square wave voltammety (SWV) method, and its lowest concentration and optimum pH range were determined. The effect of TAM on dsDNA was investigated simultaneously for the first time in the literature.
___
- [1]. M. Clemons, S. Danson, and A. Howell, “Tamoxifen ('Nolvadex’): A review,” Cancer Treat. Rev., vol. 28, no. 4,
pp. 165–180, 2002, doi: 10.1016/S0305-7372(02)00036-1.
- [2]. D. J. Bentrem and V. Craig Jordan, “Tamoxifen, raloxifene and the prevention of breast cancer.,” Minerva
Endocrinol., vol. 27, no. 2, pp. 127–139, 2002, doi: 10.1210/edrv.20.3.0368.
- [3]. S. Mandlekar and A. N. T. Kong, “Mechanisms of tamoxifen-induced apoptosis,” Apoptosis, vol. 6, no. 6, pp.
469–477, 2001, doi: 10.1023/A:1012437607881.
- [4]. M. Fouladgar, H. Karimi-Maleh, F. Opoku, and P. P. Govender, “Electrochemical anticancer drug sensor for
determination of raloxifene in the presence of tamoxifen using graphene-CuO-polypyrrole nanocomposite
structure modified pencil graphite electrode: Theoretical and experimental investigation,” J. Mol. Liq., vol. 311, p.
113314, 2020, doi: 10.1016/j.molliq.2020.113314.
- [5]. I. Girault, I. Bièche, and R. Lidereau, “Role of estrogen receptor α transcriptional coregulators in tamoxifen
resistance in breast cancer,” Maturitas, vol. 54, no. 4, pp. 342–351, 2006, doi: 10.1016/j.maturitas.2006.06.003.
- [6]. M. M. T. Buckley and K. L. Goa, “Tamoxifen: A Reappraisal of its Pharmacodynamic and Pharmacokinetic
Properties, and Therapeutic Use,” Drugs, vol. 37, no. 4, pp. 451–490, 1989, doi: 10.2165/00003495-198937040-
00004.
- [7]. B. J. A. Furr and V. C. Jordan, “The pharmacology and clinical uses of tamoxifen,” Pharmacol. Ther., vol. 25,
no. 2, pp. 127–205, 1984, doi: 10.1016/0163-7258(84)90043-3.
- [8]. K. Lee, B. A. Ward, Z. Desta, D. A. Flockhart, and D. R. Jones, “Q uantification of tamoxifen and three
metabolites in plasma by high-performance liquid chromatography with fluorescence detection : application
to a clinical trial,” vol. 791, pp. 245–253, 2003.
- [9]. X. Liu, J. Zhang, J. Yin, and H. Duan, “Analysis of hormone antagonists in clinical and municipal wastewater
by isotopic dilution liquid chromatography tandem mass spectrometry,” pp. 2977–2985, 2010, doi:
10.1007/s00216-010-3531-0.
- [10]. S. Xiao, Y. Yang, J. Zhang, Y. Wu, and B. Shao, “[Determination of 6 antiestrogens in fish tissues by ultra
performance liquid chromatography-tandem mass spectrometry],” Se pu = Chinese J. Chromatogr., vol. 29,
no. 11, p. 1055—1061, 2011, [Online]. Available: http://europepmc.org/abstract/MED/22393691.
- [11]. K. Radhapyari, P. Kotoky, and R. Khan, “Detection of anticancer drug tamoxifen using biosensor based on
polyaniline probe modified with horseradish peroxidase,” Mater. Sci. Eng. C, vol. 33, no. 2, pp. 583–587, 2013,
doi: 10.1016/j.msec.2012.09.021.
- [12]. S. Yanik, D. Ozkan-ariksoysal, and S. Yilmaz, “ELECTROCHEMICAL BIOSENSOR FOR BRCA1 GENE AND
TAMOXIFEN INTERACTION,” vol. 4, no. 1, pp. 35–48, 2020.
- [13]. L. Dornelles, S. Hernandez, G. Marrazza, M. Mascini, and L. Tatsuo, “Investigations of the antioxidant
properties of plant extracts using a DNA-electrochemical biosensor,” vol. 21, pp. 1374–1382, 2006, doi:
10.1016/j.bios.2005.05.012.
- [14]. E. Küpeli Akkol, I. Süntar, M. Ilhan, and E. Aras, “In vitro enzyme inhibitory effects of Rubus sanctus
Schreber and its active metabolite as a function of wound healing activity,” J. Herb. Med., 2015, doi:
10.1016/j.hermed.2015.09.002.
- [15]. I. Erlund et al., “Pharmacokinetics of quercetin from quercetin aglycone and rutin in healthy
volunteers,” Eur. J. Clin. Pharmacol., vol. 56, no. 8, pp. 545–553, 2000, doi: 10.1007/s002280000197.
- [16]. S. Allahverdiyeva, O. Yunusoğlu, Y. Yardım, and Z. Şentürk, “First electrochemical evaluation of
favipiravir used as an antiviral option in the treatment of COVID-19: A study of its enhanced voltammetric
determination in cationic surfactant media using a boron-doped diamond electrode,” Anal. Chim. Acta, vol.
1159, 2021, doi: 10.1016/j.aca.2021.338418.
- [17]. H. Subak and D. Ozkan-Ariksoysal, “Label-free electrochemical biosensor for the detection of Influenza
genes and the solution of guanine-based displaying problem of DNA hybridization,” Sensors Actuators, B
Chem., vol. 263, 2018, doi: 10.1016/j.snb.2018.02.089