UV-C’nin, Deinonoccus radiodurans ve Vitreoscilla Hemoglobin (vgb) Geni Aktarılmış Rekombinantlarında; SOD, KAT ve Karoten Miktarı Üzerine Etkisi

Ultraviyole radyasyon (UV); biyolojik dokularda reaktif oksijen türlerinin meydana gelmesine neden olarak oksidatif stres oluşturmaktadır. UV’ nin indüklediği reaktif oksijen türleri, bunların etkileri ve bunlara karşı hücresel savunma mekanizmaları ve reaktif oksijen türlerinin temizlenmesinden sorumlu antioksidan sistemleri günümüzde üzerinde oldukça fazla araştırma yapılan konulardır. Bu çalışmada, yüksek seviyede iyonize radyasyon ve UV radyasyon, kuraklık ve DNA’ ya zarar veren kimyasallar gibi birçok ajan ve koşula olan direnciyle iyi bilinen bir ekstremofil olan Deinococcus radiodrans ile Vitreoscilla hemoglobin (vgb) geni klonlanmış rekombinantı ve kontrol olarak da vgb ̄ rekombinant suşu kullanılmıştır. UV-C’ nin D. radiodurans' ın antioksidan savunma sistemleri (süperoksit dismutaz, katalaz ve karoten) üzerine etkisi araştırılıp, buna ek olarak organizmaya daha fazla oksijenli ortam sağlayarak daha fazla büyümesini sağlayan vgb geninin, bakterinin UV direncine yapacağı katkısı araştırılmıştır. Buna göre, D. radiodurans (vgb ̄ )' in UV-C uygulanan örnekleri kontrol gruplarıyla kıyaslandığında süperoksit dismutaz ve katalaz enzim aktivitesinin yabanıl ve vgb genini taşıyan rekombinantına oranla daha düşük olduğu tespit edilmiştir. Yine yüksek karoten içeren yabanıl tipi bakterilerde, UV-C uygulamasına bağlı olarak karoten miktar artışı net bir şekilde gözlenmiştir.

In UV-C, Deinonoccus radiodurans and Vitreoscilla Hemoglobin (vgb) Gene Transferred Recombinants; Effect on SOD, KAT and Carotene Amount

Ultraviolet radiation (UV); creates oxidative stress by causing the formation of reactive oxygen species in biological tissues. Reactive oxygen species induced by UV, their effects and cellular defense mechanisms against them, and antioxidant systems responsible for cleaning reactive oxygen species are the subjects of much research today. In this study, Deinococcus radiodrans which is well known an extremophile for its resistance to many agents and conditions such as high levels of ionizing radiation and UV radiation, drought and chemicals that damage DNA and Vitreoscilla hemoglobin (vgb) gene cloned recombinant with, and vgb ̄ recombinant strain as a control were used. The effect of UV-C on the antioxidant defense systems of D. radiodurans (superoxide dismutase, catalase and carotene) was investigated, and in addition, the contribution of the vgb gene, which provides more oxygenated environment to the organism, to the UV resistance of the bacteria, was investigated. Accordingly, when UV-C treated samples of D. radiodurans (vgb ̄) were compared with the control groups, it was determined that the superoxide dismutase and catalase enzyme activities were lower than the wild and the recombinant carrying the vgb gene. Again, in wild-type bacteria with high carotene, an increase in the amount of carotene was clearly observed due to UV-C application.

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