Mehmet BAŞHAN, Mehmet Cemal TALAY, Vedat KARACA

Süne, Eurygaster integriceps’in Put. (Heteroptera: Scutelleridae) Diyapoz Öncesi ve Diyapoz Sonrası Erginlerinden Hazırlanan Fosfolipit Altsınıflarının Yağ Asidi Bileşiminindeki Değişiklikler

Bu çalışmada, süne, Eurygaster integriceps’in diyapoz öncesi ergin vediyapoz sonrası erginlerinin fosfatidilkolin (PC), fosfatidiletanolamin(PE), fosfatidilinositol (PI) ve fosfatidilserin (PS) gibi fosfolipit (PL)altsınıflarının yağ asidi kompozisyonundaki değişiklikleraraştırılmıştır. Fosfolipit alt sınıflarının ayrılmasında İnce TabakaKromatoğrafisi tekniği, yağ asitlerinin analizinde ise Gazkromatoğrafi cihazı kullanılmıştır. Eurygaster integriceps’in PLaltsınıflarında gözlenen dominant yağ asitleri, doymuş yağasitlerinden (SFA) palmitik asit (16:0), tekli doymamış yağasitlerinden (MUFA) oleik asit (18:1n-9) ve çoklu doymamış yağasitlerinden (PUFA) linoleik asit (18:2n-6) idi. Diyapoz öncesi vediyapoz sonrası erginlerin PC, PE ve PS fraksiyonlarının yağ asidiiçeriğindeki değişikliklerin benzer olduğu bulunmuştur. Diyapozsonrası erginlerin bu fraksiyonlarında 16:0, 18:0 ve ∑SFA yüzdeleridiyapoz öncesi erginlere oranla daha düşük; ancak16:1n-7, ∑MUFA,18:2n-6 ve ∑PUFA ise daha yüksek olarak bulunmuştur. Sünenindiyapoz sonrası bireylerin PE ve PC fraksiyonlarındaki 18:1n-9düzeyi, diyapoz öncesi bireylerden önemli derecede daha yüksekolarak tespit edilmiştir. Diyapoz sonrası erginlerin tüm PLaltsınıflarındaki doymamış yağ asitlerinin doymuş yağ asitlere oranı(UFA/SFA), diyapoz öncesi erginlerden daha yüksek bulunmuştur.

The Changes in Composition of Phospholipid Subclasses Fatty Acids Prepared from Prediapausing and Postdiapausing Adults of Sunn Pest, Eurygaster integriceps Put. (Heteroptera: Scutelleridae)

In this study, the changes in fatty acid composition of phospholipid (PL) subclasses such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS) of pre-diapausing and post-diapausing adults of sunn pest Eurygaster integriceps were investigated. Thin Layer Chromatography technique was used to separate phospholipid subclasses and Gas Chromatography equipment was used for the analysis of fatty acids. The dominant fatty acids observed in PL subclasses of E. integriceps were palmitic acid (16:0) among saturated fatty acids (SFAs), oleic acid (18:1n-9) among monounsaturated fatty acids (MUFAs) and linoleic acid (18:2n-6) among polyunsaturated fatty acids (PUFAs). It was found that the changes in fatty acid compositions from the PC, PE and PS fractions of prediapausing and postdiapausing adults were similar. In these fractions of postdiapausing adults, the percentages of 16: 0, stearic acid (18:0) and ∑SFA were lower than in prediapausing adults; however, palmitoleic acid (16: 1n-7, ∑MUFA, 18: 2n-6 and ∑PUFA were higher. The level of oleic acid in PE and PC fractions of postdiapausing individuals of the E. integriceps was determined to be significantly higher than prediapausing individuals. The ratio of unsaturated fatty acids to saturated fatty acids (UFA / SFA) in all PL subclasses of postdiapausing adults was found to be higher than those of prediapausing adults.

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