ATIK KAĞITTAN ELDE EDİLMİŞ SELÜLOZ NANOKRİSTALLERİ/GÜMÜŞ NANOPARTİKÜLLERİ NANOKOMPOZİT SENSÖRÜ İLE HİDROJEN PEROKSİTİN KOLORİMETRİK TESPİTİ

Bu çalışma; asit hidrolizi yöntemi ile atık kâğıtlardan selüloz nanokristallerinin (CNC) üretimi, üretilen CNC ve AgNO3 çözeltisinin karıştırılması ve ortama NaBH4 ilavesi ile Ag+ iyonlarının Ag0’a indirgenmesi sonucu CNC/AgNPs nanokompozit malzemenin sentezlenmesi, CNC/AgNPs malzemesinin UV-vis spektroskopisi, DLS, SEM, EDX ve XRD yöntemleri ile karakterizasyonu ve H2O2’nin kolorimetrik tespitinde sensör olarak kullanılabilirliği çalışmalarının sonuçlarını içermektedir. CNC/AgNPs nanokompoziti ile H2O2’nin nicel analizi için 0,001-1,0 mM H2O2 derişimi aralığında bir kalibrasyon doğrusu oluşturulmuş, CNC/AgNPs’nin H2O2’yi minimum tespit sınırı 0,7864 μM olarak belirlenmiştir. CNC/AgNPs’nin H2O2’ye seçiciliğinin belirlenmesi amacıyla çeşitli bileşenlerin varlığında CNC/AgNPs ile H2O2’nin kolorimetrik tespiti çalışmaları gerçekleştirilmiş; KCl, Al(NO3)3, oksalik asit, laktik asit, glikoz, laktoz, maltoz, fruktoz, sakkaroz, üre bileşenlerinin varlığında CNC/AgNPs’nin H2O2’nin kolorimetrik tespitinde etkin bir şekilde kullanılabileceği sonucuna varılmıştır.

Anahtar Kelimeler:

Atık kâğıt, selüloz nanokristalleri, gümüş nanopartikülleri, nanokompozit malzeme, H₂O₂ tespiti, kolorimetrik sensör

Colorimetric Detection of Hydrogen Peroxide with Cellulose Nanocrystals Obtained From Waste Paper/ Silver Nanoparticles Nanocomposite Sensor

This study includes the results of the studies of the production of cellulose nanocrystals (CNC) from waste paper by the acid hydrolysis method, the synthesis of nanocomposite material CNC/AgNPs as a result of mixing the produced CNC and AgNO3 solution and then reducing the Ag+ ions to Ag0 by adding NaBH4 to the medium, the characterization of CNC/AgNPs by UV-vis spectroscopy, DLS, SEM, EDX and XRD methods, and its usability as a sensor in the colorimetric detection of H2O2. For the quantitative analysis of H2O2 with CNC/AgNPs nanocomposite, a calibration line was created in the range of 0.001-1.0 mM H2O2 concentration, and the minimum detection limit for H2O2 of CNC/AgNPs was determined as 0.7864 μM. In order to determine the selectivity of CNC/AgNPs to H2O2, the studies of the colorimetric detection of H2O2 with CNC/AgNPs was carried out in the presence of various components; it was concluded that CNC/AgNPs can be used effectively in the colorimetric detection of H2O2 in the presence of KCl, Al(NO3)3, oxalic acid, lactic acid, glucose, lactose, maltose, fructose, sucrose, urea components.

Keywords:

Waste paper, cellulose nanocrystals, silver nanoparticles, nanocomposite material, H₂O₂ detection, colorimetric sensor,

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Amirjani, A., Bagheri, M., Heydari, M., Hesaraki, S., 2016, “Label-free surface plasmon resonance detection of hydrogen peroxide; A bio-inspired approach”, Sensors and Actuators B: Chemical, Vol. 227, pp. 373–382.
Chudasama B., Vala A.K., Andhariya N., Mehta R.V., Upadhyay R.V., 2010, “Highly bacterial resistant silver nanoparticles: synthesis and antibacterial activities”, Journal of Nanoparticle Research, Vol. 12, pp. 1677–1685.
Diehn, M., Cho, R. W., Lobo, N. A., Kalisky, T., Dorie, M. J., Kulp, A. N., Qian, D., Lam, J. S., Ailles, L. E., Wong, M., Joshua, B., Kaplan, M. J., Wapnir, I., Dirbas, F. M., Somlo, G., Garberoglio, C., Paz, B., Shen, J., Lau, S. K., 2009, “Association of Reactive Oxygen Species Levels and Radioresistance in Cancer Stem Cells”, Nature, Vol. 458, No. 7239, pp. 780–783.
Farrokhnia, M., Karimi, S., Momeni, S., Khalililaghab, S., 2017, “Colorimetric sensor assay for detection of hydrogen peroxide using green synthesis of silver chloride nanoparticles: Experimental and theoretical evidence”, Sensors and Actuators B: Chemical, Vol. 246, pp. 979–987.
Henglein A., Giersig M., 1999, “Formation of colloidal silver nanoparticles: capping action of citrate”, The Journal of Physical Chemistry B, Vol. 103, pp. 9533–9539.
Hu, Y., Tang, L., Lu, Q., Wang, S., Chen, X., Huang, B., 2014, “Preparation of cellulose nanocrystals and carboxylated cellulose nanocrystals from borer powder of bamboo”, Cellulose, Vol. 21, No. 3, pp. 1611-1618.
Johar, N., Ahmad, I., Dufresne, A., 2012, “Extraction, preparation and characterization of cellulose fibres and nanocrystals from rice husk”, Industrial Crops and Products, Vol. 37, No. 1, pp. 93-99.
Lee, S. Y., Mohan, D. J., Kang, I. A., Doh, G. H., Lee, S., Han, S. O., 2009, “Nanocellulose reinforced PVA composite films: effects of acid treatment and filler loading”, Fibers and Polymers, Vol. 10, No. 1, pp. 77-82.
Li, N., Huang, J., Wang, Q., Gu, Y., and Wang, P., 2018, “A Reaction Based One- and Two-Photon Fluorescent Probe for Selective Imaging H2O2 in Living Cells and Tissues”, Sensors & Actuators: B. Chemical, Vol. 254, pp. 411–416.
Liu Z.G., Wang Y.L., Zu Y.G., Fu Y.J., Li N., Guo N., Liu R.S., Zhang Y.M., 2014, “Synthesis of polyethylenimine (PEI) functionalized silver nanoparticles by a hydrothermal method and their antibacterial activity study”, Materials Science and Engineering: C, Vol. 42, pp. 31–37.
Nitinaivinij, K., Parnklang, T., Thammacharoen, C., Ekgasit, S., Wongravee, K., 2014, “Colorimetric determination of hydrogen peroxide by morphological decomposition of silver nanoprisms coupled with chromaticity analysis”, Analytical Methods, Vol. 6, No. 24, pp. 9816–9824.
Olgaç, N., 2019, Nanokristalin Selülozun (CNC) Kimyasal Modifikasyonu ve Nanokompozit Malzeme Uygulamaları, Yüksek Lisans Tezi, Gebze Teknik Üniversitesi, Fen Bilimleri Enstitüsü, Gebze.
Orue, A., Santamaria‐Echart, A., Eceiza, A., Peña‐Rodriguez, C., Arbelaiz, A., 2017, “Office waste paper as cellulose nanocrystal source”, Journal of Applied Polymer Science, Vol. 134, No. 3, pp. 45257-45268.
Özkahraman, B., Işıl, A. C. A. R., Gök, M. K., Güçlü, G., 2014, “Poli (N-Vinilkaprolaktam) Mikrojellerinin Sentez Şartlarının Optimizasyonu”, Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, Vol. 14, No. 1, pp. 13-21.
Rivero, P. J., Ibañez, E., Goicoechea, J., Urrutia, A., Matias, I. R., Arregui, F. J., 2017, “A self-referenced optical colorimetric sensor based on silver and gold nanoparticles for quantitative determination of hydrogen peroxide”, Sensors and Actuators B: Chemical, Vol. 251, pp. 624-631.
Santmartí, A., Lee, K. Y., 2018, “Crystallinity and thermal stability of nanocellulose”, Nanocellulose and sustainability, 1st Edition, Edited by Koon-Yang Lee, CRC Press, Boca Raton, U.S.A.
Sofla, M. R. K., Brown, R. J., Tsuzuki, T., Rainey, T. J., 2016, “A comparison of cellulose nanocrystals and cellulose nanofibres extracted from bagasse using acid and ball milling methods”, Advances in Natural Sciences: Nanoscience and Nanotechnology, Vol. 7, No. 3, pp. 1-9.
Temple, M. D., Perrone, G. G., and Dawes, I. W., 2005, “Complex Cellular Responses to Reactive Oxygen Species”, Trends in Cell Biology, Vol. 15, No. 6, pp. 319–326.
Teodoro, K. B., Migliorini, F. L., Christinelli, W. A., & Correa, D. S., 2019, “Detection of hydrogen peroxide (H2O2) using a colorimetric sensor based on cellulose nanowhiskers and silver nanoparticles”, Carbohydrate polymers, Vol. 212, pp. 235-241.
Theivasanthi, T., Christma, F. A., Toyin, A. J., Gopinath, S. C., Ravichandran, R., 2018, “Synthesis and characterization of cotton fiber-based nanocellulose”, International journal of biological macromolecules, Vol. 109, pp. 832-836.
Vasileva, P., Donkova, B., Karadjova, I., Dushkin, C., 2011, “Synthesis of starch-stabilized silver nanoparticles and their application as a surface plasmon resonance-based sensor of hydrogen peroxide”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 382, No. 1-3, pp. 203-210.
Xu, X., Liu, F., Jiang, L., Zhu, J. Y., Haagenson, D., Wiesenborn, D. P., 2013, “Cellulose nanocrystals vs. cellulose nanofibrils: a comparative study on their microstructures and effects as polymer reinforcing agents”, ACS applied materials & interfaces, Vol. 5, No. 8, pp. 2999-3009.
Yavuz, T., 2016, Development of A New Spectrophotometric Method For The Determination of Hydrogen Peroxide, Yüksek Lisans Tezi, Ege Üniversitesi, Fen Bilimleri Enstitüsü, İzmir.
Zhang, L., Li, L., 2016, “Colorimetric detection of hydrogen peroxide using silver nanoparticles with three different morphologies”, Analytical Methods, Vol. 8, No. 37, pp. 6691-6695.
Zhang, X., Sun, H., Tan, S., Gao, J., Fu, Y., Liu, Z., 2019, “Hydrothermal synthesis of Ag nanoparticles on the nanocellulose and their antibacterial study”, Inorganic Chemistry Communications, Vol. 100, pp. 44-50.