Yaşar ŞAHİN, Mehmet Eray ALÇIĞIR, Ali ŞENOL, Hüseyin ÖZDEN, Hüsamettin EKİCİ, Ebru YILDIRIM, Miyase ÇINAR

Sıçanlarda Gentamisin ile İndüklenmiş Oksidatif Stres Aracılı Nefrotoksisiteye Karşı Krill Yağının Koruyucu Etkisi

Bu çalışmada, gentamisin'in neden olduğu nefrotoksisiteye karşı kril yağının koruyucu etkisinin değerlendirilmesi amaçlandı. Çalışmada yedi gün boyunca oral yolla kontrol ve ikinci grubuna (GI) distile su verilirken, üçüncü (GII) ve dördüncü (GIII) gruplarına 500mg/kg krill yağı verildi. Ayrıca çalışma boyunca subkutan yolla kontrol ve GIII gruplarına izotonik tuzlu su uygulanırken, GI ve GII gruplarına 80 mg/kg gentamisin uygulandı. Plazma alanin aminotransferaz (ALT) ve gama glutamiltransferaz (GGT), total kolesterol, üre ve kreatinin düzeylerine, hem plazma hem de böbrek doku süpernatından ise malondialdehit (MDA) ve total antioksidan kapasitesi (TAS) düzeylerine değerlendirildi. Histopatolojik olarak tubul ve glomeruluslardaki değişimler ile damarsal değişiklikler skorlanarak değerlendirildi. Üre düzeyi ve ALT aktivitesi GI gruba göre GII ve GIII verilen grupta anlamlı düzeyde düşük bulundu (p<0.001; p≤0.001). Sonuç olarak, oksidatif stres aracılı olarak histolojik düzeyde tubulde dejeneratif hasar ve glomerular değişikliklerin özellikle ALT, üre ve MDA düzeyleri artışıyla uyumlu olduğu görüldü. Bu bakımdan, krill yağı nefroprotektif bir gıda takviyesi olarak toksisite durumlarında tedaviye katkı sağlamak için kullanılabileceği önerilmektedir.

Anahtar Kelimeler:

Böbrek, Gentamisin, Kril yağı, Nefrotoksisite, Oksidatif stres

Protective Effect of Krill Oil Against Gentamicin Induced Oxidative Stress Mediated Nephrotoxicity in Rats

This study aimed to evaluate the protective effect of krill oil against nephrotoxicity caused by gentamicin. Distilled water was given orally to the control and second groups (GI) for seven days while 500 mg/kg krill oil was given to the third (GII), fourth (GIII) groups. In addition, isotonic saline was administered subcutaneously to the control and GIII groups throughout the study, while 80 mg/kg gentamicin was administered to the GI, and GII groups. Alanine aminotransferase (ALT) and gamma glutamyltransferase (GGT) peptidase, total cholesterol, urea, and creatinine levels in plasma and, malondialdehyde (MDA) and total antioxidant status (TAS) levels in both plasma and kidney tissue supernatant were evaluated. Histopathological changes in tubules and glomeruli and vascular changes were evaluated by scoring. Urea level and ALT activity were found to be significantly lower in the GII and GIII groups compared to the GI group (p<0.001; p≤0.001). As a result, it was observed that degenerative damage and glomerular changes in the tubule at the histological level mediated by oxidative stress were consistent with the increase in ALT, urea, and MDA levels. In this respect, it is suggested that krill oil can be used as a nephroprotective food supplement to contribute to treatment in cases of toxicity.

Keywords:

Gentamicin, Kidney, Krill oil, Nephrotoxicity, Oxidative stress,

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