Malaklı Köpeği Spermasının Dondurulabilirliği Üzerine Karnosik Asitin Etkisi

Sunulan çalışmanın amacı, dondurulmuş çözdürülmüş Malaklı Çoban Köpeği spermasında karnosik asitin motilite, morfoloji, canlılık ve membran bütünlüğü parametrelerine etkisini belirlemektir. Bu çalışmada beş baş Malaklı köpeğine ait ejakülatların spermatozoon bakımından zengin kısmı kullanıldı. Ejakülatlar birleştirildi ve beş eşit gruba bölündü. Kontrol grubu temel Tris sulandırıcısıyla sulandırıldı ve diğer gruplar 10 µM, 50 µM, 100 µM, 200 µM karnosik asit içeren gruplar oluşturuldu. Bir buçuk saat ekilibrasyonun ardından, payetler azot buharında donduruldu ve daha sonra sıvı azot içinde depolandı. Ardından dondurulmuş payetler 37oC sıcak suda çözdürüldü ve motilite, morfoloji, canlılık ve membran bütünlüğü parametreleri değerlendirildi. En yüksek motilite ve HOST-Eozin değerleri 10 µM’lık grupta elde edildi ve sırasıyla %65,00±2,23 ve %69,83±0,70 olarak belirlendi, en düşük anormal spermatozoon oranı ise 9,50±1,52 ile yine10 µM’lık gruptan elde edildi ve bu gruplardaki fark (p<0.05) kontrol grubuna göre istatistiki olarak önemli bulundu. Sonuç olarak Malaklı köpeği spermasının dondurulmasında 10 µM karnosik asitin, motilite, spermatozoon morfolojisi, membran bütünlüğü ve canlılığı açısından kontrol grubuna kıyasla koruyucu bir etki gösterdiği belirlenmiştir.

Anahtar Kelimeler:

Karnosik asit, kriyoprezervasyon, Malaklı köpeği, sperma

The Effect of Carnosic Acid on Semen Freezability in Malaklı Shepherd Dogs

The objective of the study was to determine the effect of carnosic acid, to Malakli Shepherd dog semen motility, morphology, viability and membran integrity parameters after frozen-thawed prosess. Five animal were used in this study and the sperm rich portion of the ejaculates were mixed and divided in to five equal groups. Each group extended with either tris as a control group and containing carnosic acid 10 µM, µM, 100 µM, 200 µM. Following equilibration for one and a half hour, the straws were frozen in nitrogen vapor and then stored in liquid nitrogen. Later, the frozen straws were thawed in a water bath for motility, morphology, viability and membran integrity parameters examination. The highest motilities and HOST-Eosin test results were detected in 10 µM group at 65.00±2.23 % and 69.83±0.70 % respectively, the lowest abnormal sperm rate were detected in 10 µM group at 9.50±1.52 % and the differences in these groups (p <0.05) were statistically significant compared to the control group. With regard conclusion, it was determined that the doses of 10 µM of carnosic acid added to extender in freezing of Malakli dog sperm showed a protective effect compared to the control group in terms of motility, spermatozoon morphology, membrane integrity and viability.

Keywords:

Carnosic acid, cryopreservation, Malaklı shepherd dogs, sperm,

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