The pathogenesis of frostbite, a kind of ischemia-reperfusion injury, is not clear in spite of various researches, The present study was aimed to assess the determination of nitric oxide in frostbite injury. Frozeh rabbit ears were used in this study. Serum nitric oxide levels were measured. Eight New Zealand albino rabbits were subjected to the study. The right ears of rabbits were immersed into ethyl alcöhol 100 % at - 20° Celsius and the left ears were not subjected to the cold injury. Blood samples were taken fröm vein of the frozen ears at re-warming period and at the 4th hour. The control blood samples were taken from the left ears. Decreased nitric oxide levels were determined at the 4th hour in blood samples of the frozen ears (p<0.05). These data suggest that nitric oxide may have an important role in frostbite.