Sulu Ortamlardaki Formaldehitin Ölçümü İçin Biyosensör Dizaynı

Bu çalışmada, sularda ve havada izlenmesi gereken önemli kirletici parametrelerinden olan formaldehitin güvenli, seçici, hassas ve hızlı ölçülmesi amacıyla polipirol film ile hazırlanmış çalışma elektrodundan oluşan formaldehit biyosensörü geliştirilmiştir. Formaldehit dehidrogenaz enzimi polipirol film boşluklarına adsorbe edilerek elektroda bağlanmıştır. Sistemin sinyal gücünü artırmak amacıyla elektron aracısı olarak naftokinon kullanılmıştır. Dizayn edilen biyosensörden yüksek performansta sinyal alınabilmesi için önemli parametreler optimize edilmiştir. Biyosensör analitik parametreleri hesaplanarak elde edilen sonuçlar tartışılmıştır. Biyosensör ile, sulu ortamlarda ppb seviyesindeki formaldehit konsantrasyonları 12 saniyede ve yüksek hassasiyetle ölçülebilmiştir

Anahtar Kelimeler:

Biyosensör, Formaldehit, Formaldehit dehidrogenaz

Biosensor Design for Formaldehyde Measurement in Aqueous Mediums

In this study, a formaldehyde biosensor comprised of working electrode prepared with Polypyrrole film has been developed in order to get safe, selective, sensitive and fast detection of formaldehyde which should be detected in water and air as an important pollutant parameter. The enzyme formaldehyde deyhdrogenase has been immobilized onto the electrode by the adsorption into the pores of the Polypyrrole film. Napthoquinone has been used as an electron mediator to improve the power signal of the system. The important parameters have been optimized to get high-performanced signals from the designed biosensor. Results obtained from the calculation of the analytical parameters have been discussed. Formaldehyde concentrations at ppb level in aqueous mediums could be measured with high sensitivity at 12 seconds by the biosensor.

Keywords:

Biosensor, Formaldehyde, Formaldehyde dehydrogenase,

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