Pre-emptive Use of Riboflavin in a Rat Model of Bilateral Cavernous Nerve Injury

Objective: Erectile dysfunction is commonly encountered after radical prostatectomy due to cavernous nerve injury (CNI). We investigated the effects of riboflavin (Rb) on bilateral CNI in a rat model. Materials and Methods: Twenty-four male rats were divided into four groups: control (C), patients with bilateral CNI, those with CNI receiving postoperative Rb treatment (CNI+Rb), and those with CNI receiving pre- and post-operative Rb treatment (Rb+CNI+Rb). Bilateral CNI was performed in all groups except for C. The CNI+Rb group was treated with 30 mg/kg Rb daily after CNI for two weeks; the Rb+CNI+Rb group was treated with 30 mg/kg Rb daily one week before CNI and then for two weeks after injury. Mean arterial pressure (MAP) and intracavernosal pressure (ICP) were measured 14 days after CNI in all groups. Tissue malondialdehyde, cyclic guanosine monophosphate, nerve growth factor, superoxide dismutase and total nitric oxide synthase (NOS) activities, neuronal NOS (nNOS) and inducible NOS (iNOS) were analyzed. Results: ICP/MAP ratio was significantly lower in the CNI (p

Bilateral Kavernozal Sinir Hasarı Sıçan Modelinde Preemptif Riboflavin Tedavisinin Etkileri

Amaç: Çalışmamızın amacı radikal prostatektomi modelinde erektil disfonksiyon tedavisinde riboflavinin (Rb) olası etkilerinin araştırılmasıdır. Gereç ve Yöntem: Yirmi dört erkek sıçan kontrol (K), bilateral kavernoz sinir hasarı (KSH), KSH+Rb ve Rb +KSH+ Rb olmak üzere 4 gruba ayrıldı. Ön-tedavili KSH grubunda cerrahiden 1 hafta önce başlayarak toplam 3 hafta ve KSH+Rb grubuna ise cerrahiden sonra 2 hafta süreyle 30 mg/kg dozunda Rb uygulandı. Deney sonunda intrakavernosal basınç (İKB) ve ortalama arteryel basınç (OAB) ölçümü yapıldı. Kavernoz doku örneklerinde malondialdehit (MDA), siklik guanozin monofosfat (c-GMP) ve sinir büyüme faktörü (NGF) düzeyleri ile süperoksid dismutaz (SOD) ve total nitrik oksit sentaz (NOS) aktiviteleri ölçüldü. Dokuların indüklenebilir NOS ve nöronal NOS protein ekspresyonları Western blot yöntemi ile tayin edildi. Bulgular: KSH, İKB/OAB değerlerinde anlamlı azalmaya (p

Kaynakça

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