The Cytotoxicity and the Antimicrobial Activities of Arum italicum Miller
The Cytotoxicity and the Antimicrobial Activities of Arum italicum Miller
In this study, the cytotoxicity potential and antibacterial and antifungal activities of two different extracts of fresh leaves of Arum italicum Miller were investigated. The saponin extract and the alkaloid extract were investigated by Brine Shrimp (Artemia salina L.) lethality bioassay method for evaluation of cytotoxic effect. Umbelliferone was used as standard, and both of the extracts showed cytotoxic effect by the Brine Shrimp (Artemia salina L.) lethality bioassay method. According to the LC50 results, saponin extract was found to be the most active extract, and classifiable as moderate to highly toxic (LC50 237.1437 mg/mL-1). Although, the alkaloid extract was showed cytotoxic activity lower than Umbelliferone, LC50 750.1920 mg/mL-1 value was classifiable as mildly cytotoxic. Antibacterial and antifungal activities were tested by the agar well diffusion and tube dilution methods. 6 bacteria and 6 yeasts were used in the methods Meropenem and Fluconazole were the standards of the methods. The saponin extract was found to be effective against Staphylococcus epidermidis and Sthaphylococcus aureus compared with Meropenem. Both of the extracts were not found to be effective against fluconazole as antifungal activity point of view. According to the all studied activity results the saponin contents of the fresh leaves of the plant emphasizing the antimicrobial activity. And, both of the extracts have cytotoxic activity potential.
___
- [1] Davis PH, Flora of Turkey and the East Aegean Islands, Edinburgh University Pres, England, 1965.
- [2] Hacıoğlu M, Kulaksız B, Alpınar K, Hacıosmanoğlu E, Tan SB. In vitro antimicrobial, antibiofilm and cytotoxic activities of the extracts of Arum italicum Miller leaves. Farmacia. 2021; 69(5): 849-854. [CrossRef]
- [3] Baytop T, Theraphy with Medicinal Plants in Turkey (past and present) İstanbul University Publication, Türkiye, 1984.
- [4] PDR for herbal Medicines. Medicinal Economics Company, New Jersey, 2000.
- [5] Azab A. Arum: A plant genus with great medicinal potential. Eur Chem Bull. 2017; 6(2): 59-68. [CrossRef]
- [6] Ağalar HZ, Çiftçi GA, Göger F, Kırımer N. Activity guided fractionation of Arum italicum Miller tubers and the LC/MS-MS profiles. Rec Nat Prod. 2018; 12(1): 64-75. [CrossRef]
- [7] Gibernau M, Macquart D, Przetak G. Pollination in the Genus Arum – a review. Aroideana. 2004; 27: 148-166.
- [8] Nguta JM, Mbaria JM, Gathumbi PK, Kabasa JD, Kiama SG. Biological screening of Kenya medicinal plants using artemia salina (ARTEMIIDAE). Pharmacologyonline. 2011; 2: 458-478. [CrossRef]
- [9] Dey PM, Harborner JB, Methods in Plant Biochemistry, San Diego: Academic Press, New York,USA, 1991. [10] Kolak U, Ulusoylu M. Constituents of Delphinium uncinatum. Scientia Pharmaceutica. 1998; 66(4): 381-385. [CrossRef]
- [11] Çetin ET, General and Practic Microbiology, Sermet Press, Istanbul, 1973.
- [12] National Comittee for clinical Laboratory Standards, Reference Method for Broth Dilution Susuceptibility Testing of Yeast: Propsed Standard M27-P, National Committee for Clinical Laboratory Standards, Villanova, PA, 1992
- [13] National Comittee for clinical Laboratory Standards, Reference Method for Dilution Antimicrobial Susuceptibility Tests for Bacteria That Grow Aerobically, Approved Standard M7-A3, National Committee for Clinical Laboratory Standards, Villanova, PA, 1993.
- [14] Finney DJ, Probit analysis, Cambridge University Press, New York, 1971.