Khalid HUSSAIN, Naureen SHEHZADI, Ayisha SHAUKAT, Nadeem Irfan BUKHARI

Simultaneous determination of paracetamol and lidocaine hydrochloride in detamol injection using RP-HPLC

The current study describes the simultaneous determination of paracetamol and lidocaine hydrochloride in Detamol injection using RP-HPLC with fluorescent light detection (FLD). Samples elution was done through the HPLC column (C18-XDB, 5μm, 4.6 × 150 mm) with isocratic mobile phase- a mixture of water, acetonitrile and tetrahydrofuran (90:5:5, v/v/v) at a flow rate of 1.0 ml/min using FLD detector (250 nm excitation and 410 nm emission). The analytical method was linear for both the analytes, over the investigated range with the correlation coefficient (R2) = 0.993 - 0.995. The limit of detection (LOD) and limit of quantification (LOQ) were as: paracetamol 0.894 μg/ml, 2.98 μg/ml, respectively, and lidocaine HCl 0.53μg/ml, 1.78μg/ml, respectively. The analytical method was reliable, precise and accurate (RSD < 5%). The developed method was successfully applied to quantify paracetamol and lidocaine HCl in Detamol injection. The results of current study indicated that developed method is linear, precise, accurate and sensitive, hence may be used for quality control purposes.

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[1] Duggan ST, Scott LJ. Intravenous paracetamol (acetaminophen). Drugs. 2009; 69(1):101-13. [CrossRef]
[2] Visapää H, Taari K. Combination of paracetamol, codeine and lidocaine for pain relief during transrectal ultrasound guided biopsy of the prostate. Scand J Surg. 2009; 98(1):55-57. [CrossRef]
[3] Hafsa D, Chanda S, J Prabhu P. Simultaneous HPLC determination of methocarbamol, paracetamol and diclofenac sodium. J Chem. 2011; 8(4):1620-1625. [CrossRef]
[4] Hewala II. Stability-Indicating Hplc Assay for Paracetamol, Guaiphenesin, Sodium Benzoate and Oxomemazine in Cough Syrup. Anal Lett. 1994; 27(1):71-93. [CrossRef]
[5] Altun ML. HPLC method for the analysis of paracetamol, caffeine and dipyrone. Turk J Chem. 2002; 26(4):521-528.
[6] Karunakaran K, Navaneethan G, Elango KP. Development and validation of a stability-indicating RP-HPLC method for simultaneous determination of paracetamol, tramadol HCl and domperidone in a combined dosage form. Trop J Pharm Res. 2012; 11(1):99-106. [CrossRef]
[7] Hazai E, Simon-Trompler E, Czira G, Vereczkey L, Monostory K. New LC method using radioactivity detection for analysis of toxic metabolite of acetaminophen (Paracetamol). Chromatographia. 2002; 56(1):75-78. [CrossRef]
[8] Torres Pio dos Santos W, Nascimento de Almeida EG, Alves Ferreira HE, Gimenes DT, Richter EM. Simultaneous flow injection analysis of paracetamol and ascorbic acid with multiple pulse amperometric detection. Electroanalysis: An International Journal Devoted to Fundamental and Practical Aspects of Electroanalysis. 2008; 20(17):1878-1883. [CrossRef]
[9] Brown JK, Shapazian L, Griffin GD. A rapid screening procedure for some “street drugs” by thin-layer chromatography. J Chromatogr A. 1972; 64(1):129-33. [CrossRef]
[10] Zivanovic L, Agatonovic-Kustrin S, Vasiljevic M, Nemcova I. Comparision of high-performance and thin-layer chromatographic methods for the assay of lidocaine. J Pharm Biomed. 1996; 14(8-10):1229-1232.[CrossRef]
[11] Devarajan PV, Adani MH, Gandhi AS. Simultaneous determination of lidocaine hydrochloride and phenylephrine hydrochloride by HPTLC. J Pharm Biomed. 2000; 22(4):685-690.[CrossRef]
[12] International Conference on Harmonization guidelines ICH Q2 (R1) (2005). Validation of analytical procedures: Text and methodology. International Conference on Harmonization, International Federation of Pharmaceutical Manufacturers and Associations, Geneva. Retrieved fromhttp://www.ich.org/fileadmin/Public_Web_ Site/ICH_Products/Guidelines/Quality/Q2_R1/S tep4/Q2_R1__Guideline.pdf. [CrossRef]
[13] Vaidya VV, Singh GR, Choukekar MP, Kekare MB. Simultaneous RP HPLC determination of aceclofenac, paracetamol and tizanidine in pharmaceutical preparations. J Chem. 2010; 7(1):260-264. [CrossRef]
[14] Abraham I, Fawcett JP, Kennedy J, Kumar A, Ledger R. Simultaneous analysis of lidocaine and bupivacaine enantiomers in plasma by high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl. 1997;703(1- 2):203-208. [CrossRef]
[15] Kang L, Jun HW, McCall JW. HPLC assay of Lidocaine in plasma with solid phase extraction and UV detection. J Pharm Biomed. 1999; 19(5):737-745. [CrossRef]
[16] Plenis A, Konieczna L, Miękus N, Bączek T. Development of the HPLC method for simultaneous determination of lidocaine hydrochloride and tribenoside along with their impurities supported by the QSRR approach. Chromatographia. 2013; 76(5-6):255-265. [CrossRef]
[17] Belal T, Awad T, Clark R. Determination of paracetamol and tramadol hydrochloride in pharmaceutical mixture using HPLC and GC-MS. J Chromatogr Sci. 2009; 47(10): 849-854. [CrossRef]