Leonny Yulita HARTIADI, Agnes Anania Triavika SAHAMASTUT

Protective effect of Caesalpinia sappan L. extract against H2O2-induced oxidative stress on HaCaT and its formulation as antioxidant cream

Oxidative stress arises from excessive generation of reactive oxygen species and it is known to be themajor factor that accelerates skin aging and involved in the development of skin inflammatory disorders. Therefore,there is a need to develop cosmetics products containing active ingredient with potent antioxidant effect. One ofmedicinal plants that is potential to be utilized as an active ingredient for cosmetics is Caesalpinia sappan L. Previousstudies demonstrated the potent antioxidant activity of C. sappan in numerous in vitro scavenging free radicals assay. The present study aims to evaluate the antioxidant of C. sappan in a biological system and further formulates the extract in cream formulation. Powdered C. sappan was macerated with 96% ethanol and the dried extract was assessed for the cytotoxic and antioxidant effect on human keratinocytes cell line using MTS assay. Several cream formulations containing non-toxic concentration of C. sappan extract were made and evaluated. Cytotoxicity assay showed that C. sappan extract had an IC50 of 146.8±2.6 µg/ml. At a concentration of 15.625 µg/ml, the extract exhibited a protective effect against oxidative stress induced by H2O2 that was comparable to vitamin E. All of the formulated creams were o/w cream type, homogenous and had white-pinkish color. pH were in a range of 5.07±0.01 – 6.51±0.04 with shearthinning properties. Following freeze-thaw cycle, the formulas were found to be stable and no significant changes were observed, except for viscosity. This study demonstrates that several cream formulations of C. sappan extract have been successfully formulated with proven antioxidant property.

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[1] Yoon Y, Lee YM, Song S, Lee YY, Yeum KJ. Black soybeans protect human keratinocytes from oxidative stress‐ induced cell death. Food Sci Nutr. 2018; 6(8): 2423-2430. [CrossRef]
[2] Gragnani A, Cornick SM, Chominski V, de Noronha SM, de Noronha SA, Ferreira LM. Review of Major Theories of Skin Aging. Adv Aging Res. 2014; 3: 265-284. [CrossRef]
[3] Lan CC, Hung YT, Fang AH, Ching-Shuang W. Effects of irradiance on UVA-induced skin aging. J Dermatol Sci. 2019; 94(1): 220-228. [CrossRef]
[4] Woodby B, Penta K, Pecorelli A, Lila MA, Valacchi G. Skin Health from the Inside Out. Annu Rev Food Sci Technol. 2020; 11: 235-254. [CrossRef]
[5] Saenjum C, Chaiyasut C, Kadchumsang S, Chansakaow S, Suttajit M. Antioxidant activity and protective effects on DNA damage of Caesalpinia sappan L. extract. J Med Plant Res. 2010; 4(15): 1594-1608. [CrossRef]
[6] Febriyenti F, Suharti N, Lucida H, Husni E, Sedona O. Karakterisasi dan Studi Aktivitas Antioksidan dari Ekstrak Etanol Secang (Caesalpinia sappan L.). J Sains Farm Klin. 2018; 5(1): 23-27. [CrossRef]
[7] Mitani K, Takano F, Kawabata T, Allam AE, Ota M, Takahashi T, Yahagi N, Sakurada C, Fushiya S, Ohta T. Suppression of melanin synthesis by the phenolic constituents of sappanwood (Caesalpinia sappan). Planta Med. 2013; 79(01): 37-44. [CrossRef]
[8] Setiawan F, Yunita O, Kurniawan A. Uji Aktivitas Antioksidan Ekstrak Etanol Kayu Secang (Caesalpinia sappan) Menggunakan Metode DPPH, ABTS, dan FRAP. MPI. 2018; 2(2): 82-99. [CrossRef]
[9] Nirmal NP, Rajput MS, Prasad RG, Ahmad M. Brazilin from Caesalpinia sappan heartwood and its pharmacological activities: A review. Asian Pac J Trop Med. 2015; 8(6): 421-30. [CrossRef]
[10] Domínguez-Perles R, Guedes A, Queiroz M, Silva AM, Barros AI. Oxidative stress prevention and anti-apoptosis activity of grape (Vitis vinifera L.) stems in human keratinocytes. Food Res Int. 2016; 87: 92-102. [CrossRef]
[11] Ramanauskienė K, Stelmakiene A, Majienė D. Assessment of lemon balm (Melissa officinalis L.) hydrogels: quality and bioactivity in skin cells. Evid Based Complement Alternat Med. 2015; 2015: 1-7. [CrossRef]
[12] Srisawat T, Chumkaew P, Heed-Chim W, Sukpondma Y, Kanokwiroon K. Phytochemical screening and cytotoxicity of crude extracts of Vatica diospyroides symington type LS. Trop J Pharm Res. 2013; 12(1): 71-76. [CrossRef
[13] Jenie RI, Handayani S, Susidarti RA, Udin Z, Meiyanto E. Cytotoxic and Antimetastasis Effect of Ethyl Acetate Fraction from Caesalpinia sappan L. on MCF-7/HER2 Cells. IJCC. 2017; 8(1): 42-50. [CrossRef]
[14] Bukke AN, Hadi FN, Babu KS. In vitro studies data on anticancer activity of Caesalpinia sappan L. heartwood and leaf extracts on MCF7 and A549 cell lines. Data Brief. 2018; 19: 868-877. [CrossRef]
[15] Hung TM, Hai NX, Nhan NT, Quang TT, QUAN TL, Cuong TD, Dang NH, Dat NT. Cytotoxic activity of new phenolic compounds from Vietnamese Caesalpinia sappan. Biosci Biotechnol Biochem. 2013; 77(12): 2378-82. [CrossRef]
[16] Nirmal NP, Panichayupakaranant P. Antioxidant, antibacterial, and anti-inflammatory activities of standardized brazilin-rich Caesalpinia sappan extract. Pharm Biol. 2015; 53(9): 1339-1343. [CrossRef]
[17] Batubara I, Mitsunaga T, Ohashi H. Brazilin from Caesalpinia sappan wood as an antiacne agent. J Wood Sci. 2010; 56(1): 77-81. [CrossRef]
[18] Chen M X, Alexander K S, Baki G. Formulation and evaluation of antibacterial creams and gels containing metal ions for topical application. J Pharm (Cairo). 2016; 2016: 1-10. [CrossRef]
[19] Walters K., Jones WM. Measurement of Viscosity. In: Boyes W. Instrumentation Reference Book 4th edition. Butterworth-Heinemann, Oxford, 2010, pp.69-75.
[20] Simões A, Veiga F, Vitorino C. Developing cream formulations: renewed interest in an old problem. J Pharm Sci. 2019; 108(10): 3240-3251. [CrossRef]
[21] Banerjee K., Thiagarajan N, Thiagarajan P. Formulation and characterization of a Helianthus annuus‐alkyl polyglucoside emulsion cream for topical applications. J Cosmet Dermatol. 2019; 18(2): 628-637. [CrossRef]
[22] VH ES, Utomo SB, Syukri Y, Redjeki T. Phytochemical screening and analysis polyphenolic antioxidant activity of methanolic extract of white dragon fruit (Hylocereus undatus). Indones J Pharm. 2012; 23(1): 60-64. [CrossRef]