Anil Kumar SINGH, Shanti Bhushan MISHRA, Varsha CHAUDHARY, Shradhanjali SINGH

Euryale ferox Salisb ameliorates arthritis in experimental animals

The plant Euryale ferox salisb family (Nymphaeaceae) commonly known as prickly water lily have been traditionally used in the treatment of rheumatism hence in the present study, investigation of anti-arthritic activity was accomplished using 50% v/v unproccessed ethanolic extract of leaves & chloroform fraction in arthritic models. The 50% ethanolic extract (EFEE) was separated into four fractions including n-hexane (EFHF), chloroform (EFCF), ethyl acetate (EFEA) and methanol (EFMF). Preliminary phytochemical screening and TLC were carried out on extract and all four fractions. The induction was accomplished by intraplantar injection of formaldehyde (2%v/v), turpentine (10%v/v) and complete Freund’s adjuvant (CFA) in left hind paw of wistar albino rats. Evaluation of anti-arthritic potential was been done by considering paw volume, body weight, joint diameter, arthritic score, serum enzymes, blood parameters and histopathological examination of rats blood and tissues. Extract and fraction were tested by phytochemical screening which signifies the existence of steroids, flavonoids, amino acid, alkaloid and phenolic compounds. In acute and chronic arthritic models, the plant had a dose-dependent anti-arthritic effect. The chloroform fraction (200mg/kg b.w) had a more significant effect than the 50% ethanolic extract. Hence, Euryale ferox ameliorates hematological indices, body weight, and histopathological alterations in a significant way.

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[1] Jeffrey AS. Rheumatoid arthritis. Ann Intern Med. 2019; 170(1): ITC1-ITC16. [CrossRef]
[2] Tripathi KD, Essentials of Medical Pharmacology, eighth ed., Jaypee Brothers Medical Publishers, New Delhi 2019.
[3] Tabish SA. Complementary and alternative healthcare: Is it evidence-based?. Int J Health Sci (Qassim). 2008; 2(1): V-IX.
[4] Nehal N, Mann S, Gupta RK. Two promising underutilized grains a review. Indian J Tradit Knowl. 2015; 14(3): 416-422.
[5] Mittal R, Sharma S, Mittal A. A critical review on ethnobotanical and pharmacological aspects of Euryale ferox Salisb. Pharmacog J. 2020; 12(6): 1444-1454. [CrossRef]
[6] Ahmed D, Sharma M, Kumar V, Bajaj HK, Verma A. 2β-hydroxybetulinic acid 3β-caprylate: an active principle from Euryale ferox Salisb. seeds with antidiabetic, antioxidant, pancreas & hepatoprotective potential in streptozotocin induced diabetic rats. J Food Sci Technol. 2015; 52(9): 5427-5441.[CrossRef]
[7] Baek SH, Nam IJ, Kwak HS, Kim KC, Lee SH. Cellular anti-melanogenic effects of a Euryale ferox seed extract ethyl acetate fraction via the lysosomal degradation machinery. Int J Mol Sci. 2015; 16(5): 9217-9235. [CrossRef]
[8] Das S, Der P, Raychaudhuri U, Maulik N, Das DK. The effect of Euryale ferox (Makhana), an herb of aquatic origin, on myocardial ischemic reperfusion injury. Mol Cell Biochem. 2006; 289(1-2): 55-63. [CrossRef]
[9] Negi JS, Jugran V, Kasliwal N. Development of non-effervescent floating matrix tablets based on Euryale ferox seeds. Asian J Pharm. 2011; 5: 93-100.
[10] Jana BR, Idris M. Anti-aging amino acids in Euryale ferox (Salisb.): a review. Adv Plants Agric Res. 2018; 8(1): 39‒43. [CrossRef]
[11] He S, Wang D, Zhang Y, Yang S, Li X, Wei D, Zhang M, Qin J. Chemical components and biological activities of the essential oil from traditional medicinal food, Euryale ferox Salisb seeds. J Essent Oil-Bear Plants. 2019; 22(1) :73-81. [CrossRef]
[12] Row LC, Ho JC, Chen CM. Cerebrosides and tocopherol trimers from the seeds of Euryale ferox. J Nat Prod. 2007; 70(7): 1214-1217. [CrossRef]
[13] Song CW , Wang SM, Zhou LL, Hou FF, WangK J, Han QB, Li N, Cheng YX. Isolation and identification of compounds responsible for antioxidant capacity of Euryale ferox seeds. J Agric Food Chem. 2011; 59(4): 1199- 1204. [CrossRef]
[14] Lee SE, Ju EM, Kim JH. Antioxidant activity of extracts from Euryale ferox seed. Exp Mol Med 2002; 34(2): 100-106. [CrossRef]
[15] Kirtikar KR, Basu BD, Indian medicinal plants, second ed., International book distributors, Dehradun, Uttaranchal 2005.
[16] Fischer BD, Adeyemo A, O-Leary ME, Bottaro A. Animal models of rheumatoid pain: experimental systems and insights. Arthritis Res Ther. 2017; 19(1): 146. [CrossRef]
[17] Opriessnig T, Gimenez-Lirola LG, Halbur PG. Polymicrobial respiratory disease in pigs. Anim Health Res Rev. 2011; 12(2): 133-148.[CrossRef]
[18] Murakami M, Hirano T. The molecular mechanisms of chronic inflammation development. Front Immunol. 2012; 3: 323. [CrossRef]
[19] Libregts SF, Gutiérrez L, de Bruin AM, Wensveen FM, Papadopoulos P, van Ijcken W, Ozgur Z, Philipsen S, Nolte MA. Chronic IFN-γ production in mice induces anemia by reducing erythrocyte life span and inhibiting erythropoiesis through an IRF-1/PU.1 axis. Blood. 2011; 118(9): 2578–2588. [CrossRef]
[20] Sadik CD, Kim ND, Luster AD. Neutrophils cascading their way to inflammation. Trends Immunol. 2011; 32(10): 452-460. [CrossRef]
[21] Ansar W, Ghosh S. Biology of C Reactive Protein in Health and Disease. In: Ansar W, Ghosh S. (Eds). Inflammation and Inflammatory Diseases, Markers, and Mediators: Role of CRP in Some Inflammatory Diseases. Springer India Publisher, New Delhi, 2016, pp. 67-101.
[22] Shadick NA, Cook NR, Karlson EW, Ridker P M, Maher N E, Manson J E, Buring JE, Lee IM. C-reactive protein in the prediction of rheumatoid arthritis in women. Arch Intern Med. 2006; 166(22): 2490- 2494.[CrossRef]
[23] Li Y, Kakkar R, Wang J. In vivo and in vitro approach to anti-arthritic and anti-inflammatory effect of crocetin by alteration of nuclear factor-E2-related factor 2/hem oxygenase (HO)-1 and NF-κB expression. Front Pharmacol. 2018; 9: 1341. [CrossRef]
[24] Yap HY, Tee SZ, Wong MM, Chow SK, Peh SC, Teow SY. Pathogenic Role of Immune Cells in Rheumatoid Arthritis: Implications in Clinical Treatment and Biomarker Development. Cells. 2018; 7(10): 161. [CrossRef]
[25] Rehman Q, Lane NE. Bone loss: Therapeutic approaches for preventing bone loss in inflammatory arthritis. Arthritis Res. 2001; 3(4): 221–227. [CrossRef]
[26] Harborne JB, Method of extraction and isolation: Phytochemical Methods, third ed., Chapman & Hall, London, UK 1998.
[27] OECD, Organisation for Economic Co-operation and Development. https://ntp.niehs.nih.Gov/ iccvam/suppdocs/feddocs/oecd/oecd_gl423.pdf (accessed on february 8, 2022)
[28] Singh S, Mishra SB, Mukerjee A. Clerodendrum serratum (L.) Moon leaf extract and its chloroform fraction attenuates acute and chronic arthritis in albino rats. Biocatal Agric Biotechnol. 2019; 22: 101399.[CrossRef]
[29] Kumar VL, Roy S, Sehgal R, Padhy BM. A comparative study on the efficacy of rofecoxib in monoarticular arthritis induced by latex of Calotropis procera and Freund’s complete adjuvant. Inflammopharmacol. 2006; 14: 17–21. [CrossRef]