Previously, some different techniques had been presented in terms of precise dissection and separation of tumors from surrounding brain. Purpose of this study was to describe the effect of vibration in the surgical dissection and separation of malignant brain tumors. We recently developed an instrument for micro-dissection and separation of brain lesions from brain tissue. This instrument was produced in a medical instrument producing factory in Samsun. This instrument has the capability of producing mechanical vibration. The holding and using of this instrument was described. In this study, we used this instrument in four high grade glial tumors for dissection and separation of these lesions from surrounding brain tissue. Dissection and separation were graded as poor, moderate and good. Vibrato-dissection technique was superior to those of conventional technique in the aspect of dissection and separation of brain tissue. The capability of dissection and separation with this instrument during surgical treatment of brain tumors may enhance dissection and separation of lesions. Key Words: Micro-vibration, glial tumors, dissection, separation.