Mikail KARA, Cennet RAĞBETLİ, Necat KOYUN, Neşe ÇÖLÇİMEN, Murat RAĞBETLİ

The stereological ratio of stromal/parenchymal components in postnatal prostate tissue of rats prenatally treated with diclofenac sodium

Since diclofenac sodium extends the pregnancy and affects the fetus, our study aimed to stereologically investigate the components of the rat prostate with prenatally treated with diclofenac sodium. The rats were left overnight to mate. Pregnant animals were kept in a room tempreture with normal light and dark cycles and fed with normal diet. After injections to sham and treated groups for 15 days, 18 male offsprings were housed in three groups (6 placebo; 6 control; 6 treated). The 20 weeks-old animals were perfused and the prostates removed and embedded in paraffin. Tissue sections at 5μm thickness were taken at regular intervals and stained with Hematoxylin-eosin and Masson trichrome. Cavalieri principle was used for volume fractions analysis. Stereological examination revealed 55% acinar epitelium and 45% connective tissue in both control and placebo groups, as well as 60% acinar epitelium and 40% connective tissue in the treated group. Prenatally applied diclofenac sodium did not cause a statistically significant change in the prostate stroma parenchymal ratio in 20-week old rats compared to the control animals (p>0.05).

Keywords:

Diclofenac sodium, Prenatal term, Rat, Prostate, Stereology,

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