Irfan AFZAL MUGHAL, Noor-ul-ain IRFAN, Saira SAFDAR, Sadia MOAZZAM, Asma IRFAN, Amna FAROOQİ, Muhammad ISMAİL

Association of CYP24A1 gene, Vitamin D deficiency and heart diseases in Pakistani patients

Background &Aims To analyze the association of CYP24A1 gene polymorphism with vitamin D deficiency and its related cardiovascular disorders in the Pakistani population. Materials and Methods The present prospective cohort study was carried out at KRL and Rawalpindi General Hospital from January 2017 to December 2018. A total of 110 subjects suffering from heart diseases like hypertension, myocardial infarction, or congestive cardiac failure (age range 12-90 years) were enrolled in this study with informed consent. Sampling was done by non-probability convenience sampling. Blood 24Hydroxyvitmin D levels were assessed in the participants by using an electrochemiluminescence system. Genetic polymorphism in the CYP24A1 gene was screened in selected patients (n=15) through PCR-RFLP, after genomic DNA extraction from the whole blood. Data were analyzed using SPSS version 24 for Mac. Fisher’s exact test and chi-square tests were applied for qualitative variables. The risk of polymorphism in CYP24A1(SNP rs6013897) genotypes TT, AT, and AA was determined by calculating the odds ratio and confidence interval. P-value

Keywords:

vitamin D deficiency, cardiovascular diseases, CYP24A1 gene,

PDF

___

1. Jorge A, Cordeiro J, Rosa M, Bianchi D. Vitamin D Deficiency and Cardiovascular Diseases. International Journal of Cardiovascular Sciences. 2018;31(4):2
2. Latic N, Erben RG. Vitamin D and cardiovascular disease, with emphasis on hypertension, atherosclerosis, and heart failure. International Journal of Molecular Sciences. 2020 Jan;21(18):6483.
3. Riaz H, Finlayson A, Bashir S, Hussain S, Mahmood S, Malik F et al. Prevalence of Vitamin D deficiency in Pakistan and implications for the future. Expert Review of Clinical Pharmacology. 2016;9(2):329-338.
4. Krist AH, Davidson KW, Mangione CM, Cabana M, Caughey AB, Davis EM, Donahue KE, Doubeni CA, Epling JW, Kubik M, Li L. Screening for Vitamin D Deficiency in Adults: US Preventive Services Task Force Recommendation Statement. JAMA. 2021 Apr 13;325(14):1436-42.
5. de la Guía-Galipienso F, Martínez-Ferran M, Vallecillo N, Lavie CJ, Sanchis-Gomar F, Pareja-Galeano H. Vitamin D and cardiovascular health. Clinical Nutrition. 2020 Dec 29.
6. Klomp SD, Manson ML, Guchelaar HJ, Swen JJ. Phenoconversion of cytochrome P450 metabolism: a systematic review. Journal of Clinical Medicine. 2020 Sep;9(9):2890.
7. Jayaraj JM, Kuriakose BB, Alhazmi AH, Muthusamy K. Structural and functional insights on vitamin D receptor and CYP24A1 deleterious single nucleotide polymorphisms: A computational and pharmacogenomics perpetual approach. Cell Biochemistry and Function. 2021 Oct;39(7):874-85.
8. Krasniqi E, Boshnjaku A, Wagner KH, Wessner B. Association between Polymorphisms in Vitamin D Pathway-Related Genes, Vitamin D Status, Muscle Mass and Function: A Systematic Review. Nutrients. 2021 Sep;13(9):3109.
9. Divanoglou N, Komninou D, Stea EA, Argiriou A, Papatzikas G, Tsakalof A, Pazaitou-Panayiotou K, Georgakis MK, Petridou E. Association of Vitamin D Receptor Gene Polymorphisms with Serum Vitamin D Levels in a Greek Rural Population (Velestino Study). Lifestyle Genomics. 2021 Jun 17:1-0.
10. Bao Q, Wang D, Zhang Y, Bao L, Jia H. The impact of CYP24A1 polymorphisms on hypertension susceptibility. Kidney and Blood Pressure Research. 2020;45(1):28-37.
11. Neves JPR , Silva AS, Morais LC, Diniz AS, Costa MJ, et al. Concentrações de 25-hidroxivitamina D e níveis pressóricos em idosos hipertensos. Arq Bras Endocrinol Metabol. 2012; 56(7):415-22. PMID:23108745
12. Milazzo V, De Metrio M, Cosentino N, Marenzi G, Tremoli E, Vitamin D and acute myocardial infarction. Word J Cardiol. 2017;;9(1):14-20. Doi: 10.4330/wje.v9.i1.14
13. Qian P, Cao X, Xu X, Duan M, Zhang Q, Huang G. Contribution of CYP24A1 variants in coronary heart disease among the Chinese population. Lipids in health and disease. 2020 Dec;19(1):1-7.
14. Van Ballegooijen AJ, Kestenbaum B, Sachs MC, de Boer IH, Siscovick DS, Hoofenagle AN, et al. Association of 25-hydroxyvitamin D and parathyroid hormone with incident hypertension: MESA (Multi-Ethnic Study of Atherosclerosis). J Am Coll Cardiol. 2014; 63(12):1214-22.
15. De Metrio M, Milazzo V, Rubino M, Cabiesti A Moltrasio M, Marana I, et al. Vitamin D plasma levels and in-hospital and 1-year outcomes in acute coronary syndromes: a prospective study. Medicine(Baltimore). 2015;94(19):e857
16. Lee JH, Gadi R, Spertus JA, Tang F,O’Keefe JH. Prevalence of vitamin D deficiency in patients with acute myocardial infarction. Am J Cardiol. 2011;107(11):1636-8. Doi:10.1016/j.amjcard.2011.01.048 [ Links ]
17. Milazzo V, De Metrio M, Cosentino N, Marenzi G, Tremoli E, Vitamin D and acute myocardial infarction. Word J Cardiol. 2017;;9(1):14-20. Doi: 10.4330/wje.v9.i1.14 [ Links ]
18. Pandit A, Mookadam F, Boddu S, Aryal Pandit A, Tandar A, Chaliki H, et al. Vitamin D levels and left ventricular diastolic function. 2014. Open Heart. 2014;1(1):e000011 Doi: 10.1136/opener-2013-000011.eCollection2014 [ Links ]
19. Kouvari M, Panagiotakos DB, Chrysohoou C, Yannakoulia M, Georgousopoulou EN, Tousoulis D, Pitsavos C, ATTICA study Investigators. Dietary vitamin D intake, cardiovascular disease, and cardiometabolic risk factors: a sex‐based analysis from the ATTICA cohort study. Journal of Human Nutrition and Dietetics. 2020 Oct;33(5):708-17.
20. Latic N, Erben RG. Vitamin D and cardiovascular disease, with emphasis on hypertension, atherosclerosis, and heart failure. International Journal of Molecular Sciences. 2020 Jan;21(18):6483.
21. Gouni-Berthold I, Berthold HK. Vitamin D and vascular disease. Current vascular pharmacology. 2021 May 1;19(3):250-68.
22. Rodriguez AJ, Mousa A, Ebeling PR, Scott D, De Courten B. Effects of vitamin D supplementation on inflammatory markers in heart failure: a systematic review and meta-analysis of randomized controlled trials. Scientific reports. 2018 Jan 18;8(1):1-8.
23. Gouni-Berthold I, Berthold HK. Vitamin D and vascular disease. Current vascular pharmacology. 2021 May 1;19(3):250-68
24. Kim DH, Meza CA, Clarke H, Kim JS, Hickner RC. Vitamin D and endothelial function. Nutrients. 2020 Feb;12(2):575.

Journal of Experimental and Clinical Medicine-Cover

Yayın Aralığı: Yılda 4 Sayı
Başlangıç: 1980
Yayıncı: Ondokuz mayıs Üniversitesi Tıp Fakültesi

Arşiv