Nasser HAGHİGHAT, Fulya BASMACI, Asiye Nehir ÖZDEN, Ibtisam AL-HASHİMİ

Assessment of Salivary Pellicle on the Surface of Dental Alloys, in vivo

Objectives: The molecular composition of the salivary pellicle adsorbed on the surface of dental alloys is determined by the chemical structure of the alloy. The purpose of this study is to compare the composition of salivary pellicle on two dental alloys, chrome cobalt (Co-Cr) and nickel chromium (Ni-Cr) surfaces.Materials and Methods: Protein profile of 2 hour salivary pellicle on customized Co-Cr and Ni-Cr palatal plates were kept in the mouth of healthy volunteers for two hours. Pellicle was used to compare the parotid and the whole saliva using SDS-PAGE (sodiumdodecylsulphate-poyacrylamide gel electrophoresis) and Western blot analysis.Results: Only a limited number of salivary proteins was observed in both Co-Cr and Ni-Cr pellicle. In addition, there were differences in proteins observed Co-Cr pellicle vs. Ni-Cr pellicle.Conclusion: The result of this study suggest there is selectivity in salivary protein adsorption to Co-Cr and Ni-Cr dental prostheses.

PDF

___

1. Glickman I. Clinical Periodontology. Clinical P. Philadelphia-London: W. B. Saunders Co; 1972. 290–311 p.
2. Ericson T MK. Saliva, formation, composition and possible role. In: Thylstrup A, Fejerskov O, editors. Textbook of Cariology. Copenhagen: Munksgaard; 1986. 28–32 p.
3. Edgerton M, Tabak LA, Levine MJ. Saliva: a significant factor in removable prosthodontic treatment. J Prosthet Dent 1987;57:57–66. [CrossRef ]
4. Edgerton M, Levine MJ. Biocompatibility: its future in prosthodontic research. J Prosthet Dent 1993;69:406–415. [CrossRef ]
5. Al-Hashimi I, Levine MJ. Characterization of in vivo salivary-derived enamel pellicle. Arch Oral Biol 1989;34:289–295. [CrossRef ]
6. Gibbons RJ. Bacterial adhesion to oral tissues: a model for infectious diseases. J Dent Res 1989;68:750–760. [CrossRef ]
7. Ueda A. Adsorption of salivary proteins and serum proteins to titanium. J Osaka Dent Univ 1998;32:59–65.
8. Ozden AN, Haghighat N, Al-Hashimi I. Preliminary evaluation of salivary pellicle on nickel-chromium alloy in vivo. Quintessence Int 2002;33:731–735.
9. Olsson J, van der Heijde Y, Holmberg K. Plaque formation in vivo and bacterial attachment in vitro on permanently hydrophobic and hydrophilic surfaces. Caries Res 1992;26:428–433. [CrossRef ]
10. Shahal Y, Steinberg D, Hirschfeld Z, Bronshteyn M, Kopolovic K. In vitro bacterial adherence onto pellicle-coated aesthetic restorative materials. J Oral Rehabil 1998;25:52–58. [CrossRef ]
11. Lee SJ, Kho HS, Lee SW, Yang WS. Experimental salivary pellicles on the surface of orthodontic materials. Am J Orthod Dentofacial Orthop 2001;119:59–66. [CrossRef ]
12. Yoo J-H, Kho H-S, Kim Y-K, Lee S-W, Chung S-C. Experimental salivary pellicles formed on the surface of self-curing resin. J Oral Rehabil 2003;30:251–259. [CrossRef ]
13. Smith PK, Krohn RI, Hermanson GT, et al. Measurement of protein using bicinchoninic acid. Anal Biochem 1985;150:76–85. [CrossRef ]
14. Steinberg D, Mor C, Dogan H, Zacks B, Rotstein I. Effect of salivary biofilm on the adherence of oral bacteria to bleached and non-bleached restorative material. Dent Mater 1999;15:14–20. [CrossRef ]
15. Steinberg D, Eyal S. Early formation of Streptococcus sobrinus biofilm on various dental restorative materials. J Dent 2002;30:47–51. [CrossRef ]
16. Teughels W, Van Assche N, Sliepen I, Quirynen M. Effect of material characteristics and/or surface topography on biofilm development. Clin Oral Implants Res 2006;17 Suppl 2:68–81. [CrossRef ]
17. Zhang N, Melo M, Weir M, Reynolds M, Bai Y, Xu H. Do Dental Resin Composites Accumulate More Oral Biofilms and Plaque than Amalgam and Glass Ionomer Materials? Mater (Basel, Switzerland) 2016;9:888. [CrossRef ]
18. Mutahar M, O’Toole S, Carpenter G, Bartlett D, Andiappan M, Moazzez R. Reduced statherin in acquired enamel pellicle on eroded teeth compared to healthy teeth in the same subjects: An in-vivo study. PLoS One 2017;12:e0183660. [CrossRef ]
19. Ventura TM da S, Cassiano L de PS, Souza E Silva CM de, et al. The proteomic profile of the acquired enamel pellicle according to its location in the dental arches. Arch Oral Biol 2017;79:20–29. [CrossRef ]
20. Meckel AH. The formation and properties of organic films on teeth. Arch Oral Biol 1965;10:585–598. [CrossRef ]
21. Hay DI. The isolation from human parotid saliva of a tyrosine-rich acidic peptide which exhibits high affinity for hydroxyapatite surfaces. Arch Oral Biol 1973;18:1531–1541. [CrossRef ]
22. Hay DI. Fractionation of human parotid salivary proteins and the isolation of an histidine-rich acidic peptide which shows high affinity for hydroxyapatite surfaces. Arch Oral Biol 1975;20:553–558. [CrossRef ]
23. Hannig C, Hannig M. The oral cavity--a key system to understand substratum-dependent bioadhesion on solid surfaces in man. Clin Oral Investig 2009;13:123–139. [CrossRef ]
24. Göcke R, Gerath F, von Schwanewede H. Quantitative determination of salivary components in the pellicle on PMMA denture base material. Clin Oral Investig 2002;6:227–235. [CrossRef ]
25. Kohavi D, Klinger A, Steinberg D, Sela MN. Adsorption of salivary proteins onto prosthetic titanium components. J Prosthet Dent 1995;74:531–534. [CrossRef ]
26. Amerongen N, Oderkerk CH, Driessen AA. Role of mucins from human whole saliva in the protection of tooth enamel against demineralization in vitro. Caries Res 1987;21:297–309. [CrossRef ]
27. Denny P, Hagen FK, Hardt M, et al. The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions. J Proteome Res 2008;7:1994–2006. [CrossRef ]
28. Edgerton M, Levine MJ. Characterization of acquired denture pellicle from healthy and stomatitis patients. J Prosthet Dent 1992;68:683–691. [CrossRef ]
29. Scannapieco FA, Torres GI, Levine MJ. Salivary amylase promotes adhesion of oral streptococci to hydroxyapatite. J Dent Res 1995;74:1360–1366. [CrossRef ]
30. Carlén A, Rüdiger SG, Loggner I, Olsson J. Bacteria-binding plasma proteins in pellicles formed on hydroxyapatite in vitro and on teeth in vivo. Oral Microbiol Immunol 2003;18:203–207. [CrossRef ]
31. Singh AV, Vyas V, Patil R, et al. Quantitative characterization of the influence of the nanoscale morphology of nanostructured surfaces on bacterial adhesion and biofilm formation. PLoS One 2011;6:e25029. [CrossRef ]
32. Wang Z, Shen Y, Haapasalo M. Dental materials with antibiofilm properties. Dent Mater 2014;30;e1–e16. [CrossRef ]
33. Hasan J, Crawford RJ, Ivanova EP. Antibacterial surfaces: the quest for a new generation of biomaterials. Trends Biotechnol. 2013;31:295–304. [CrossRef ]