The aim of this study was to investigation the effects of in ovo administration of glucose on hatching traits and blood glucose concentration in newly- hatched chicks. Six hundred fertile eggs were divided to four groups; 1) control (without injection), 2) group including 0.5 ml deionized water (sham group), 3) group including 0.5 ml glucose %15 in deionized water, 4) group including 0.5 ml glucose %20 in deionized water with three replicates per treatment and 50 eggs per replicate. After hatching, hatching traits and blood glucose concentration was determined. Results showed that group glucose %15 and group glucose %20 as compared with groups control and sham group had significantly higher blood glucose concentration and the weight of newly-hatched chicks. Control group than other groups had significantly higher percent of hatching, but there were no difference between experimental groups of glucose injection and sham group. Data Suggest, in ovo injection can be reduced hatchability and increase mortality in hatch period