In this study, the morphological and anatomical features of Mitrophora semilibera (DC.) Lev. spores and mycelium were examined. In our country; they show a wide distribution in temperate periods. Cap is reach diameters up to 2-5 cm, brownish, conical and irregular brownish ribs. Stalk is whitish, about 9-10 cm long and hollow. The mushroom samples were collected from Kırıkkale region at Turkey and were brought to the laboratory. Tissue fragments were taken and they were cultured on the potato dextrose agar (PDA). They were incubated in the dark for 10 days, in 24°C. In the during incubation period, the development of mycelium were measured on a daily and the radial growth rates were taken as criteria. During the development, the mycelium has cottony to the agar medium surface and they were so quickly developed. There were air hyphae. No pigmentation was observed at the mycelium. The spores and mycelium of M. semilibera were investigated with help of both light microscopy and scanning electron microscopy (SEM). The spores of M. semilibera are smooth, with dimensions of 22-28 x 11–16 µm. Generally, they shaped like elliptical