In this study, in order to define how effective one alcohol based ground and surface disinfectant with three –acid characters is to which concentration against Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 23853), Candida albicans (ATCC 10231) strains used for Test Microorganisms, final microorganisms was disinfectant into the different concentrations of the disinfectant to be tested by being prepared as 2-5×109 CFU/ml. according to the McFarland 5 cloudiness of Test disinfectant. After disinfectants were activated with microorganisms at previously experimented 1.5, 15 and 30 minute-periods, colony counts at 1 ml. levels were performed by way of cast-cultural plaque method. Consequently, it was determined that 1% concentration of alcohol based ground and surface disinfectant influenced Staphylococcus aureus strain at the rate of 0.5% in 1 min., and other concentrations over 1% influenced other test microorganisms in 1 minute. It was also determined that 0.1% and over percent concentrations of the disinfectant containing peracetic acid were effective against Staphylococus aureus in 1 minute, while other test microorganisms were effective in 1 minute at 0.5% and over percent concentrations