Globulin and Albumin blood-serum proteins of 48 Ellobius specimens which were collected from north-east (Mashhad and Bojnord), west (Kurdistan, Hamadan, Qazvin, and Zanjan), and north-west (Pir-ahmad-kandi, Kelisa-kandi, and Nadou villages) of Iran have been examined using SDS-PAGE method. In residual region of Globulin protein (G), specimens from north-east and west group and north-west group had created 5 and 11 electrophoretic bands, respectively; and in residual region of Albumin protein (A) all specimens created only one band and north-west group created 4 electrophoretic bands. Although these electrophoretic band differences could led to diagnosis of these two Ellobius specimens, but this difference along with morphological and Karyological properties of these specimens, can help identifying the distribution of Ellobius fuscocapillus in north-east and west and Ellobius lutescens in north-west of the Iran