The aim of this study was to evaluate the antibacterial activity such as time-kill study of the butanol extract from Urtica dioica against methicillin-resistant Staphylococcus aureus (MRSA) using standard microbiological procedures. The effects of the butanol extract on MRSA cells was also studied by means of scanning electron microscope (SEM) and the results showed that the butanol extract caused internal shrinkage of the cells and completely collapsed the cells after prolonged exposure to it. Minimum Inhibitory Concentration (MIC) value was 16.67 mg/mL. The average log reduction in viable cell count in time-kill assay was > 4.5 log10 CFU/mL using 33.33 mg/mL (2MIC) for 48 h.The extract was bactericidal against MRSA at 2MIC from 44 h interaction period. However, the time-killed assay suggested that the butanol extract of U. dioica significantly inhibited the cell growth and possessed bactericidal activity at concentration