Ashok K. SHAKYA, Shankar KATEKHAYE, Ghaleb A. ORIQUAT, Rajashri R. NAIK, Rajashri R. NAIK, Anant PARADKAR, Hugo FEARNLEY, James FEARNLEY

Fatty Acid Analysis and Biological Activity of Jordanian Propolis

Propolis is a resinous natural product collected by bees (Apis mellifera) from tree exudates which is widely used in folk medicine1. Reports on Jordanian Propolis reveal the presence of new chemical compound 4(Z)-1-3-dihydroxyeupha-7,24-dien-26-oic acid2 along with other compounds like pinobanksin-3-O-acetate, pinocemberin, chrysin3 and lignoceric acid2. The present study was carried out to investigate the fatty acid composition, antioxidant and xanthine oxidase inhibition activity of Jordanian Propolis, collected from Al-Ghour region. The hexane extract of Jordanian Propolis contains different fatty acids, which are reported first time, using GC-FID. The major fatty acid identified were palmitic acid (44.5%), Oleic acid (18:1∆9cis, 24.6%), Arachidic acid (7.4%), Stearic acid (5.4%), linoleic acid (18:2∆9-12cis, 3.1%), caprylic acid (2.9%), lignoceric acid (2.6%), cis-11,14-eicosadienoic acid (20:2∆11-14cis, 2.4%), palmitoleic acid (1.5%), cis-11-eicosenoic acid (1.2%), α–linolenic acid (18:3∆9-12-15cis, 1.1%), cis-13,16-docosadienoic acid (22:2∆13-16cis, 1.0%), along with minor constituents like saturated fatty acids. Antioxidant properties of the hexane extract were determined via DPPH radical scavenging, β-carotene bleaching assay and NO scavenging assay. The extract produced significant antioxidant activity in-vitro. The extract also exhibit appreciable xanthine oxidase inhibitory activity in-vitro.

Keywords:

Propolis, Naturel product, DPPH, In vitro,

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1. Toreti, V.C., Sato, H.H., Pastore, G.M. and Park, Y.K., 2013. Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evidence-based complementary and alternative medicine, 2013. http://dx.doi.org/10.1155/2013/697390
2. Shaheen, S.A., Zarga, M.H.A., Nazer, I.K., Darwish, R.M. and Al-Jaber, H.I., 2011. Chemical constituents of Jordanian propolis. Natural product research, 25(14), 1312-1318.
3. Darwish, R.M., Ra'ed, J., Zarga, M.H.A. and Nazer, I.K., 2010. Antibacterial effect of Jordanian propolis and isolated flavonoids against human pathogenic bacteria. African Journal of Biotechnology, 9(36). 5966-5974