Salvia verticillata L., Salvia tomentosa Mill. ve Phlomis lychnitis L.'nin Toplam Fenolik ve Flavonoid İçerikleri ve Antioksidan Aktivitesi

Çalışmamızın amacı, Salvia verticillata L., S. tomentosa Mill. ve Phlomis lychnitis L.'nin metanol ve etil asetat ekstraklarının toplam fenolik, toplam flavonoid ve antioksidan özelliklerini araştırmaktır. Ekstraktların antioksidan potansiyelleri DPPH (2,2-difenil-1-pikrilhidrazil) serbest radikal temizleme etkinliği kullanılarak belirlendi. Toplam fenolik içerik (TPC) ve toplam flavonoid içerik (TFC) spektrofotometrik yöntem kullanılarak değerlendirildi. S. verticillata’nın metanol ekstraktı, TPC ve TFC’de en yüksektir. Aynı ekstrakt, 0.01 ± 0.00 mg / mL SC50 değeri ile en iyi antioksidan aktivite ortaya koydu. Ayrıca DPPH ve TFC (r = 0.904; p <0.01) ve TPC (r = 0.963; p <0.01) arasında da güçlü pozitif korelasyon bulundu. Bu ilişki, flavonoidlerin diğer fenolik bileşiklerle birlikte bu biyoaktiviteye büyük katkıda bulunduğunu gösterdi. Sonuçlarımız ayrıca S. verticillata L.'nin metanol ekstraktının, ilaç ve gıda endüstrilerinde kullanım için yüksek kaliteli bir antioksidan olduğunu ileri sürmektedir.

Anahtar Kelimeler:

DPPH, toplam fenolik, toplam flavonoid, S. tomentosa Mill. Salvia verticillata L., Phlomis lychnitis L

Antioxidant Activity and Total Phenolic and Flavonoid Contents of Salvia verticillata L., Salvia tomentosa Mill., and Phlomis lychnitis L.

In this study, we investigated total phenolic, total flavonoid, and antioxidant properties of methanol and ethyl acetate extracts of Salvia vertisillate L., Salvia tomentosa, and Phlomis lychnitis L. Antioxidant potentials of the extracts were determined by (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity. The total phenolic content and total flavonoid content were evaluated by spectrophotometric method. Methanol extract of Salvia vertisillate was found to be the highest total phenolic and total flavonoid content. The same extract revealed the most significant antioxidant effect with SC50 value of 0.01±0.mg/mL. Strong positive correlations were also found between DPPH and total flavonoid content (R=0.904; p<0.01), total phenolic content (R=0.963; p<0.01). This relationship indicated that flavonoids contribute greatly to this bioactivity together with other phenolic compounds. Results indicate that the methanol extract of Salvia vertisillate L. proved to be a good sources for medicine and food industry as a natural antioxidant.

Keywords:

total flavonoid, total phenolic , Salvia tomentosa, , Salvia vertisillate L., DPPH,

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