Didem PEKMEZCİ, Gamze Nur KONAÇOĞLU, Zeynep Nurselin ÇOLAK

Kanin Distemper Virus ile Enfekte Köpeklerde Nötrofil/Lenfosit, Monosit/Lenfosit ve Platelet/Lenfosit Oranlarının Belirlenmesine Yönelik Bir Ön Çalışma

Sunulan ön çalışma ile doğal olarak Kanin Distemper Virüs (KDV) ile enfekte köpeklerin tedavi öncesi Nötrofil/Lenfosit (NEU/LYM), Monosit/Lenfosit (MONO/LYM), ve Platelet/Lenfosit (PLT/LYM) oranları ile tam kan sayımı (TKS) verilerin sağlıklı bireyler ile karşılaştırılması amaçlanmıştır. Bu amaçla retrospektif olarak Veteriner Fakültesi, Hayvan Hastanesi kayıtlarında yer alan 22 adet sahipli, 3-12 (6,2) aylık, KDV enfeksiyonu tanısı almış ve hızlı test kitleri ile pozitifliği saptanan köpekler çalışma grubunu oluşturmuştur. Diğer taraftan, 8 adet sahipli, 2-10 (8,2) aylık ve sağlıklı olduğu tespit edilen köpek ise kontrol grubunu oluşturmuştur. Gruplar arasında tedavi öncesi NEU/LYM, MONO/LYM, PLT/LYM ve TKS parametrelerinin karşılaştırılmasında KDV pozitif grubu medyan NEU/LYM, MONO/LYM, PLT/LYM ile TKS değerlerinden sadece LYM, EOS, %NEU, %LYM, %EOS, %BAS, MCH, MCHC, RDWCV, PLT değerleri arasında istatistiksel fark (P 0,05) bulunmuştur. KDV pozitif grubu medyan NEU/LYM, MONO/LYM ve PLT/LYM düzeyleri ise sırasıyla 31,9, 2,33 ve 391,2 olarak bulunurken kontrol grubunun aynı değerleri sırası ile 6,39, 0,37 ve 187,7 olarak tespit edilmiştir. KDV hastalarının tahmin edilmesinde aynı zamanda NEU/LYM, MONO/LYM ve PLT/LYM değerlerinin optimize edilmiş kesim noktaları ise sırası ile NEU/LYM =7,6, MONO/LYM=0,61 ve PLT/LYM=0,852 olarak tespit edilmiştir. Sonuç olarak, bu ön çalışma NEU/LYM, MONO/LYM ve PLT/LYM değerlerinin KDV ile enfekte köpeklerin şüpheli durumlarda tanılarının desteklenmesinde klinisyenlerce kullanılabilecek ucuz, objektif ve invazif olmayan yeni belirteç adayı olabileceklerini düşündürmektedir.

Anahtar Kelimeler:

Kanin Distemper Virüs, , Köpek, , Monositin lenfosite oranı, , Nötrofilin lenfosite oranı, , Plateletin lenfosite oranı

A Preliminary Study for Determination of Neutrophil to Lymphocyte, Monocyte to Lymphocyte and Platelet to Lymphocyte Ratios in Dogs with Canine Distemper Virus Infection

It is aimed to compare the pretreatment Neutrophil/Lymphocyte (NEU/LYM), Monocyte/Lymphocyte (MONO/LYM), and Platelet/Lymphocyte (PLT/LYM) ratios with whole blood count (WBC) of dogs naturally infected Canine Distemper Virus (CDV) with healthy individuals within this preliminary study. For this purpose, retrospectively, 22 owned, 3-12 (6.2) months old dogs diagnosed with CDV infection found to be positive with rapid test kits based up on the records of Animal Hospital, Faculty of Veterinary Medicine, constituted the study group. On the other hand, eight owned, 2-10 (8.2) months old, and healthy dogs enrolled in the control group. In the comparison of pre-treatment NEU/LYM, MONO/LYM, PLT/LYM, and WBC parameters between the groups, we found only a statistical difference (P 0.05) between median LYM, EOS, NEU%, LYM%, EOS%, BAS%, MCH, MCHC, RDWCV, PLT with NEU/LYM, MONO/LYM, PLT/LYM ratios. The median NEU/LYM, MONO/LYM, and PLT/LYM levels in the CDV positive group were 31.9, 2.33, and 391.2, respectively, while the same values of the control group were determined as 6.39, 0.37 and 187.7, respectively. The optimized cut-off points of NEU/LYM, MONO/LYM, and PLT/LYM values in estimating CDV patients were also determined as NEU/LYM =7.6, MONO/LYM=0.61 and PLT/LYM=0.852, respectively. In conclusion, this preliminary study suggests that NEU/LYM, MONO/LYM, and PLT/LYM values may be inexpensive, objective, and non-invasive new marker candidates for clinicians to support the diagnosis of CDV infected dogs in suspicious circumstances.

Keywords:

Canine Distemper Virus, , Dog, , Monocyte to Lymphocyte ratio, Neutrophil to Lymphocyte ratio, , Platelet to Lymphocyte ratio,

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