Başak BÜYÜK, Tuba DEMİRCİ, Neslihan DEMİR, Hakan TÜRKÖN

GANODERMA LUCIDUM SPERM MOTILITE ORANINI ARTTIRMAK İÇİN ALTERNATİF BİR BESLENME DESTEĞİ OLABİLİR Mİ?

Ganoderma lucidum (GL) yaygın olarak kullanılan tıbbi bir mantardır. Bu mantarın terapötik etkileri daha önceki birçok çalışmada kullanılmıştır. Bu çalışmanın amacı, düşük, orta ve yüksek doz uygulanan GL ekstresinin sıçanlarda testis dokusu, spermatogenic seri hücreleri ve sperm motilitesi üzerine etkisini araştırmaktır. Bu amaçla 40 adet Wistar albino cinsi erkek sıçan rastgele dört gruba ayrılmıştır. Grup 1’deki sıçanlara 2 ml serum fizyolojik, Grup 2, 3 ve 4’deki sıçanlara ise sırasıyla 500 mg/kg, 2500 mg/kg ve 5000 mg/kg Ganoderma lucidum ekstresi toplam dokuz gün ve günde bir kez gavaj yoluyla verilmiştir. Sperm motilitesi ve histopatolojik değişiklikleri değerlendirmek için epididimal sperm toplama işlemi yapıldı ve testis dokusundan örnek alındı. Kan örnekleri ise biyokimyasal değerlendirme için alındı. Grup 1 (Kontrol) grubu ile Grup 2, 3 ve 4 karşılaştırıldığında Johnsen skoru ve sperm motilitesi bu gruplarda artmış olarak bulundu ve bu artış istatistiksel olarak anlamlıydı. Sonuç olarak, uygulanan düşük, orta ve yüksek doz GL ekstresinin spermatogenezi, epididimal total sperm sayısını ve progresif motil sperm sayısını artırdığı ortaya konmuştur. Ancak, yüksek dozlar testis dokusunda minimal hasara neden olmuştur, ayrıca sperm parametrelerindeki yükselme anlamlı bulunmamıştır. Bu nedenle, 2500 mg/kg'ın üzerindeki oral kullanım dozlarından kaçınılması gerektiği sonucuna varılmıştır.

Anahtar Kelimeler:

Ganoderma lucidum, erkek infertilitesi, sperm motilitesi, testis histopatolojisi

CAN GANODERMA LUCIDUM BE AN ALTERNATIVE NUTRITIONAL SUPPLEMENT FOR ENHANCING SPERM MOTILITY RATE?

Ganoderma lucidum (GL) is a widely used medicinal mushroom. The therapeutic effect of this fungus on many diseases has been proven by studies. The aim of this study is to assess the effects of low, moderate and high dose GL extract administration on the testis tissue, spermatogenic series cells and sperm motility in rats. 40 Wistar albino rats were randomly divided into 4 groups. Rats in group 1, 2, 3 and 4 were administered 2 ml physiologic serum, 500 mg/kg, 2500 mg/kg, 5000 mg/kg Ganoderma lucidum extract 1 time per day via gavage for 9 days, respectively. For evaluation of sperm motility and histopathological changes, epididymal sperm collection and testis harvesting were done. Blood samples were collected for biochemical analysis. When the Group 1 (control group) is compared with Groups 2, 3 and 4, the Johnsen score and sperm motility in these groups increased and this increase was statistically significant. In conclusion, low, moderate, and high doses of GL extract administered to rats were revealed to increase spermatogenesis, epididymal total sperm count and progressive motile sperm counts. However, it is detected that high doses cause minimal damage to the testis and as the increase in sperm parameters wasn’t significant, it’s concluded that doses for oral use above 2500 mg/kg should be avoided.

Keywords:

Ganoderma lucidum, Male fertility, Sperm motility, Testis histopathology,

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