Burcu SÜMER TÜZÜN, Tuğçe FAFAL, Recep İLHAN, Bijen KIVÇAK, Petek BALLAR KIRMIZIBAYRAK

Antioxidant, tyrosinase inhibitor, and cytotoxic effects of Anthemis aciphylla Boiss. var. aciphylla and Cota dipsacea (Bornm.) Oberpr. & Greuter

Background and Aims: The study aims to examine the antioxidant , tyrosinase inhibitory, and cytotoxic activities of the methanol and chloroform extracts of Anthemis aciphylla Boiss. var. aciphylla and Cota dipsaceae (Bornm.) Oberpr. & Greuter in order to evaluate the results regarding different polarities. Methods: The study evaluates the total phenolic and total flavonoid content of the extract sand the in vitro antioxidant activities using the DPPH, ABTS, superoxide radical, and nitric oxide scavenging activities. Tyrosinase inhibitor activity was identified by Masuda’s method. Cytotoxicity was investigated using the MTT assay. Results: The A. aciphylla var. aciphylla methanol extract (AAM) was the most active extract in term of overall antioxidant activity. The IC50 values for DPPH, ABTS, and superoxide anion radical activity were found to be 19.57, 46.82, and 5.610 µg/ mL, respectively. Nitric oxide scavenging activity percent inhibition of the AAM was 46.47%. The tyrosinase inhibitory activity of AAM, was also the most effective, thus confirming the study’s hypothesis. Moreover, no cytotoxic activity was observed to be present in the extracts with biological activity. Conclusion: According to the hypothesis, the tyrosinase inhibitor effect should be observed mostly in extracts and compounds that show the high antioxidant activity mostly originating from phenolic substances. AAM confirmed this hypothesis as the most active extract regarding both the tyrosinase inhibitor effect as well as antioxidant activities. Furthermore, the MTT assay was used to find AAM to be non-cytotoxic at the tested concentrationsThis indicates AAM to be able to be used more reliably in medicine and as a food preservative.

Keywords:

Anthemis, Cota, antioxidant activity, cytotoxic activity, tyrosinase inhibitor activity,

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