Türkiye'den Fumaria asepala Boiss. ve Fumaria schleicheri Soy.-Will. subsp. microcarpa Hausskn. 'nın Uçucu Yağ Bileşenleri ile Antimikrobiyal Aktivitesi ve Antioksidan Kapasitesinin Analizi

Bu çalışmada, Türkiye’de yetişen Fumaria asepala Boiss. (Akşahtere) ve Fumaria schleicheri Soy.-Will. subsp. microcarpa Boiss. ex Hausskn. (Şahtere) türlerinin uçucu yağ bileşenleri ile metanol ekstrelerinin antimikrobiyal ve antioksidan aktivitelerinin belirlenmesi amaçlanmıştır. Uçucu yağlar hidrodistilasyon yöntemi ile izole edilirken, bu yağların analizi GC/MS cihazı ile belirlenmiştir. Elde edilen sonuçlara göre F. asepala’nın uçucu yağının esas bileşeninin Phytol (%20.74) olduğu, bunu Thymol (%20.42), Benzyl Benzoate (%15.89) ve Hexahydrofarnesyl acetone (%12.92)’ ın izlediği; F. schleicheri subsp. microcarpa’nın ise uçucu yağının esas bileşeninin Benzyl Benzoate (%29.07) olduğu ve bunu sırasıyla Hexahydrofarnesyl acetone (%19.72), n-Hexadecanoic acid (%11.40) ve Phytol (%10.04)’ ün izlediği saptanmıştır. Antimikrobiyal etkilerini tespit etmek için disk difüzyon yöntemi kullanılmıştır. F. schleicheri subsp. microcarpa’nın toprak üstü kısımları en iyi antimikrobiyal etkiyi K. pneumoniae’e- ATCC 700603 (25mm) karşı gösterirken, F. asepala ise en iyi antimikrobiyal etkiyi S. aureus-ATCC 25923 (24mm) ve K. pnuemoniae’e karşı (24mm) göstermiştir. Antioksidan etkilerini belirlemek için toplam antioksidan seviyesi (TAS) ve toplam oksidan seviyesi (TOS) tespit edilmiştir. F. schleicheri subsp. microcarpa’nın 1 mg/ml konsantrasyonda metanol ekstresinin TAS değeri 2.8314 mmol olduğu, F. asepala’nın TAS değerinin aynı konsantrasyonda 3.1610 mmol olduğu tespit edilmiştir. Her iki türün toplam oksidan seviyelerinin ise yüksek değerlerde olduğu belirlenmiştir.

Anahtar Kelimeler:

Antimikrobiyal aktivite, antioksidan kapasite, fumaria, uçucu yağ

Analysis of the Essential Oil Composition, Antimicrobial Activity and Antioxidant Capacity of Fumaria asepala Boiss. and Fumaria schleicheri Soy.Will. subsp. microcarpa Hausskn. from Turkey

This study aims to determine the antimicrobial and antioxidant activities of essential oil components and methanol extracts of Fumaria asepala Boiss. (Akşahtere) and Fumaria schleicheri Soy.-Will. subsp. microcarpa Hausskn. (Şahtere) species grown in Turkey. While essential oils were isolated by the hydrodistillation method, the analysis of these oils was determined by GC/MS device. According to the results obtained, the main component of the essential oil of F. asepala is Phytol (20.74%), followed by Thymol (20.42%), Benzyl Benzoate (15.89%), and Hexahydrofarnesyl acetone (12.92%); It was determined that the main component of the essential oil of F. schleicheri subsp. microcarpa is Benzyl Benzoate (29.07%), followed by Hexahydrofarnesyl acetone (19.72%), n-Hexadecanoic acid (11.40%) and Phytol (10.04%). The disk diffusion method was used to determine its antimicrobial effects. The above-ground parts of F. schleicheri subsp. microcarpa showed the best antimicrobial effect against K. pneumoniae-ATCC 700603 (25mm), while F. asepala showed the best antimicrobial effect against S. aureus-ATCC 25923 (24mm) and K. pneumoniae (24mm). To determine their antioxidant effects, total antioxidant level (TAS) and total oxidant level (TOS) were determined. It was determined that the TAS value of the methanol extract of F. schleicheri subsp. microcarpa at a concentration of 1 mg/ml was 2.8314 mmol, and the TOS value of F. asepala was 3.1610 mmol at the same concentration. It was determined that the total oxidant levels in both species were high.

Keywords:

Antimicrobial activity, Antioxidant capacity, Fumaria, Essential oil,

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