Intravenous cannula can increase serum creatine kinaseMB activity

Objectives: Different blood sampling methods may result in differing creatine kinase MB (CK-MB) results. The aim of this study was to assess the effect of 2 phlebotomy methods on the CK-MB level in serum samples of healthy adults.Methods: The study used 50 volunteers (20 females, 30 males) who were employees at Baskent University Hospital in Ankara, Turkey. The subjects were randomly assigned to the study group. Blood samples were collected with the most widely used equipment for each method being tested (20G intravenous [IV] cannula and 20G needle unit) to compare the effects of different methods of blood sampling. The mean values of CK-MB and creatine kinase (CK) activity were compared using a paired samples t-test.Results: The mean CK-MB activity was 14.4±4.1 U/L and the mean CK activity was 127.3±17.1 U/L in samples that were drawn with a 20G needle unit, while in samples drawn with a 20G IV cannula, the corresponding results were 19.3±3.8 U/L and 132.5±16.1 U/L (p

Kaynakça

1. Gach O, El HZ, Lancellotti P. Acute coronary syndrome. [Article in French] Rev Med Liege 2018;73:243–50.

2. Akhmedova DI, Akhmedova NR, Aripov AN, Matkarimova AA. Biochemical Markers of Myocardium Damage in Children With Cardiomyopathy. [Article in Russian] Klin Lab Diagn 2019;64(6):337–41.

3. Lowe G, Stike R, Pollack M, Bosley J, O'Brien P, Hake A, et al. Nursing blood specimen collection techniques and hemolysis rates in an emergency department: analysis of venipuncture versus intravenous catheter collection techniques. J Emerg Nurs 2008;34(1):26–32.

4. Chang CC, Liou CB, Su MJ, Lee YC, Liang CT, Ho JL, et al. Creatine Kinase (CK)-MB-to-Total-CK Ratio: a Laboratory Indicator for Primary Cancer Screening. Asian Pac J Cancer Prev 2015;16(15):6599–603.

5. Zhang JQ, Yang QY, Xue FS, Zhang W, Yang GZ, Liao X, et al. Preoperative oral thyroid hormones to prevent euthyroid sick syndrome and attenuate myocardial ischemia-reperfusion injury after cardiac surgery with cardiopulmonary bypass in children: A randomized, double-blind, placebo-controlled trial. Medicine (Baltimore) 2018;97(36):e12100.

6. Kirschner MB, Kao SC, Edelman JJ, Armstrong NJ, Vallely MP, van Zandwijk N, et al. Haemolysis during sample preparation alters microRNA content of plasma. PLoS One 2011;6(9):e24145.

7. Kwon HJ, Seo EJ, Min KO. The influence of hemolysis, turbidity and icterus on the measurements of CK-MB, troponin I and myoglobin. Clin Chem Lab Med 2003;41(3):360–4.

8. Pateghini M, Renze B. Serum Enzymes. In: Burtis CA, Burns DE, editors. Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. 7th ed. Missouri: Elsevier Saunders; 2015. p. 320.

9. Pateghini M, Renze B. Serum Enzymes. In: Burtis CA, Burns DE, eds. Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. 7th ed. Missouri: Elsevier Saunders; 2015. p 321– 2.

10. Thomas L. Haemolysis as Influence & Interference Factor. EJIFCC 2002;13(4):95–8.

11. Lippi G, Blanckaert N, Bonini P, Green S, Kitchen S, Palicka V, et al. Haemolysis: an overview of the leading cause of unsuitable specimens in clinical laboratories. Clin Chem Lab Med 2008;46(6):764–72.

12. Haverstick DM, Grosbach AR. Specimen Collection and Processing. In: Burtis CA, Ashwood ER, Bruns DE, editors. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 5th ed. Missouri: Elsevier Saunders; 2012. p. 146–8.

13. Horder M, Elser RC, Gerhardt W, Mathieu M, Sampson EJ. International Federation of Clinical Chemistry. Scientific Division, Committee on Enzymes. IFCC methods for the measurement of catalytic concentration of enzymes. Part 7. IFCC method for creatine kinase (ATP: creatine N-phosphotransferase, EC 2.7.3.2). IFCC recommendations. Clin Chim Acta 1990;190(1- 2):S4–S40.

14. Neumeier D, Prellwitz W, Würzburg U, Brundobler M, Olbermann M, Just H-J, et al. Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity. Activity kinetics and diagnostic significance in myocardial infarction. Clin Chim Acta 1976;73(3):445–51.

15. Higgins T, Eckfeldt JH, Barton JC, Doumas BT. Hemoglobin, Iron, and Bilirubin. In: Burtis CA, Ashwood ER, Bruns DE, editors. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 5th ed. Missouri: Elsevier Saunders; 2012. p. 987.

16. Panteghini M, Renze B. Serum Enzyms. In: Burtis CA, Ashwood ER, Bruns DE editors. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 5th ed. Missouri: Elsevier Saunders; 2012. p. 569–71.

17. Dasgupta A, Wells A, Biddle DA. Negative interference of bilirubin and hemoglobin in the MEIA troponin I assay but not in the MEIA CK-MB assay. J Clin Lab Anal 2001;15:76–80.

18. Gunduz H, Arinc H, Yolcu M, Akdemir R, Kanat M, Uyan C. A case of hypothyroidism mimicking acute coronary syndrome. Int J Cardiovasc Imaging 2006;22(2):141–5.

19. Beck SR. Myocardial injury in critically ill patients. JAMA 1995;274(21):1672–3.

20. Lippi G, Avanzini P, Cervellin G. Prevention of hemolysis in blood samples collected from intravenous catheters. Clin Biochem 2013;46(7-8):561–4.

21. Panteghini M, Renze B. Serum Enzyms. In: Burtis CA, Ashwood ER, Bruns DE, editors. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 5th ed. Missouri: Elsevier Saunders; 2012. p. 571.

Kaynak Göster