Biological effects of Beauveria bassiana and Akanthomyces attenuatus isolates on Aphis gossypii Glover (Hemiptera: Aphididae)

The biological effects of entomopathogenic species, Beauveria bassiana (Bals.-Criv.) Vuill and Akanthomyces attenuatus Zare & Gams on cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae) nymphs at 1x108 conidia concentration (ml-1) were investigated at the laboratory conditions. The experiments were conducted at 25°C, 65% relative humidity, and 16:8 hours of lighting in a climatic cabinets. Alive nymphs were recorded on the 1st, 3rd, 5th, 7th, and 9th days of incubation. B. bassiana caused higher mortality of the nymphs than A. attenuatus did. A statistical difference was determined between the isolates on the 7th and 9th days of the experiment. The highest mortality rates were determined in the isolate of B. bassiana with 72% and the isolate of A. attenuatus with 54% on the 9th day. The LT50 value for the isolates of B.bassiana and A. attenuatus was 6.02 days and 8.33 days, respectively.

Keywords:

Beauveria bassiana, , Akantomyces attenuatus entomopathogenic fungus, , biological control,

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Abbott, W.S. (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267.
Anonymous. (2023). Investigation on the effect of four isolates of Beauveria bassiana (Balsamo) on the melon aphid on greenhouse cucumber under laboratory conditions. https://agris.fao.org/agris-search/search.do?recordID=IR2011003090
Akbari, S., Safavi, S.A., Ghosta, Y. (2013). Efficacy of Beauveria bassiana (Blas.) Vuill. against cabbage aphid Brevicoryne brassicae L. (Hem.: Aphididae) in laboratory condition. Archives of Phytopathology and Plant Protection, 47(12), 1454-1458. https://doi.org/10.1080/03235408.2013.845972
Ainsworth, G.C., Bisby, G.R., Kirk, P.M. (2008). Ainsworth & Bisby’s Dictionary of the Fungi, 5th ed.; CABI: Wallingford, UK, 771p.
Basu, M., Patro, B. (2007). New records of host plants and natural enemies of Aphis gossypii Glover (Aphididae: Homoptera) from Orissa, India. Journal of Plant Protection and Environment, 4(2): 74–80.
Behi, F., Souissi, R., Boukhris-Bouhachem, S. (2019). Temporal changes in the aphid–natural enemy complex in Tunisian citrus over two decades. Journal of Entomological Science, 54(4): 357–369. https://doi.org/10.18474/JES18-97
Blackman, R.L., Eastop, V.F. (2000). Aphids on the World’s Crops: An identification and information guide. The Natural History Museum. John Wiley and Sons, Ltd., New York, 476p.
de Faria, M.R., Wraight, S.P. (2007). Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types. Biological Control, 43(3): 237–256. https://doi.org/10.1016/j.biocontrol.2007.08.001
Guldemond, J.A., Tigges, W.T., De Vrijer, P.W. (1994). Host races of Aphis gossypii (Homoptera: Aphididae) on cucumber and chrysanthemum. Environmental Entomology, 23(5): 1235–40. https://doi.org/10.1093/ee/23.5.1235
Gurulingappa, P., McGee, P.A., Sword, G. (2011). Endophytic Lecanicillium lecanii and Beauveria bassiana reduce the survival and fecundity of Aphis gossypii following contact with conidia and secondary metabolites. Crop Protection, 30(3): 349-353. https://doi.org/10.1016/j.cropro.2010.11.017
Gül., E., Demirci, F., Babaroğlu, N. (2022). Characterization of Lecanicillium psalliotae and Akanthomyces muscarium from Sunn pests (Eurygaster spp.). Journal of Central European Agriculture, 23(3): 526-532. https://doi.org/10.5513/JCEA01/23.3.3556
Gottel, M.S., Koike, M., Kim, J.J., Aiuchi, D., Shinya, R., Brodeur, J. (2008). Potential of Leacanicillium spp. for management of insects nematodes and plant diseases. Journal of Invertebrate Pathology, 98(3): 256–261. https://doi.org/10.1016/j.jip.2008.01.009
Herron, G., Powis, K., Rophail, J. (2000). Baseline studies and preliminary resistance survey of Australian populations of cotton aphid Aphis gossypii Glover (Hemiptera: Aphididae). Australian Journal of Entomology, 39(1): 33–8. https://doi.org/10.1046/j.1440-6055.2000.00134.x
Herron, G.A., Powis, K., Rophail, J. (2001). Insecticide resistance in Aphis gossypii Glover (Hemiptera: Aphididae), a serious threat to Australian cotton. Australian Journal of Entomology, 40(1): 85–91. https://doi.org/10.1046/j.1440-6055.2001.00200.x
Hollingsworth, R.G., Tabashnik, B.E., Ullman, D.E., Johnson, M.W., Messing, R. (1994). Resistance of Aphis gossypii (Homoptera: Aphididae) to insecticides in Hawaii: spatial patterns and relation to insecticide use. Journal of Economic Entomology, 87(2): 293–300. https://doi.org/10.1093/jee/87.2.293
Karaceylan, Z. (2023). Fungal symbiotic interactions of Ips sexdentatus (Boerner, 1767) with Pinus nigra Arn. in western Mediterranean Region. Süleyman Demirel University, Graduate School of Natural and Applied Sciences, PhD Thesis, 169p.
Kennedy, J.S., Day, M.F., Eastop, V.F. (1962). A conspectus of aphids as vectors of plant viruses. United Kingdom: Commonwealth Inst Entomol., 100–114.
Kim, J.J. (2007). Influence of Lecanicillium attenuatum on the development and reproduction of the cotton aphid, Aphis gossypii. BioControl, 52: 789-799. https://doi.org/10.1007/s10526-006-9050-4
Kumar, K.K., Sridhar, J., Murali-Baskaran, R.K., Senthil-Nathan, S., Kaushal, P., Dara, S.K., Arthurs, S. (2019). Microbial biopesticides for insect pest management in India: current status and future prospects. Journal of Invertebrate Pathology, 165: 74–81. https://doi.org/10.1016/j.jip.2018.10.008
Lacey, L.A., Grzywacz, D., Shapiro-Ilan, D.I., Frutos, R., Brownbridge, M., Goettel, M.S. (2015). Insect pathogens as biological control agents: Back to the future. Journal of Invertebrate Pathology, 132: 1–41. https://doi.org/10.1016/j.jip.2015.07.009
Lord, J.C. (2001). Desiccant dusts synergize the effect of Beauveria bassiana (Hyphomycetes: Moniliales) on stored-grain beetles. Journal of Economic Entomology, 94(2): 367–372. https://doi.org/10.1603/0022-0493-94.2.367
Lu, L., Cheng, B., Du, D., Hu, X., Peng, A., Pu, Z., Zhang, X., Huang, Z., Chen, G. (2015). Morphological, molecular and virulence characterization of three Lecanicillium species infecting Asian citrus psyllids in Huangyuan citrus groves. Journal of Invertebrate Pathology, 125: 45–55. https://doi.org/10.1016/j.jip.2015.01.002
Martin, B., Rahbe, Y., Fereres, A. (2003). Blockage of stylet tips as the mechanism of resistance to virus transmission by Aphis gossypii in melon lines bearing the Vat gene. Annals of Applied Biology, 142: 245-250. https://doi.org/10.1111/j.1744-7348.2003.tb00247.x
Mousavi, M., Ghosta, Y., Maroofpour, N. (2020). Insecticidal activity and sublethal effects of Beauveria bassiana (Bals.-Criv.) Vuill. isolates and essential oils against Aphis gossypii Glover, 1877 (Hemiptera: Aphididae). Acta Agri Slovenica, 115(2): 463–472. https://doi.org/10.14720/aas.2020.115.2.1306
Mnari-Hattab, M., Jebari, H., Zouba, A. (2008). Identification et distribution des virus responsables de mosaiques chez les cucurbitacées en Tunisie. Bulletin OEPP/EPPO Bulletin, 38(3): 497–506. https://doi.org/10.1111/j.1365-2338.2008.01269.x
Nauen, R., Elbert, A. (2003). European monitoring of resistance to insecticides in Myzus persicae and Aphis gossypii (Hemiptera: Aphididae) with special reference to imidacloprid. Bulletin of Entomological Research, 93(1): 47–54. https://doi.org/10.1079/BER2002215
Ramanujam, B., Krishna, J., Poornesha, B., (2018). Field efficacy of entomopathogenic fungi against Brinjal and Chilli aphid (Aphis gossypii Glover) (Homoptera: Aphididae). Pest Research Journal, 30(2): 159-162. https://doi.org/10.5958/2249-524X.2018.00026.2
Razmjou1, J., Davari, M., Ebadollahi, A. (2016). Effect of two plant essential oils and the entomopathogenic fungus, Lecanicillium muscarium (Zare & Gams) on the cotton aphid, Aphis gossypii Glover. Egyptian Journal of Biological Pest Control, 26(4): 775-779.
Revathi, K., Chandrasekaran, R., Tanigaivel, A., Kirubakaran, S.A., Nathan, S.S. (2014). Biocontrol efficacy of protoplast fusants between Bacillus thuringiensis and Bacillus subtilis against Spodoptera litura Fabr. Archives of Phytopathology and Plant Protection, 47(11): 1365–75. https://doi.org/10.1080/03235408.2013.840999
Soetopo, D. (2004). Efficacy of selected Beauveria bassiana (Bals.) Vuill. isolates in combination with a resistant cotton variety (PSB-Ct 9) against the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). https://agris.fao.org/agris-search/search.do?recordID=PH2005000040
Seyedebrahimi, S.S., Talebi Jahromi, K., Imani, S., Hosseini Naveh, V., Hesami, S. (2016). Resistance to imidacloprid in different field populations of Aphis gossypii Glover (hem.: Aphididae) in south of Iran. Journal of Entomological and Acarological Research, 48 (5361): 6–10. https://doi.org/ 10.4081/jear.2016.5361
Tesfaye, D., Seyoum, E. (2010). Studies on the pathogenicity of native entomopathogenic fungal isolates on the cotton / melon aphid, Aphis gossypii (Homoptera: Aphididae) Glover under different temperature regimes. African Entomology, 18(2): 302–312. https://doi.org/10.4001/003.018.0215
Wang, K-Y., Liu, T-X., Yu, C-H., Jiang, X-Y., Yi, M-Q. (2002). Resistance of Aphis gossypii (Homoptera: Aphididae) to fenvalerate and imidacloprid and activities of detoxification enzymes on cotton and cucumber. Journal of Economic Entomology, 95(2): 407–413. https://doi.org/10.1603/0022-0493-95.2.407
Wang, L., Huang, J., You, M., Guan, X., Liu, B. (2007). Toxicity and feeding deterrence of crude toxin extracts of Lecanicillium (Verticillium) lecanii (Hyphomycetes) against sweet potato whitefly, Bemisia tabaci (Homoptera: Aleyrodidae). Pest Management Science, 63: 381–387. https://doi.org/10.1002/ps.1359
Wang, X., Xu, J., Wang, X., Qiu, B., Cuthbertson, A.G.S., Du, C., Wu, J., Ali, S. (2019). Isaria fumosorosea-based-zero-valent iron nanoparticles affect the growth and survival of sweet potato whitefly, Bemisia tabaci (Gennadius). Pest Management Science, 75(8): 2174 –81. https://doi.org/10.1002/ps.5340
Wraight, S.P., Carruthers, R.I., Bradley, C.A., Jaronski, S.T., Lacey, L.A., Wood, P., Wraight, S.G. (1998). Pathogenicity of the entomopathogenic fungi Paecilomyces sp. and Beauveria bassiana against the silver leaf whitefly, Bemisia argentifolii. Journal of Invertebrate Pathology, 71(3): 217–226. https://doi.org/10.1006/jipa.1997.4734
Wraight, S.P., Ramos, M.E. (2002). Application parameter affecting field efficacy of Beauveria bassiana foliar treatments against Colorado potato beetle, Leptnotarsa decemlineata. Biological Control, 23: 164–178. https://doi.org/10.1006/bcon.2001.1004