Achillea millefolium ekstraktının spontan ve oksitosin ile indüklenen izole sıçan uterus kasılmaları üzerine etkileri

Amaç: Achillea millefolium (AM), kadın üreme sistemi üzerindeki etkileri nedeniyle geniş bir coğrafyada geleneksel tıpta yaygın olarak kullanılmaktadır. Ancak uterus düz kas kasılmaları üzerindeki etkisi bilinmemektedir. Çalışmamız AM’nin spontan ve oksitosin kaynaklı izole sıçan uterus kasılmaları üzerindeki etkilerini araştırmayı amaçlamaktadır. Yöntem: Myometrial şeritler 32 yetişkin Wistar Albino sıçanından elde edildi. Kasılma genlikleri ve frekansları, belirtilen uygulamaların ardından izole organ banyosu sisteminde kaydedildi: Yalnızca Krebs-Hanseleit (KBH) çözeltisinin uygulanması, Achillea millefolium ekstraktının (AME) uygulanması; oksitosin ile kasılmaları indükledikten sonra KBH veya AME uygulaması. p<0.05 değeri anlamlı olarak kabul edildi. Bulgular: AM ekstraktı 2mg/ml dozunda uygulandığında, ortama sadece KBH ilavesi ile spontan (uyarılmamış) kasılma sıklığını kontrol grubuna kıyasla önemli ölçüde azalttı (AME: 2.37±0.49, KBH: 9.25±1.69, p=0.002). AME, 0.5, 1 ve 2 mg/ml’lik uygulamalarda spontan uterus kasılmalarının amplitüdünü önemli ölçüde azalttı (p <0.05). Öte yandan AME, artan her dozda oksitosin kaynaklı kasılmaların amplitüdünü önemli ölçüde azalttı (p <0.05). Sonuç: AME’nin doza bağlı olarak hem spontan hem de oksitosin kaynaklı uterus kasılmalarının sıklığını ve amplitüdünü önemli ölçüde azalttığı ortaya çıktı. AME, erken doğum veya düşük riski durumlarında dikkatli kullanılmalıdır.

Anahtar Kelimeler:

Achillea millefolium, oksitosin, oksitosinle indüklenmiş kasılma, uterin kasılma, civanperçemi

Effects of Achillea millefolium extract on spontaneous and oxytocin-induced isolated rat uterine contractions

Objective: Achillea millefolium (AM) is widely used in traditional medicine in a wide geography due to its effects on the female reproductive system. However, its effect on uterine smooth muscle contractions is unknown. Our study aims to investigate the effects of AM on spontaneous and oxytocin-induced isolated rat uterine contractions. Method: Myometrial strips were obtained from 32 adult Wistar Albino rats. Contraction amplitudes and frequencies were recorded by isolated tissue bath system after either of the following: administration of only Krebs-Hanseleit (KBH) solution, administration of Achillea millefolium extract (AME); administration of KBH or AME after inducing contractions with oxytocin. The differences at the level of p<0.05 was considered significant. Results: When AM extract was administered at a dose of 2mg/ml, it significantly reduced the spontaneous (non-induced) contraction frequency compared to the control group with only KBH addition to the medium (AME: 2.37±0.49, KBH: 9.25±1.69, p=0.002). AME significantly reduced the amplitude of spontaneous uterine contractions in the administrations of 0.5, 1, and 2mg/ml (p <0.05). On the other hand, AME significantly reduced the amplitude of oxytocin-induced contractions in each increasing dose (p <0.05). Conclusion: It was revealed that AME significantly reduced the frequency and amplitude of both spontaneous and oxytocin-induced uterine contractions depending on the dose. AME should be used with caution in cases of preterm birth or miscarriage risk.

Keywords:

Achillea millefolium, oxytocin, oxytocin-induced contraction, uterine contraction, yarrow,

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