Fatma GÜR, Ahmet Gökhan AĞGÜL, Mine GÜLABOĞLU

Su ile hazırlanan zeytin yaprağı özütünün ratlarda streptozotosin kaynaklı oksidatif stres ve lipit peroksidasyonu üzerine etkileri

Bu çalışmada, Streptozotosin'in (STZ) neden olduğu diyabetik ratlarda su içinde hazırlanan zeytin yaprağı özütünün (OLE) antidiyabetik ve antioksidatif etkilerinin araştırması amaçlanmıştır. Çalışmamızda Atatürk Üniversitesi Tıbbi Uygulama ve Araştırma Merkezi (ATADEM)’den temin edilen ve ağırlıkları 200-215 g arasında değişen toplam 25 adet Albino Wistar erkek rat kullanıldı. Ratlar Kontrol, 0.5 g kg-1 OLE, 40 mg kg-1 STZ, 0.25 g kg-1 OLE + STZ ve 0.5 g kg-1 OLE + STZ olmak üzere beş gruba ayrıldı. 14 günlük OLE uygulama sonrası ratların karaciğer dokuları süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), glutatyon S-transferaz (GST) aktivitelerinin ve malondialdehit (MDA) seviyelerinin belirlenmesi için alındı. Kan örnekleri ise alanin transaminaz (ALT), aspartat transaminaz (AST) ve alkalen fosfataz (ALP) biyokimyasal parametrelerinin ve ek olarak glikoz seviyelerinin belirlenmesi için alındı. İstatistiksel analizler SPSS 20.0 yazılım programı kullanılarak ANOVA testi ile belirlendi. STZ ile birlikte OLE’nin iki farklı dozda oral yoldan verilmesinden sonra, diyabetik grup ile karşılaştırıldığında OLE’nin her iki doz grubunda ALP, ALT ve AST düzeylerinde önemli düşüşler gözlenmiştir (p<0.001). Ayrıca, sadece STZ verilen gruba kıyasla, STZ ile birlikte OLE uygulanan gruplarda SOD, GPx ve GST aktivitelerinde anlamlı bir artışla birlikte MDA, ALT, AST ve ALP düzeylerinde istatiksel olarak anlamlı düşüşler görülmüştür (p<0.001). Ancak OLE’nin 0.5 g kg-1 dozu 0.25 g kg-1 dozuna kıyasla daha etkili olmuştur. Sonuç olarak, elde edilen in-vivo veriler, suda hazırlanan OLE'nin oksidatif stresin kısıtlanması ve antioksidan aktivitelerin güçlendirilmesi sayesinde bir anti-diyabetik etkiye sahip olabileceğini göstermiştir.

Anahtar Kelimeler:

Streptozotosin, Diabetes Mellitus, Zeytin yaprağı özütü, antioksidan, rat

Effects of olive leaf extract prepared in water on streptozotocin-induced oxidative stress and lipid peroxidation in rats

The present study aims to investigate antidiabetic and antioxidative effects of olive leaf extract (OLE) prepared in water in Streptozotocin (STZ)-induced diabetes in experimental rats. 25 Albino Wistar male rats, each weighing 200-215 g, were used for the experiment after being obtained from Medical Experimental Application and Research Centre (ADEM). The animals were divided into five groups: Control Group, 0.5 g kg-1 OLE Alone Group, 40 mg kg-1 STZ Alone Group, 0.25 g kg-1 OLE+STZ Group (T1), and 0.5 g kg-1 OLE+STZ Group (T2). Following the application of OLE for fourteen days, the livers of the animals were dissected to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), Glutathione S-transferases (GST) in addition to malondialdehyde (MDA) levels. Besides, their blood samples were obtained to investigate the biochemical parameters of alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) apart from glucose levels. Statistical analyses were performed via the ANOVA test using the SPSS 20.0 software program. After an oral administration of OLE in two different doses along with STZ, there was a significant decrease in ALT, AST and ALP levels of the animals in the high-dose group (T2: OLE+STZ) compared to the diabetic group (STZ) (p <0.001). After the administration of OLE with STZ in two different doses, there were significant changes in the ALT, AST and ALP levels of T1 and T2 Groups when compared to the diabetic group (p<0.001). Besides, compared to STZ Alone Group, the SOD, GPx and GST activities significantly increased while MDA, ALT, AST and ALP levels significantly decreased (p <0.001). However, 0.5 g kg-1 of OLE was more effective than was 0.25 g kg-1. As a result, the in-vivo data showed that OLE dissolved in water could have an anti-diabetic effect by restricting oxidative stress and reinforcing antioxidant activities.

Keywords:

Streptozotosin, Diabetes Mellitus, rat, Olive Leaf Extract, Antioxidant,

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Abdel-Kader MS, Soliman GA, Abdel-Rahman RF, Saeedan AS, Abd-Elsalam RM, & Ogaly HA, 2019. Effect of olive leaves extract on the antidiabetic effect of glyburide for possible herb-drug interaction. Saudi Pharmaceutical Journal, 27(8), 1182-1195.
ADD, 2014. American Diabetes Association.
Afify A, El-Beltagi H, Fayed, S, & El-Ansary A, 2018. In vivo correlation of olive leaves extract on some oxidative stress markers in streptozotocin-induced diabetes mellitus in rats. Grasas y Aceites, 69(1), 243.
Al-Attar AM, & Alsalmi FA, 2019. Effect of Olea europaea leaves extract on streptozotocin induced diabetes in male albino rats. Saudi journal of biological sciences, 26(1), 118-128.
Baz H (2014). Streptozotocinle indüklenen diyabetli ratlar üzerinde myrtus communis l. Yaprağı su ekstresi etkilerinin araştırılması.
Benavente-Garcia O, Castillo J, Lorente J, & Alcaraz M, 2002. Radioprotective effects in vivo of phenolics extracted from Olea europaea L. leaves against X-ray-induced chromosomal damage: comparative study versus several flavonoids and sulfur-containing compounds. J Med Food, 5(3), 125-135.
Benavente-Garcı́a O, Castillo J, Lorente J, Ortuño A, & Del Rio JA, 2000. Antioxidant activity of phenolics extracted from Olea europaea L. leaves. Food Chemistry, 68(4), 457-462.
Bouaziz M, Chamkha M, & Sayadi S, 2004. Comparative study on phenolic content and antioxidant activity during maturation of the olive cultivar Chemlali from Tunisia. Journal of Agricultural and Food Chemistry, 52(17), 5476-5481.
Bouaziz M, & Sayadi S, 2005. Isolation and evaluation of antioxidants from leaves of a Tunusian cultivar olive tree. Eur. J. Lipid Sci. Technol., 107, 497-504.
Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2), 248-254.
Burtis CA, & Ashwood ER, 1999. Tietz Textbook of Clinical Chemistry. Philadelphia, Pennsylvania W.B. Saunders Company.
Cengiz M, Ayhanci A, Kutlu HM, & Musmul A. (2016). Potential therapeutic effects of silymarin and silymarin-loaded solid lipidnanoparticles on experimental kidney damage in BALB/c mice: biochemical and histopathological evaluation. Turkish Journal of Biology, 40(4), 807-814.
Coşkunırmak D, 2018. Diyabetik ayak yarası gelişen hastaların geleneksel ve tamamlayıcı tıp uygulamalarını kullanma durumlarının incelenmesi. Adnan Menderes Üniversitesi Sağlık Bilimleri Enstitüsü.
Dawn BM, Allan DM, & Colleen MS, 1996. Basic Medical Biochemistry a Clinical Approach. Baltimore, Maryland Lippincott Williams & Wilkins.
Eidi A, Eidi M, & Darzi, R 2009. Antidiabetic effect of Olea europaea L. in normal and diabetic rats. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 23(3), 347-350.
El-Amin M, Virk P, Elobeid M, Almarhoon ZM, Hassan ZK, Omer SA, . . . Al-Olayan EM, 2013. Anti-diabetic effect of Murraya koenigii (L) and Olea europaea (L) leaf extracts on streptozotocin induced diabetic rats. Pak J Pharm Sci, 26(2), 359-365.
Fantel AG, 1996. Reactive oxygen species in developmental toxicity: review and hypothesis. Teratology, 53(3), 196-217.
Habig WH, Pabst MJ, and Jakoby WB, 1974. "Glutathione S-transferases the first enzymatic step in mercapturic acid formation." Journal of biological Chemistry 249(22), 7130-7139.
Jain SK, McVie R, Duett J, and Herbst JJ, 1989. "Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes." Diabetes 38(12), 1539-1543.
Jemai H, El Feki A, & Sayadi S, 2009. Antidiabetic and antioxidant effects of hydroxytyrosol and oleuropein from olive leaves in alloxan-diabetic rats. Journal of agricultural and food chemistry, 57(19), 8798-8804.
Kritsakis A, 1998. Olive oil – From the tree to the table. Trumbull, CT (USA): Food and Nutrition Press, Inc.
Macdonald G, 2008. Harrison’s Internal Medicine, 17th edition. - by A. S. Fauci, D. L. Kasper, D. L. Longo, E. Braunwald, S. L. Hauser, J. L. Jameson and J. Loscalzo. Internal Medicine Journal, 38(12), 932-932.
Paglia DE and Valentine WN, 1967. "Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase." The Journal of laboratory and clinical medicine 70(1), 158-169.
Pereira JA, Pereira AP, Ferreira IC, Valentao P, Andrade PB, Seabra R. . . . Bento A, (2006). Table olives from Portugal: phenolic compounds, antioxidant potential, and antimicrobial activity. Journal of Agricultural and Food Chemistry, 54(22), 8425-8431.
Rabbani SI, Devi K, & Khanam S, 2010. Protective role of glibenclamide against nicotinamide-streptozotocin induced nuclear damage in diabetic Wistar rats. Journal of pharmacology & pharmacotherapeutics, 1(1), 18.
Saddala RR, Thopireddy L, Ganapathi N, & Kesireddy SR, 2013. Regulation of cardiac oxidative stress and lipid peroxidation in streptozotocin-induced diabetic rats treated with aqueous extract of Pimpinella tirupatiensis tuberous root. Experimental and Toxicologic Pathology, 65(1), 15-19.
Soliman GA, Saeedan AS, Abdel-Rahman RF, Ogaly HA, Abd-Elsalam, RM, & Abdel-Kader MS, 2019. Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study. Saudi Pharmaceutical Journal, 27(3), 326-340.
Stadtman ER, 2004. Role of oxidant species in aging. Current Medicinal Chemistry, 11(9), 1105-1112.
Sun Y, Oberley LW, and Li Y, 1988. "A simple method for clinical assay of superoxide dismutase." Clinical chemistry 34(3), 497-500.
Tang SY, Sivakumar M, Ng, AM-H, & Shridharan P, 2012. Anti-inflammatory and analgesic activity of novel oral aspirin-loaded nanoemulsion and nano multiple emulsion formulations generated using ultrasound cavitation. International journal of pharmaceutics, 430(1-2), 299-306.
Taysi S, Memisogullari R, Koc M, Yazici AT, Aslankurt M, Gumustekin K, . . .Ozder TH, 2008. Melatonin reduces oxidative stress in the rat lens due to radiation-induced oxidative injury. International Journal of Radiation Biology, 84(10), 803-808.
Temple NJ, 2000. Re: "Dietary flavonoid intake and risk of cardiovascular disease in postmenopausal women". American Journal of Epidemiology, 151(6), 634-635.
Tietz NW, 1995. Clinical Guide to Laboratory Tests. Philadelphia, Pennsylvania: W.B. Saunders Company.
Visioli F, Poli A, & Gall C, 2002. Antioxidant and other biological activities of phenols from olives and olive oil. Medicinal research reviews, 22(1), 65-75.
Yanbeyi S, 1999. Aspirin ve antioksidant buthylated hydroxyanisole’ ün tavşanlarda eritrosit total katalaz, süperoksit dismutaz ve glutatyon peroksidaz aktiviteleri üzerine etkileri. Ondokuz Mayıs Üni. , Samsun.
Yoruk O, Gur F, Uyanik H, Yasar M, Mutlu V, Altas E, . . . Taysi S, 2010. Antioxidant effects of Nigella sativa in the treatment of experimentally induced rhinosinusitis. Macedonian Journal of Medical Sciences, 3(2), 132-137.