Rumex crispus Ekstraktı Sıçanlarda Streptozotosin ile Oluşturulan Diyabette Anti-Diyabetik Özellikler Gösterir

Rumex crispus, nehir kenarı ve ıslak çayırlarda kendiliğinden yetişen ve alternatif tıpta yüzyıllardır kullanılan doğal, yabani bir bitkidir. Bu çalışmada, streptozotosin (STZ) aracılığıyla diyabet oluşturulan sıçanlarda R. crispus'un canlı ağırlıkları, açlık kan glukoz seviyeleri, plazma lipid profili, karaciğer enzim aktiviteleri ve antioksidan sisteme etkileri araştırıldı. Hayvanlar 4 gruba ayrıldı. I. grup: kontrol; II. grup: 3 mg kg-1 R. crispus; grup III: 50 mg kg-1 Streptozotosin (STZ) ile diyabet oluşturulan grup; grup IV: 50 mg kg-1 STZ ile diyabet oluşturulan + 3 mg kg-1 R. crispus uygulanan grup şeklinde belirlendi. R. crispus ekstraktının, diyabet oluşumundan 10 gün sonra diyabete bağlı ağırlık kaybını ve kan glikoz seviyesini azalttığı tespit edildi. 14 günlük uygulama süresinin sonunda, R. crispus uygulanan diyabetik sıçanlarda trigliserit, LDL kolesterol (LDL-C) ve VLDL kolesterol (VLDL-C) düzeyleri artarken (P<0.001), HDL kolesterol (HDL-C) seviyeleri anlamlı derecede azaldı (P<0.01). Alanin aminotransferaz (ALT) ve aspartat aminotransferaz (AST) seviyeleri, R. crispus'a cevaben önemli ölçüde artmıştır (P<0.001). Diyabetik grupta total antioksidan seviye (TAS) anlamlı derecede azalırken, total oksidan seviyenin (TOS) artış gösterdiği belirlendi. Bununla birlikte, R. crispus tedavisine cevap olarak, TAS anlamlı şekilde artmış ve TOS kontrol grubuna göre azalmıştır (her ikisi için de P<0.001). R. crispus ekstraktının takviyesi, sıçanlarda anti-diyabetik özellikler gösterir ve diyabetik bireyler için klinik bir potansiyele sahip olabilir.

Anahtar Kelimeler:

Diyabet, lipit profili, karaciğer enzimleri, oksidatif durum, Rumex crispus

Rumex crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats

Rumex crispus is a natural, wild plant that grows spontaneously on riverside and wet meadows, and has been used for centuries in alternative medicine. In the present study, the effects of R. crispus on body weights, fasting blood glucose levels, plasma lipid profile, liver enzyme activities and antioxidant system were investigated in streptozotocin (STZ) induced diabetic rats. The animals were divided into 4 groups. Group I: control; group II: 3 mg kg-1 R. crispus; group III: 50 mg kg-1 Streptozotocin (STZ)-induced diabetes; group IV: 50 mg kg-1 STZ-induced diabetes + 3 mg kg-1 R.crispus. It was found that R. crispus extract reduced diabetes-related weight loss and blood glucose levels 10 days after the formation of diabetes. At the end of 14-day treatment period, HDL cholesterol (HDL-C) levels significantly decreased (P<0.01) while triglyceride, LDL cholesterol (LDL-C) and VLDL cholesterol (VLDL-C) levels increased in R. crispus supplemented diabetic rats (P<0.001). The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly increased in response to R. crispus (P <0.001). Total antioxidant status (TAS) significantly decreased and total oxidant status (TOS) increased in diabetic group; however, in response to R. crispus treatment, TAS significantly increased and TOS decreased relative to control group (P<0.001, for both). Supplementation of R. crispus extract shows anti-diabetic properties in rats and might have a clinical potential for diabetic individuals.

Keywords:

Diabetes, lipid profile, liver enzymes, oxidative status, Rumex crispus,

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