Oğuz ÇAKIR, Monireh BAKHSHPOUR, Fatma YILMAZ, Zübeyde BAYSAL

Pestisit Tayini için Nanopartiküllerle Duyarlılığı Arttırılmış Moleküler Baskılama Temelli Yüzey Plazmon Rezonans Sensörlerin Hazırlanması

Pestisitler yıllardır tarım alanlarında kullanılmaktadır. Ancak bu yaygın kullanım, bilimsel ve endüstriyel

Anahtar Kelimeler:

Moleküler baskılama, nanopartikül, pestisit, yüzey plazmon rezonans sensör

Preparation of Nanoparticle-Amplified Surface Plasmon Resonance Sensors Based on Molecular Imprinting for Pesticide Determination

Pesticides have been utilized in agriculture for decades. However, their widespread use has increasedconcerns due to their known toxicities on long term human health risks in scientific and industrial communities.Thus, the detection of pesticides will have a great prescription due to both improve their toxicity effects overhumans and their management. In this study, poly[ethyleneglycol dimethacrylate-N-methacryloyl-(L)-tryptophanmethyl ester poly(EGDMA-MATrp) nanoparticles for sensitive, selective, fast and realtime detection chlorpyrifoswere firstly prepared and characterized by zeta sizer. Then, a chlorpyrifos-printed SPR nanosensor was prepared andcharacterized by atomic force microscopy (AFM) and contact angle measurements. The thickness measurementsand AFM observations indicated that the nanoparticle thin films were almost monolayer. Chlorpyrifos sensingability of imprinted and nonimprinted nanosensors were investigated from chlorpyrifos solutions within the rangeof 0.015-2.9 nM. Imprinted nanoparticles showed more sensitivity to chlorpyrifos than non-imprinted ones.Langmuir adsorption model was determined as the most suitable model for this affinity system. In order to showthe selectivity of the chlorpyrifos imprinted nanoparticles, competitive adsorption of chlorpyrifos, diazinon andparathion at 2.9 nM concentration was investigated. As a result of, SPR nanosensors have a potential to be used asan alternative method for pesticides analysis due to fast response, easy-to-use properties, precision, selective andreal-time measurement capability.

Keywords:

Molecular imprinting, nanoparticles, pesticide, surface plasmon resonance sensor,

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