Krom’un Deinococcus radiodurans ve Rekombinantlarına Etkisi

Ağır metaller düşük konsantrasyonlarda bulunmaları durumunda bile canlı organizmalar için toksik etkiye sahip olabilirler. Canlı metabolizmasında önemli bir yeri olan krom, genellikle Cr (III) formunda bulunmaktadır. Cr (VI) ise endüstriyel faaliyetler sonucunda ortaya çıkmaktadır. Son yıllarda ciddi çevre problemlerine neden olan ağır metallerin ve ksenobiyotiklerin detoksifikasyonuyla ilgili yoğun araştırmalar yapılmaktadır. Özellikle bu maddeleri daha zararsız bileşiklere çevirmek için gerekli olan genetik bilgiye sahip bakteri türlerinin alternatif kullanım alanı bulabileceği anlaşılmıştır. Bu amaçla çalışmamızda, yabanıl Deinococcus radiodrans ile Vitreoscilla hemoglobin (vgb) geni klonlanmış rekombinantı ve kontrol olarak da vgb¯ rekombinant suşu kullanılmıştır. Bu sayede D. radiodurans ve rekombinantlarının üreme potansiyelleri üzerine Cr‟un etkisi araştırılarak bakterilerin metal uygulaması öncesi ve sonrası morfolojik değişimleri SEM fotoğrafları ile saptanmıştır. Bunlara ek olarak bakteriye daha fazla oksijenli ortam sağlayan vgb geninin, bakterinin üreme yeteneğine yapacağı katkı araştırılmıştır. Çalışmamızın sonunda, bakterilerin üreme potansiyelleri üzerinde vgb geninin bakteriye daha fazla oksijenli ortam yaratıp daha fazla üremesini sağlayarak meydana getirdiği olumlu etkiler gözlenmiştir.

Anahtar Kelimeler:

Deinococcus radiodurans, krom (Cr), Vitreoscilla hemoglobin

The Effect of Chrome on Deinococcus radiodurans and Recombinants

Heavy metals can have toxic effects on living organisms even in low concentrations. Having an important place in the living metabolism, the chromium is generally in Cr (III) form. As per the Cr (VI), it emerges as a result of industrial activities. In recent years, intensive research studies have been conducted about heavy metals causing serious environmental problems and detoxification of xenobiotics. It is appeared that the bacteria species, which are equipped with genetic information necessary for breaking down particularly these substances into less harmful compounds, can be an alternative area of use. In our study, we used cloned recombinant Vitreoscilla hemoglobin (vgb) gene with wild Deinococcus radiodurans and vgb- recombinant strain as control. By this means, the effect of Cr on the reproductive potential of D. radiodurans and its recombinants were examined. The morphological changes of the bacteria before and after the metal application were detected through SEM photos. In addition to these, the contribution of vgb gene, which provides a more aerobic (oxygenated) environment for the bacteria, was examined. As the conclusion of our study, positive effects of vgb genes on the reproductive potentials of the bacteria were observed, which ensured a higher reproduction rate through providing a more oxygenated environment for the bacteria.

Keywords:

Deinococcus radiodurans, chromium (Cr), Vitreoscilla hemoglobin,

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