Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells
Cancer is a big public health problem in many parts of the world. A novel anti-tumor protein (J2-C2) was previously isolated from Arca inflata and it was reported that this protein had anti-proliferative effect on some human tumor cell lines such as A549, HepG2 and SPC-A-1. In this study, firstly, J2-C2 was produced by recombinant techniques in the Escherichia coli strain BL21 (DE3) pLysE and this protein was purified by Ni-NTA agarose affinity chromatography. Expressed recombinant J2-C2 was analyzed with SDS-PAGE. 75.5 mg ml-1 of J2-C2 was achieved from a 600 mL culture. Then using HT-29, MCF7 and PC3 cancer cell lines, we showed the effect of recombinant of J2-C2 on cell proliferation, migration and apoptosis in a cell specific manner. Cell viability was measured using MTT assay. Additionally, real-time-qPCR was applied to analyze the transcript levels of apoptosis related genes such as Bcl-2, Bax and p53. The 2–ΔΔCt method was performed to determine the relative changes in gene transcription. Moreover, scratch wound healing assay was performed to evaluate the effect of J2-C2 on cancer cell migration. Consequently, we found that recombinant J2-C2 did not have a significant effect on cell viabilities of MCF7, PC3 and HT29 in concentration-dependent manner. Furthermore, our results showed that recombinant J2-C2 declined HT29, MCF7 cell migration. However, we did not observe the same results for PC3 cancer cell line.
Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells
Cancer is a big public health problem in many parts of the world. A novel anti-tumor protein (J2-C2) was previously isolated from Arca inflata and it was reported that this protein had anti-proliferative effect on some human tumor cell lines such as A549, HepG2 and SPC-A-1. In this study, firstly, J2-C2 was produced by recombinant techniques in the Escherichia coli strain BL21 (DE3) pLysE and this protein was purified by Ni-NTA agarose affinity chromatography. Expressed recombinant J2-C2 was analyzed with SDS-PAGE. 75.5 mg ml-1 of J2-C2 was achieved from a 600 mL culture. Then using HT-29, MCF7 and PC3 cancer cell lines, we showed the effect of recombinant of J2-C2 on cell proliferation, migration and apoptosis in a cell specific manner. Cell viability was measured using MTT assay. Additionally, real-time-qPCR was applied to analyze the transcript levels of apoptosis related genes such as Bcl-2, Bax and p53. The 2–ΔΔCt method was performed to determine the relative changes in gene transcription. Moreover, scratch wound healing assay was performed to evaluate the effect of J2-C2 on cancer cell migration. Consequently, we found that recombinant J2-C2 did not have a significant effect on cell viabilities of MCF7, PC3 and HT29 in concentration-dependent manner. Furthermore, our results showed that recombinant J2-C2 declined HT29, MCF7 cell migration. However, we did not observe the same results for PC3 cancer cell line.
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| Bibtex | @araştırma makalesi { jist730267, journal = {Journal of the Institute of Science and Technology}, issn = {2146-0574}, eissn = {2536-4618}, address = {}, publisher = {Iğdır Üniversitesi}, year = {2020}, volume = {10}, pages = {2943 - 2951}, doi = {10.21597/jist.730267}, title = {Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells}, key = {cite}, author = {Songur, Mustafa and Bilgin, Sema and Erden Tayhan, Seçil and Gökçe, İsa} } |
| APA | Songur, M , Bilgin, S , Erden Tayhan, S , Gökçe, İ . (2020). Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells . Journal of the Institute of Science and Technology , 10 (4) , 2943-2951 . DOI: 10.21597/jist.730267 |
| MLA | Songur, M , Bilgin, S , Erden Tayhan, S , Gökçe, İ . "Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells" . Journal of the Institute of Science and Technology 10 (2020 ): 2943-2951 < |
| Chicago | Songur, M , Bilgin, S , Erden Tayhan, S , Gökçe, İ . "Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells". Journal of the Institute of Science and Technology 10 (2020 ): 2943-2951 |
| RIS | TY - JOUR T1 - Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells AU - Mustafa Songur , Sema Bilgin , Seçil Erden Tayhan , İsa Gökçe Y1 - 2020 PY - 2020 N1 - doi: 10.21597/jist.730267 DO - 10.21597/jist.730267 T2 - Journal of the Institute of Science and Technology JF - Journal JO - JOR SP - 2943 EP - 2951 VL - 10 IS - 4 SN - 2146-0574-2536-4618 M3 - doi: 10.21597/jist.730267 UR - Y2 - 2020 ER - |
| EndNote | %0 Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells %A Mustafa Songur , Sema Bilgin , Seçil Erden Tayhan , İsa Gökçe %T Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells %D 2020 %J Journal of the Institute of Science and Technology %P 2146-0574-2536-4618 %V 10 %N 4 %R doi: 10.21597/jist.730267 %U 10.21597/jist.730267 |
| ISNAD | Songur, Mustafa , Bilgin, Sema , Erden Tayhan, Seçil , Gökçe, İsa . "Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells". Journal of the Institute of Science and Technology 10 / 4 (Aralık 2020): 2943-2951 . |
| AMA | Songur M , Bilgin S , Erden Tayhan S , Gökçe İ . Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells. Iğdır Üniv. Fen Bil Enst. Der.. 2020; 10(4): 2943-2951. |
| Vancouver | Songur M , Bilgin S , Erden Tayhan S , Gökçe İ . Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells. Journal of the Institute of Science and Technology. 2020; 10(4): 2943-2951. |
| IEEE | M. Songur , S. Bilgin , S. Erden Tayhan ve İ. Gökçe , "Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells", Journal of the Institute of Science and Technology, c. 10, sayı. 4, ss. 2943-2951, Ara. 2020, doi:10.21597/jist.730267 |
- ISSN: 2146-0574
- Yayın Aralığı: Yılda 4 Sayı
- Yayıncı: Iğdır Üniversitesi
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