Dimetil Benzantrasen Uygulanan Ratlarda Tunceli Dağ Sarımsağı (Allium tuncelianum) ve Vitamin E‘nin Bağırsak Dokusundaki Lipit Peroksidasyonu ve Antioksidan Enzim Aktivitelerine Etkisi

Bu çalışmada; 7,12-dimethyl benzanthracene (DMBA) ile indüklenen ratlara Tunceli Dağ Sarımsağı (Allium tuncelianum) ve vitamin E uygulamasının, rat bağırsak dokusundaki lipit peroksidasyon ve antioksidan enzim aktivitelerine etkisinin belirlenmesi amaçlandı. Çalışmada, Wistar albino tipi dişi ratlar kullanıldı. Her grupta 8 adet (n=8) hayvan olmak üzere, 5 grup oluşturuldu. 1.Grup; Kontrol grubu, 2.Grup; 7,12-dimethyl benzanthracene (DMBA) verilen grup, 3.Grup; 7.12-DMBA’ya ilaveten 250 mg/kg/gün Tunceli Dağ Sarımsağı (TDS) verilen grup, 4.Grup; 7.12-DMBA’ya ilaveten 500 mg/kg/gün TDS verilen grup ve 5.Grup; 7.12-DMBA’ya ilaveten 200 mg/kg (haftada iki kez) E vitamini verilen gruptu. 30 günlük deneme sonunda; ratlara ötenazi uygulandı ve bağırsak dokusu çıkarılarak homojenize edildi. Bağırsak doku homojenatındaki lipit peroksidasyon ve antioksidan enzim düzeyi spektrofotometrik yöntemle ölçüldü. Çalışma sonucunda DMBA uygulanan grubun (Grup 2) bağırsak dokusunda: katalaz (CAT), total glutatyon (GSH) ve süperoksit dismutaz (SOD) enzim aktivitesinin kontrol grubuna göre anlamlı olarak azaldığı (P

Anahtar Kelimeler:

Allium tuncelianum, Antioksidan enzim aktivitesi, DMBA, MDA

The Effect of Tunceli Mountain Garlic (Allium tuncelianum) and Vitamin E on Lipid Peroxidation and Antioxidant Enzyme Activities in the Intestinal Tissue of Dimethyl Benzanthracene-Administered Rats

The aim of this study was to determine the effect of Tunceli Mountain Garlic (Allium tuncelianum) and vitamin E on lipid peroxidation and antioxidant enzyme activities in rat intestinal tissue. Wistar albino type female rats were used in the study. Five groups, included 8 animals (n=8) in each, were formed. Group 1; Control group, Group 2; 7,12-dimethyl benzanthracene (DMBA) given group, Group 3; 7,12-DMBA in addition to 250 mg/kg/day Tunceli Mountain Garlic (TDS) given group, Group 4; 7,12-DMBA plus 500 mg/kg/day TDS given group and Group 5; 7,12-DMBA in addition to vitamin E 200 mg/kg (twice a week) was formed as a group. At the end of the 30-days trial rats were euthanized and intestinal tissue was removed and homogenized.Lipid peroxidation and antioxidant enzyme levels were measured in intestinal tissue homogenate by spectrophotometric method. At the end of the study, it was determined that catalase (CAT), total glutathione (GSH) and superoxide dismutase (SOD) enzyme activities in intestinal tissue of DMBA given group (Group 2) significantly decreased compared to the control group (P

Keywords:

Allium tuncelianum, Antioxidant enzyme activity, DMBA, MDA,

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